Making, Mounting and Monitoring Pine Marten Hair-Tubes Hair-tubes are used to: Detect the presence of pine martens. Determine the pine martens’ genetic make-up/origin (haplotype) How they work: Sections of wastepipe erected in trees Bait is used to attract animal to enter the tube A sticky pad inside the tube collects hair from the animal that enters it The hair is DNA tested to confirm which species it came from and, if pine marten, tell us important genetic information about them They are relatively cheap to make. The current design = £2.27/tube. Component Cost Tube £1.72 Lid £0.25 Elastic strap £0.16 Bait wire <£0.01 Attachment wire <£0.05 Bait £0.05 4 x patches £0.03 RESEARCH EDUCATION CONSERVATION Charitable Company Limited by Guarantee Registered in England No. 05598716 Registered Charity No. 112100 Equipment required to make the hair-tube: Part Suggested equipment Useful tools Tube Waste pipe (110mm diam.) Handsaw Plant pot saucer for lid (115mm diam.) Jig saw Elastic cord (45cm length) Workbench/clamp Drill and drill bits (3.5mm) Measuring tape Pencil/marker pen Safety goggles Patches Corrugated plastic Chopping board Double-sided tape Stanley knife "Mouse glue" (available from www.solwayfeeders.com). Scissors Metal ruler (We can send out individuals sheets at cost price [<£2]; each sheet will make approximately 130 patches). Pen Attachment Wire (approx. 2mm diam.) Pliers (with wire-cutter) Bait attachment Wire (approx. 1mm diam.) Pliers Film canister (optional for lure) Soldering iron (optional for lure canister) RESEARCH EDUCATION CONSERVATION Charitable Company Limited by Guarantee Registered in England No. 05598716 Registered Charity No. 112100 Step-by-step guide to making a hair-tube: 1) Using the hand saw, cut a small ring from the end of the pipe. This is useful later as a stencil. 2) Measure and mark-up (37.5cm length) individual hair-tubes on the pipe. 3) Mark a strip (3cm) along the length of the pipe. 4) Cut individual tubes using the hand-saw. 5) Using the jig saw, cut out the marked 3cm section. 6) Mark out and drill six holes in each tube as shown (note that you could add a second bait hole on the other side if you wanted to add a film canister with bait inside). 7) Cut a 45cm length of elastic, feed both ends through the top hole, and tie together on inside of tube. . Step-by-step guide to making patches for hair-tube: 1) Cut rectangular patches (20mm x 25mm) from the corrugated plastic sheet. 2) Mark (10mm x 14mm) rectangles onto a mouse glue sheet. 3) Cut the sticky pads from the mouse glue sheet, using a craft knife and scissors. 4) Wrap a corrugated patch in double-sided tape. 5) Add the mouse glue patches to the corrugated patches. Backing tape Mouse glue patch Corrugated plastic 6) Wrap the patch with backing tape from the double-sided tape and secure with an elastic band. RESEARCH EDUCATION CONSERVATION Charitable Company Limited by Guarantee Registered in England No. 05598716 Registered Charity No. 112100 Step-by-step guide to mounting a hair-tube: 1) Prepare the bait. Chicken wings are recommended and these can be cut into sections to save money. 2) Get your equipment together: Hair-tubes (Tip: these can be slid inside each other to save space in your bag when carrying them) Wire (approx. 2mm. diam) to attach bait Wire (approx. 1mm. diam) to attach tube to trees Patches (prepared as above) Permanent marker pen Notepad/datasheets Pencil Pliers (with wire cutter) Lids (approx. and at least 11.5cm diam; plant pot trays work well) Bait Ribbon/ marker tie Access permit Map 3) Plan your route. 4) When you’re ready to mount your hair-tube, pull the elastic down over the tube below the top two mounting holes. 5) From the inside of the tube, feed an attachment wire through the top holes above the elastic. The length of this wire will be dependent on the girth of the tree trunk. 6) Feed a second attachment wire through the bottom holes. 7) Cut a short section (10cm) of thin wire, feed one end through the bait (around the bone if possible) and then feed both ends through the central hole in the tube. Bend the ends back to hold the bait in position. 8) Add the lid and secure it with the elastic. Elastic Lid Top attachment wire Bottom attachment wire Bait Wire securing bait RESEARCH EDUCATION CONSERVATION Charitable Company Limited by Guarantee Registered in England No. 05598716 Registered Charity No. 112100 9) Feed wire around the tree and secure by bending the ends of the wires on themselves. 10) Prepare 4 stick patches. Tip: carefully peel off the cover from the mouse glue patch and re-stick it using a tab – this makes it easier to remove when the patch is inside the tube. Mouse glue patch cover with ‘tab’ 11) Add the patches (two on the top, and two on the bottom). Tip: Align corrugations parallel to the length of the tube, and press hard! 12) Along with the bait inside the tube, you may wish to use other lures to attract a marten to the tube. Perforated cans of sardines or small film canisters containing cotton wool soaked in honey, cod liver oil or ‘marten lure’ can be hung from the tree, or nearby trees, to entice the animal(s). 13) Label the tube with a unique number, using the permanent marker pen. 14) Record information on a datasheet for each tube. 15) Take a good look around to make sure you remember where the hair-tube is! You may wish to use a ribbon or tie to identify the tree’s location from the trail, but try to make sure that the tube is not easily seen by others. RESEARCH EDUCATION CONSERVATION Charitable Company Limited by Guarantee Registered in England No. 05598716 Registered Charity No. 112100 Where to mount the hair-tubes? General location Specific location Is it somewhere a marten is likely to be? Is it practical to monitor? Were there any recent sightings in the area? Is it ‘good’ habitat? Don’t restrict yourself to pine! Time constraints Consider your route Gentle and safe terrain and accessibility Do you have the landowner’s permission? How might a marten move through the area? Linear features: Walls/streams/forest edge Animal trails Wind-blown areas (for resting sites etc) Craggy areas (den sites?) Ground cover (foraging opportunities) Tree species (consider growth) It doesn’t have to be a pine! Check tube is not damaging tree Re-check this each visit Is it in sight? Can it be easily seen by you and/or others from the right-of-way? Trade off between you/others easily finding it Consider using flagging tape/ribbon Reduce access by other species Easy to monitoring and re-bait Approximately chest height works well Height How frequently to visit the tubes: As often as you can, but this will depend on: Bait used (how quickly it will deteriorate) Time available Number of surveyors available to survey RESEARCH EDUCATION CONSERVATION Charitable Company Limited by Guarantee Registered in England No. 05598716 Registered Charity No. 112100 Step-by-step guide to monitoring hair-tubes: 1) Get your equipment together: Map (with location of tubes marked on it) Pliers Spare wire Bait Sticky patches Numbered sample bags Elastic bands A pen knife, screwdriver or bit of wire (to use as a lever to remove old patches) Data sheets Pencil First aid kit Mobile ‘phone Hair-tube with a perforated tin of sardines as a lure 2) At the hair-tube, remove the lid and check if the bait has been taken. 3) Check the sticky patches for hair samples. 4) Carefully remove the patches with hair on. A piece of wire, screwdriver or knife can be used to lever the patch off the tube. 5) Wrap the patch in backing tape, secure with an elastic band and place in a sample bag. Patches collected from the same tube can go into one bag. Tip: Backing tape from the replacement patch can be used to wrap the removed patch in. 6) Label the sample bag with the date, location, grid reference, the tube number and your initials. RESEARCH EDUCATION CONSERVATION Charitable Company Limited by Guarantee Registered in England No. 05598716 Registered Charity No. 112100 7) Check stickiness of all patches with no hair, and replace those with no/poor stickiness. 8) Replace old or eaten bait with fresh bait. 9) Record information on the data sheet. 10) Back home, enter the data from the data sheets onto a computer spreadsheet. 11) Send hair samples for DNA analysis and eagerly await the results. A marten entering a hair-tube RESEARCH EDUCATION CONSERVATION Charitable Company Limited by Guarantee Registered in England No. 05598716 Registered Charity No. 112100
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