DATA SET 1 P GENERATION Wild Type (Red Eye) x Mutant Phenotype (Sepia Eye) *no gender bias shown in F1or F2 F1 Mendelian Analysis For the Punnett Square Use (R = wildtype red) (r = Sepia) Expected Result 13 (red eye) : 0 (sepia eye) n (red eye) n (sepia eye) X2 = Pass 13 0 0 1 degree of freedom Fail F2 Mendelian Analysis Expected Result For the Punnett Square Use (R = wildtype red) (r = Sepia) 103 (red eye) : 34 (sepia) n (red eye) 106 n (sepia eye) 31 0.352 X2 = Pass Fail 1 degree of freedom DATA SET 2 P GENERATION Wild Type (Red Eye-Normal Wing) x Mutant Phenotype (Sepia Eye-Vestigial Wing) *no gender bias shown in F1 and F2 F1 Mendelian Analysis For the Punnett Square Use (R = wildtype red) (r = Sepia) (W = wildtype normal wing) (w = vestigial wing) Expected Result n (red eye-normal wing) n (red eye-vestigial wing) n (sepia eye – normal wing) n (sepia eye-vestigial wing) X2 = Pass 107 (red-normal) : 0 all other phenotypes 107 0 0 0 0 (3 degrees of freedom) Fail F2 Mendelian Analysis For the Punnett Square Use (R = wildtype red) (r = Sepia) (W = wildtype normal wing) (w = vestigial wing) Expected Result Red-normal 298: Red-vestigial 99 : Sepia-Normal 99: Sepia-Vestigial 33 n (red eye-normal wing) n (red eye-vestigial wing) n (sepia eye – normal wing) n (sepia eye-vestigial wing) 306 97 95 32 0.45 (3 degrees of freedom) X2 = Pass Fail DATA SET 3 P GENERATION Wild Type Males (Red Eye) x Mutant Phenotype Females (White Eye) *GENDER BIAS SHOWN IN F1 F1 Mendelian Analysis For the Punnett Square Use (R = wildtype red) (r = white eye) Expected Result n (red eye) n (white eye) X2 = Pass Red (17) : White (0) 17 0 0 Fail 1 degree of freedom F1 Sex-Linkage Analysis (Non-Mendelian) For the Punnett Square use XRXR or XRXr or XrXr XRY or XrY where appropriate 9 female red eyes; 8 male white eyes; 0 all other phenotypes Expected Result 9 n (red eye females) 8 n (red eye males) 0 n (white eye males) 0 n (white eye females) X2 = Pass 0 Fail F2 Mendelian Analysis For the Punnett Square Use (R = wildtype red) (r = white eye) Expected Result 185 (red) : 61 (white) n (red eye) n (white eye) 151 95 25.2 FAIL 1 degree of freedom X2 = Pass F2 Sex-Linkage Analysis (Non-Mendelian) For the Punnett Square use XRXR or XRXr or XrXr XRY or XrY where appropriate Red females/white females/white males/red males: 62 Expected Result 65 n (red eye females) 86 n (red eye males) 69 n (white eye males) 26 n (white eye females) X2 = Pass 31.13 Fail
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