Fraction Collection Solutions
World Wide Web: www.gilson.com
E-mail: [email protected], [email protected], [email protected]
World Headquarters
Gilson, Inc.
3000 W. Beltline Hwy., P.O. Box 620027, Middleton, WI 53562-0027, USA
Telephone: (1) 800-445-7661 or (1) 608-836-1551 • Fax: (1) 608-831-4451
Gilson S.A.
72 rue Gambetta, B.P. 45
95400 Villiers-le-Bel, France
Telephone: (33) 1-34-29-50-00 • Fax: (33) 1-34-29-50-80
303034-02, Printed in the U.S.A., February 2000, Specifications subject to change without notice
ISO 9001
Certified
Automated Fraction Collection
for HPLC or LC/MS Purification
Fraction
Collection
Solutions
Automated Fraction Collection
for HPLC or LC/MS Purification
215 NEBULA Series
Fraction Collection
A complete line of collection instruments
Injection and collection on the same bed
Gilson, Inc. provides the capability to fully integrate any HPLC analysis system with a
complete line of collection instruments. The demand for high throughput and high purity are essential in today's rapidly changing research and development environment.
Sophisticated, yet reliable, fraction collection from Gilson provides the integral solutions
to keep up with the heavy workload and to eliminate purification bottlenecks.
Gilson's complete line of fraction collectors allows for a wide range of collection options
from large bed collection on the FC 204 to injection, collection and re-injection on the
same bed with the 215 Fraction Collector. Choose between a broad array of collection routines from simple time, drop and peak collection to sophisticated multi-mode operation.
Customize your purification system with numerous collection vessels ranging from
microplates and microcentrifuge tubes to standard test tubes and more. A variety of control options are available for either stand-alone use or software control for easy integration into an HPLC or LC/MS system.
™
The 215 injector’s architecture allows for injection and
collection on the same bed when used in Gilson
combinatorial chromatography systems
Gilson’s UniPoint™ System Software features graphical
sample tracking, matching each sample position with
its corresponding fraction(s) and chromatogram
Flexible collection methods: collect fractions by time,
volume, peak collection by level, peak collection by
slope or subfractions by time
Re-inject collected fractions to verify peak purity
The 215’s movable z-arm provides for more accurate
sample collection into any collection vessel, especially
microplates, without residual splashing and
contamination in neighboring wells
215 Fraction Collector
Ideal as a large bed fraction collector, the 215 Fraction
Collector handles up to seventeen 96-well microplates,
480 vials or 480 tubes
Reliable, robust and accurate, the 215 has proven itself
in the laboratory and is quickly becoming the industry
standard for high throughput applications
215 Injector
Page 2
Fraction
Collection
Solutions
Automated Fraction Collection
for HPLC or LC/MS Purification
215 NEBULA Series
Fraction Collection
A complete line of collection instruments
Injection and collection on the same bed
Gilson, Inc. provides the capability to fully integrate any HPLC analysis system with a
complete line of collection instruments. The demand for high throughput and high purity are essential in today's rapidly changing research and development environment.
Sophisticated, yet reliable, fraction collection from Gilson provides the integral solutions
to keep up with the heavy workload and to eliminate purification bottlenecks.
Gilson's complete line of fraction collectors allows for a wide range of collection options
from large bed collection on the FC 204 to injection, collection and re-injection on the
same bed with the 215 Fraction Collector. Choose between a broad array of collection routines from simple time, drop and peak collection to sophisticated multi-mode operation.
Customize your purification system with numerous collection vessels ranging from
microplates and microcentrifuge tubes to standard test tubes and more. A variety of control options are available for either stand-alone use or software control for easy integration into an HPLC or LC/MS system.
™
The 215 injector’s architecture allows for injection and
collection on the same bed when used in Gilson
combinatorial chromatography systems
Gilson’s UniPoint™ System Software features graphical
sample tracking, matching each sample position with
its corresponding fraction(s) and chromatogram
Flexible collection methods: collect fractions by time,
volume, peak collection by level, peak collection by
slope or subfractions by time
Re-inject collected fractions to verify peak purity
The 215’s movable z-arm provides for more accurate
sample collection into any collection vessel, especially
microplates, without residual splashing and
contamination in neighboring wells
215 Fraction Collector
Ideal as a large bed fraction collector, the 215 Fraction
Collector handles up to seventeen 96-well microplates,
480 vials or 480 tubes
Reliable, robust and accurate, the 215 has proven itself
in the laboratory and is quickly becoming the industry
standard for high throughput applications
215 Injector
Page 2
FC 203 and FC 204
Choose the Capacity and Collection
Volume to Meet Your Purification Needs
Operate either the FC 203 or the FC 204 as stand-alone
instruments or control them by computer as an
integrated part of an automated system
Add up to ten collection windows or selective peak
collection windows to collect only the column
eluent of interest
The FC 203 and FC 204’s built-in keypads make setup
and operation simple and efficient
Two contact closure inputs allow remote start-up,
advance and stoppage of the collectors
Collect fractions by time, drop or peak with ease;
manual collection is also available
The FC 204 is ideal for a dedicated, large-bed fraction
collector. It holds up to four hundred thirty-two 10
mm test tubes or eight 96-well microplates
Simply set the time or number of drops, press START
and walk away
In peak mode, the collectors monitor a detector signal
and identify peaks using either an adaptive-slope
algorithm or a specified millivolt cutoff
When collecting peaks by slope, the software
compensates for drifting baselines, negative peaks and
merged peaks
The FC 203 is the perfect compact fraction collector,
capable of handling a 96-well microplate, 0.5 ml and
1.5 ml microcentrifuge tubes or scintillation vials, in
addition to standard 12 mm or 13 mm test tubes
FC 204
Gilson’s fraction collectors are cold room compatible
for added flexibility
In peak mode, customize collection to the specific peak
architecture of a run by subfractionating peaks by time
or drop. By using different time/drop volumes for peak
and non-peak eluent, collect between peak eluent in
standard test tubes, scintillation vials and more,
providing your laboratory with the added flexibility
that today’s high throughput requirements demand
Three-way diverter valve is standard on the FC 204 and optional
on the FC 203. This valve redirects non-peak eluent directly to
waste assuring precise peak cutting.
FC 203
Options for the FC 203 and FC 204
Multiple column adapter increases throughput and saves time by
allowing simultaneous collection from as many as eight separate
columns on the FC 203 and eighteen columns on the FC 204
Page 4
FC 203 and FC 204
Choose the Capacity and Collection
Volume to Meet Your Purification Needs
Operate either the FC 203 or the FC 204 as stand-alone
instruments or control them by computer as an
integrated part of an automated system
Add up to ten collection windows or selective peak
collection windows to collect only the column
eluent of interest
The FC 203 and FC 204’s built-in keypads make setup
and operation simple and efficient
Two contact closure inputs allow remote start-up,
advance and stoppage of the collectors
Collect fractions by time, drop or peak with ease;
manual collection is also available
The FC 204 is ideal for a dedicated, large-bed fraction
collector. It holds up to four hundred thirty-two 10
mm test tubes or eight 96-well microplates
Simply set the time or number of drops, press START
and walk away
In peak mode, the collectors monitor a detector signal
and identify peaks using either an adaptive-slope
algorithm or a specified millivolt cutoff
When collecting peaks by slope, the software
compensates for drifting baselines, negative peaks and
merged peaks
The FC 203 is the perfect compact fraction collector,
capable of handling a 96-well microplate, 0.5 ml and
1.5 ml microcentrifuge tubes or scintillation vials, in
addition to standard 12 mm or 13 mm test tubes
FC 204
Gilson’s fraction collectors are cold room compatible
for added flexibility
In peak mode, customize collection to the specific peak
architecture of a run by subfractionating peaks by time
or drop. By using different time/drop volumes for peak
and non-peak eluent, collect between peak eluent in
standard test tubes, scintillation vials and more,
providing your laboratory with the added flexibility
that today’s high throughput requirements demand
Three-way diverter valve is standard on the FC 204 and optional
on the FC 203. This valve redirects non-peak eluent directly to
waste assuring precise peak cutting.
FC 203
Options for the FC 203 and FC 204
Multiple column adapter increases throughput and saves time by
allowing simultaneous collection from as many as eight separate
columns on the FC 203 and eighteen columns on the FC 204
Page 4
Fraction Collector Specifications
202C
High capacity, versatile,
multi-mode fraction collection
Large bed, high capacity, cold-room compatible fraction
collection with a wide variety of standard and nonstandard racks
215 NEBULA Series™
Fraction Collection
FC 204
FC 203
202C
Injection and collection
high capacity
medium capacity
versatile
Numerous flexible programming
options available through UniPoint™
System Software
Up to 10 collection windows and 10
drain steps in any mode.
Up to 10 collection windows and 10
drain steps in any mode.
Up to 20 collection windows and 20
drain steps.
UniPoint control offers specific site
collection and continuous collection
options with graphical sample tracking
capabilities
Repetitive collection of same sample, or
multiple, automatic collection of
different samples.
Repetitive collection of same sample, or
multiple, automatic collection of
different samples.
Up to 99 cycles for repetitive collection
of same sample or automatic injection
of different samples.
Uses computer system monitor
2 lines, 24-alphanumeric characters
each; backlit LCD.
2 lines, 24-alphanumeric characters
each; backlit LCD.
16-alphanumeric characters plus
decimal point.
Through system controller
Inputs for start/advance and end. Event
mark output. One programmable
output for control of peripherals.
Inputs for start/advance and end. Event
mark output. One programmable
output for control of peripherals.
Nine inputs, seven outputs (including
two programmable via 25-pin connector) for injector, elution pump, recorder,
three-way valve and system shut-off.
• 17 96-well microplates
• 480 vials
• 480 tubes
(numerous configuration options
available utilizing Gilson's large
selection of standard and custom racks)
•
•
•
•
•
176 18 mm test tubes
240 12 or 13 mm test tubes
432 10 mm test tubes
4 96-well microplates
240 0.5 ml microcentrifuge tubes
without caps
• 120 0.5 ml or 1.5 ml microcentrifuge
with attached caps
•
•
•
•
•
105 13 mm test tubes
128 12 mm test tubes
108 10 mm test tubes
1 96-well microplate
60 0.5 ml or 1.5 ml microcentrifuge
tubes without caps
• 30 0.5 ml or 1.5 ml microcentrifuge
with attached caps
•
•
•
•
PC-controlled
Integrated
Integrated
Separate
Method Storage:
Method and protocol storage in
UniPoint™ System Software
Saves most recent set of
parameters.
Saves most recent set of
parameters.
Up to nine fractionation
files.
Peak Collection:
Adaptive slope algorithm accommodates
drifting baselines, negative and asymmetrical peaks, applying user-set peak
parameters. Absolute threshold level
that collects all peaks above a userspecified mV level.
Adaptive slope algorithm accommodates
drifting baselines, negative and asymmetrical peaks, applying user-set peak
parameters. Absolute threshold level
that collects all peaks above a userspecified mV level.
Adaptive slope algorithm accommodates
drifting baselines, negative and asymmetrical peaks, applying user-set peak
parameters. Absolute threshold level
that collects all peaks above a userspecified mV level.
Adaptive slope algorithm accommodates
drifting baselines, negative and asymmetrical peaks, applying user-set peak
parameters. Thirty peaks out of 99 can
be selected.
Controlled via UniPoint™ System
Software
Via Gilson Serial Input/Output Channel
(GSIOC) or by contact closure.
Via Gilson Serial Input/Output Channel
(GSIOC) or by contact closure.
Via Gilson Serial Input/Output Channel
(GSIOC) or by contact closure.
5 - 40˚ C
0 - 40° C
0 - 40° C
0 - 40° C
UniPoint™ labels the start/stop of the
fraction
100 ms pulse
100 ms pulse
210 ms pulse
± 10 or 100 mV full scale
± 10 or 100 mV full scale
± 10 or 100 mV full scale
± 10 or 100 mV full scale
cm 91.4 x 61 x 55.8
in 36 x 24 x 22
cm 47.9 x 46.4 x 33
in 18.8 x 18.3 x 13
cm 32.4 x 29.2 x 26.7
in 12.8 x 11.5 x 10.5
cm 51 x 44 x 23
in 20.1 x 17.3 x 9
Keypad controller:
cm 21.5 x 11 x 3.3
in 8.5 x 4.3 x 1.3
39.9 kg (89 lb)
11 kg (24 lb.)
5 kg (11 lb.)
8 kg (18 lb.)
Time-Based Programming:
Multi-cycle Operation:
With up to 20 collection time windows and 20 drain steps,
the 202C is a powerful fraction collection solution
Meets virtually any HPLC application requirement with its
15 operating modes
Multi-cycle operations make the 202C ideal for fraction
collection with automated, repetitive-injection HPLC
systems
Choose from six basic collection modes; time, drop, peak,
time program, signal program and manual
Expand your fraction collection capabilities by selecting
up to five racks for non-stop collection into 540 tubes
Select from nine combined modes for maximum peak
purity and sub-fractionation of selected peaks
Well suited for collection in large-scale preparative
applications
Display:
I/O Contact Closure:
Collection Vessels & Capacity:
Free vessel programming and storage of up to nine
fractionation files
Keypad Location:
202C
Computer Control:
Operating Temperature:
Event Marker:
Detector Input:
Dimensions:
Weight:
220 18 mm test tubes
300 13 mm test tubes
540 10 mm test tubes
40 user-defined vessels
Page 6
Fraction Collector Specifications
202C
High capacity, versatile,
multi-mode fraction collection
Large bed, high capacity, cold-room compatible fraction
collection with a wide variety of standard and nonstandard racks
215 NEBULA Series™
Fraction Collection
FC 204
FC 203
202C
Injection and collection
high capacity
medium capacity
versatile
Numerous flexible programming
options available through UniPoint™
System Software
Up to 10 collection windows and 10
drain steps in any mode.
Up to 10 collection windows and 10
drain steps in any mode.
Up to 20 collection windows and 20
drain steps.
UniPoint control offers specific site
collection and continuous collection
options with graphical sample tracking
capabilities
Repetitive collection of same sample, or
multiple, automatic collection of
different samples.
Repetitive collection of same sample, or
multiple, automatic collection of
different samples.
Up to 99 cycles for repetitive collection
of same sample or automatic injection
of different samples.
Uses computer system monitor
2 lines, 24-alphanumeric characters
each; backlit LCD.
2 lines, 24-alphanumeric characters
each; backlit LCD.
16-alphanumeric characters plus
decimal point.
Through system controller
Inputs for start/advance and end. Event
mark output. One programmable
output for control of peripherals.
Inputs for start/advance and end. Event
mark output. One programmable
output for control of peripherals.
Nine inputs, seven outputs (including
two programmable via 25-pin connector) for injector, elution pump, recorder,
three-way valve and system shut-off.
• 17 96-well microplates
• 480 vials
• 480 tubes
(numerous configuration options
available utilizing Gilson's large
selection of standard and custom racks)
•
•
•
•
•
176 18 mm test tubes
240 12 or 13 mm test tubes
432 10 mm test tubes
4 96-well microplates
240 0.5 ml microcentrifuge tubes
without caps
• 120 0.5 ml or 1.5 ml microcentrifuge
with attached caps
•
•
•
•
•
105 13 mm test tubes
128 12 mm test tubes
108 10 mm test tubes
1 96-well microplate
60 0.5 ml or 1.5 ml microcentrifuge
tubes without caps
• 30 0.5 ml or 1.5 ml microcentrifuge
with attached caps
•
•
•
•
PC-controlled
Integrated
Integrated
Separate
Method Storage:
Method and protocol storage in
UniPoint™ System Software
Saves most recent set of
parameters.
Saves most recent set of
parameters.
Up to nine fractionation
files.
Peak Collection:
Adaptive slope algorithm accommodates
drifting baselines, negative and asymmetrical peaks, applying user-set peak
parameters. Absolute threshold level
that collects all peaks above a userspecified mV level.
Adaptive slope algorithm accommodates
drifting baselines, negative and asymmetrical peaks, applying user-set peak
parameters. Absolute threshold level
that collects all peaks above a userspecified mV level.
Adaptive slope algorithm accommodates
drifting baselines, negative and asymmetrical peaks, applying user-set peak
parameters. Absolute threshold level
that collects all peaks above a userspecified mV level.
Adaptive slope algorithm accommodates
drifting baselines, negative and asymmetrical peaks, applying user-set peak
parameters. Thirty peaks out of 99 can
be selected.
Controlled via UniPoint™ System
Software
Via Gilson Serial Input/Output Channel
(GSIOC) or by contact closure.
Via Gilson Serial Input/Output Channel
(GSIOC) or by contact closure.
Via Gilson Serial Input/Output Channel
(GSIOC) or by contact closure.
5 - 40˚ C
0 - 40° C
0 - 40° C
0 - 40° C
UniPoint™ labels the start/stop of the
fraction
100 ms pulse
100 ms pulse
210 ms pulse
± 10 or 100 mV full scale
± 10 or 100 mV full scale
± 10 or 100 mV full scale
± 10 or 100 mV full scale
cm 91.4 x 61 x 55.8
in 36 x 24 x 22
cm 47.9 x 46.4 x 33
in 18.8 x 18.3 x 13
cm 32.4 x 29.2 x 26.7
in 12.8 x 11.5 x 10.5
cm 51 x 44 x 23
in 20.1 x 17.3 x 9
Keypad controller:
cm 21.5 x 11 x 3.3
in 8.5 x 4.3 x 1.3
39.9 kg (89 lb)
11 kg (24 lb.)
5 kg (11 lb.)
8 kg (18 lb.)
Time-Based Programming:
Multi-cycle Operation:
With up to 20 collection time windows and 20 drain steps,
the 202C is a powerful fraction collection solution
Meets virtually any HPLC application requirement with its
15 operating modes
Multi-cycle operations make the 202C ideal for fraction
collection with automated, repetitive-injection HPLC
systems
Choose from six basic collection modes; time, drop, peak,
time program, signal program and manual
Expand your fraction collection capabilities by selecting
up to five racks for non-stop collection into 540 tubes
Select from nine combined modes for maximum peak
purity and sub-fractionation of selected peaks
Well suited for collection in large-scale preparative
applications
Display:
I/O Contact Closure:
Collection Vessels & Capacity:
Free vessel programming and storage of up to nine
fractionation files
Keypad Location:
202C
Computer Control:
Operating Temperature:
Event Marker:
Detector Input:
Dimensions:
Weight:
220 18 mm test tubes
300 13 mm test tubes
540 10 mm test tubes
40 user-defined vessels
Page 6
Fraction Collection Solutions
World Wide Web: www.gilson.com
E-mail: [email protected], [email protected], [email protected]
World Headquarters
Gilson, Inc.
3000 W. Beltline Hwy., P.O. Box 620027, Middleton, WI 53562-0027, USA
Telephone: (1) 800-445-7661 or (1) 608-836-1551 • Fax: (1) 608-831-4451
Gilson S.A.
72 rue Gambetta, B.P. 45
95400 Villiers-le-Bel, France
Telephone: (33) 1-34-29-50-00 • Fax: (33) 1-34-29-50-80
303034-02, Printed in the U.S.A., February 2000, Specifications subject to change without notice
ISO 9001
Certified
Automated Fraction Collection
for HPLC or LC/MS Purification