BD Pharmingen™
Technical Data Sheet
Alexa Fluor® 647 Mouse Anti-Human IL-10Rβ (CD210b)
Product Information
564372
IL-10R2; IL-10RB; IL-10Rβ; IL10RB; IL-10 Receptor beta; CRFB4
50 tests
5 µl/test
90220
Human IL-10 Rβ Recombinant Protein
Mouse IgG1, κ
QC Testing: Human
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Material Number:
Alternate Name:
Size:
Vol. per Test:
Clone:
Immunogen:
Isotype:
Reactivity:
Storage Buffer:
Description
The 90220 monoclonal antibody specifically binds to the beta subunit (IL-10R beta/IL-10RB/IL-10Rβ) of the heteromeric IL-10 Receptor
complex. This type I transmembrane glycoprotein is also known as, subunit 2 (IL-10R2) or CD210b. The IL-10 Receptor complex is formed
by the binding of dimeric IL-10 to two cell surface alpha subunits of the IL-10 Receptor (CD210a/IL-10RA/IL-10Rα). This complex can then
recruit two CD210b subunits resulting in the transduction of intracellular JAK/STAT pathway signals. CD210b is also a component of
cytokine receptor complexes specific for IL-22, IL-26, IL-28 and IL-29. CD210b is expressed by a variety of cell types including T cells, B
cells, NK cells, monocytes and macrophages.
Multiparameter flow cytometric analysis of IL-10Rβ (CD210b) expression on human peripheral blood leucocytes. Human whole bloo
was stained with PE Mouse Anti-Human HLA-DR (Cat. No. 555812/556644/560943) and FITC Mouse Anti-Human CD14 (Cat. No.
555397/557153/561712) antibodies and either Alexa Fluor ® 647 Mouse IgG1 κ Isotype Control (Cat. No. 557714) or Alexa Fluor ® 647
Mouse Anti-Human IL-10Rβ (CD210b) antibody (Cat. No. 564372). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No.
555899). Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Panel 1. Two-parameter contour plots show the correlated expression of isotype control (Left Plot) or IL-10Rβ (CD210b) (Right Plot)
versus side-light scatter (SSC-A) characteristics for gated events with the forward light scatter properties of total viable leucocytes.
Panel 2. For gated events with the forward and side-light scatter characteristics of viable mononuclear leucocytes, the two-color contour
plots show the correlated expression of CD14 (Left Plot) or IL-10Rβ (CD210b) (Right Plot) versus HLA-DR for HLA-DR+ (and HLA-DR-)
lymphocytes and CD14+HLA-DR+ monocytes.
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Application Notes
Application
Flow cytometry
Routinely Tested
Suggested Companion Products
Catalog Number
554656
554657
557714
555812
564372 Rev. 1
Name
Stain Buffer (FBS)
Stain Buffer (BSA)
Alexa Fluor® 647 Mouse IgG1 κ Isotype Control
PE Mouse Anti-Human HLA-DR
Size
500 ml
500 ml
100 tests
100 tests
Clone
(none)
(none)
MOPC-21
G46-6
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556644
560943
555397
557153
561712
555899
PE Mouse Anti-Human HLA-DR
PE Mouse Anti-Human HLA-DR
FITC Mouse Anti-Human CD14
FITC Mouse Anti-Human CD14
FITC Mouse Anti-Human CD14
Lysing Buffer
50 tests
25 tests
100 tests
50 tests
25 tests
100 ml
G46-6
G46-6
M5E2
M5E2
M5E2
(none)
Product Notices
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental
sample (a test).
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular
Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes
(except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
7. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
8. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
9. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
www.bdbiosciences.com/colors.
References
Donnelly RP, Sheikh F, Kotenko SV, Dickensheets H. The expanded family of class II cytokines that share the IL-10 receptor-2 (IL-10R2) chain. J Leukoc Biol.
2004; 76(2):314-321. (Biology)
Koppelman B, Neefjes JJ, de Vries JE, de Waal Malefyt R. Interleukin-10
down-regulates MHC class II alphabeta peptide complexes at the plasma membrane of monocytes by affecting arrival and recycling. Immunity. 1997; (6):861-871.
(Biology)
Kotenko SV, Krause CD, Izotova LS, Pollack BP, Wu W, Pestka S. Identification and functional characterization of a second chain of the interleukin-10 receptor
complex. EMBO J. 1997; 16(19):5894-5903. (Biology)
Liu Y, Wei SH, Ho AS, de Waal Malefyt R, Moore KW. Expression cloning and characterization of a human IL-10 receptor. J Immunol. 1995; 152(4):1821-1829.
(Biology)
564372 Rev. 1
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