1268266 rev.001 Student’s Guide Name Teacher/Section Date Forensic Anthropology Objectives • Understand and implement the scientific method • Practice problem-solving skills through forensics • Perform a toxicological analysis • Perform a forensic anthropological examination to: - determine ancestry - determine age - determine stature - determine the cause of death Safety and Disposal Be sure to follow your teacher’s directions to ensure safety while conducting this laboratory investigation. Dispose of any waste materials at the end of the investigation, as directed by your teacher. Always wash your hands before leaving the laboratory. Background Toxicologists study the effects of chemical on organisms— how chemicals affect body systems, routes and symptoms of exposure, detection, and treatment. Forensic anthropology is the study of the human skeleton in a legal setting—most often in circumstances where an individual’s remains are skeletonized. Forensic anthropology is an off-shoot of the discipline of physical anthropology—the study of the human fossil record; its evolution, inheritance, adaptability and variation. Physical anthropologists (sometimes called biological anthropologists) also study other primates in addition to humans. Forensic anthropologists frequently work with other professionals—pathologists, toxicologists, odontologists (specialists who study teeth), coroners (officials who investigate deaths), homicide investigators, and other crime scene investigators to identify an individual, discover evidence of trauma, and determine when a death occurred. In the process, they routinely use many techniques of the physical anthropologist to determine age, sex, ancestry, and analyze bones for the presence of trauma and disease. You and your classmates will learn some of these techniques when you conduct your own forensic examinations. Let’s investigate the Case of the Lost Congregation. Forensic science is the body of tested knowledge obtained through the scientific method and used in a court of law to discover the truth. In these activities, you will learn the skills necessary to play the joint roles of forensic toxicologist and anthropologist. Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. Student’s Guide Name Teacher/Section Date The Case On April 5, 2007, a trench-digger was busy creating a utility trench on a land parcel in Halleck, Elko County, Nevada. A building project was underway—part of the mini tourist boom around old Fort Halleck. As the shovel began to scrap away the top layer of soil the operator thought he saw something... It looked like some bones? He stopped his machine and got off. Upon closer inspection he saw several bones, possibly a skull! The operator called the Elko County Sheriff. The Sheriff was not surprised. There were a number of ghost towns in the county. One, was a group of buildings-including a church—near the current town of Halleck—in the general vicinity of where the remains have been reported. Those buildings were still standing into the early 1920’s, but had since crumbled to dust and disappeared. It appeared that the expanding town development had finally reached the long-forgotten area. The Sheriff sent deputies and the local corner to the scene. A coroner is a public official whose responsibility is to investigate any death thought to be of other than natural causes. The county coroner, Dr. Hitchcock, took charge of the scene. All construction activity was suspended. The area cordoned off, and secured. One deputy made a quick sketch of the general area and indicated where the bones were recovered: concrete monument NORTH execation trench (see detail) fence line country road Trench Detail - position of mingled remains skull#2 single bone Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. Student’s Guide Name Teacher/Section Date Dr. Hitchcock made a phone call to the State Historical Society to ask for a report on the old group of buildings known to have been in the vicinity. He particularly wanted to know if the church had a cemetery. He also wanted to know if there were any recorded military or Native American burials in the vicinity. What about old Fort Halleck? Surely it had a military cemetery? Perhaps these were the remains of interred soldiers? • Can any additional information concerning these remains be gleaned from the examination of the bone material; from the detailed anatomical drawings? Forensic technicians have taken a soil sample around skull #1 for later analysis by your team. A soil analysis would provide information about whether any heavy metals or other poisons would have leached out the bones. These two compounds are often connected with the possibility of foul play or inadvertent poisoning. Both are naturallyoccurring elements that can be picked up by miners. There certainly was no shortage of miner graves in Nevada, the silver state. Dr Hitchcock would appreciate a complete and documented report. The coroner was pretty sure that they had uncovered a historic archeological site, not a crime scene. But one could never be too sure... Dr. Hitchcock wants the soil sample tested for arsenic and lead. He has directed that the co-mingled bones be recovered. He has called on the services of a forensic anatomical artist to create an accurate set of drawings (front and side views) of each of the skulls and a bone fragment. These artifacts are too delicate to be handled extensively. The coroner has directed that the recovered bones, along with the five drawings, be made available to your team for a forensic analysis. Dr. Hitchcock would like your team’s findings concerning: • Lab results from the processed soil sample connected with the recovered bones. The coroner would like a soil analysis for the presence of the heavy metal (lead) and arsenic. • Are the recovered remains human? • How many separate individuals can be identified from the co-mingled remains? • Can the ancestry, gender, and stature be obtained and possibly be compared with other historical information to determine if these remains are involved with a wrongful death? Nevada State Historical Society Report to Dr. Hitchcock: Dr. Hitchcock: The area where human remains were found is near Fort Halleck in Elko County, Nevada. There were two other structures known to be in the immediate area—a school house (1872 – 1886), and a church (St. Casimar; 1875 – 1890). The church also had a small cemetery. No birth or death certificates are available in Nevada before 1887 when the state determined that these types of records must be kept. The only sources that might have information are the early newspapers, sexton (graveyard custodian) records or baptismal records that may be on file at various churches in the state or from tombstones if they exist. I have had my staff check our achieves, and we have found a sexton’s Burial Record book for St. Casimar church which was located eight miles outside the present town of Halleck, NV. The Nevada State Department of Records has no information about the location of burial plots for the church cemetery. The sexton made a crude drawing on the front page of the Record Book. I have also included some background information on Fort Halleck: First named Camp Halleck, after its commander, US Army major general Henry Halleck in 1867, it was renamed Fort Halleck in 1879. The fort was established to protect those who were traveling on the California Emigrant trail and building Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. Student’s Guide Name Teacher/Section Date the Central Pacific Railroad. The Fort was approximately 12 miles from the town of Halleck, Nevada and the Central Pacific railroad. Washington exerted pressure to move the Fort closer to the railhead, but it never happened mainly because, at the time, Fort Halleck was the most expensive fort then constructed by the Army! Since its location was removed from the lines of commerce, Fort Halleck was abandoned in October of 1886. Later, the Department of the Army had the military graves moved to the San Francisco National Cemetery. We believe that your discovery may be the co-mingled remains of one or more burials from St. Casimar. Yours truly, Mathew Duncan Chairman Parker Burial March, 12, 1883; Grave 4 Infant Leonard and Anna Isaac Harmon Burial July 25, 1883; Grave 5 He had come to NV in 1872 and had resided in and about Ely for the last eleven years prospecting and mining. Also prepared game trophies for hunters. Nicknamed “Captain.” Served during the civil war as a pvt in Co. B, 13th Maine Vol., and later as a 1st Lt. in the 80th U.S. Colored Cavalry. Hong Lee Burial April 5, 1884 Grave 6. Laborer, Central Pacific Railroad. Vry small-sized man. Aged 35. Sarah Connors Burial May 15, 1886; Grave 7 Cook at for until sickness. Aged 49. Consumption. Burial Record St. Casimar Church Elko, Nevada 1875 - 1 James Johnson, Sexton 2 Charles Bagsby Burial October 18, 1877; Grave 1 Blacksmith and miner. Served in the 45 ILL Infantry in the Great War. Aged 56 years. George Clark Burial July 24, 1878; Grave 2 Prospector. Body found in a barren gulch some 8 miles from his cabin at Red Hills between Red Hills and the Boundy Brothers Ranch. Aged 43. Arthur Cahill Burial November 20, 1882; Grave 3 U.S. Marshall. Bushwacked. Lived In Virginia City. Aged 50 years. Jordan Banks to hang for murder in Carson City. 3 4 5 8 7 Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 6 Student’s Guide Name Teacher/Section Date Soon the elder Blandy grew ill - first stomach distress, then sweats and growing weakness. Growing suspicious that he was being poisoned; Blandy asked an apothecary (pharmacist) to examine his food. He wasn’t sure, but it could be arsenic…. Activity 1 Toxicological Investigation of Soil Background For some strange reason, Blandy continued to allow his daughter to prepare his food. And for not so strange a reason, he died. Cranstoun fled, but Mary was caught and captured. She was placed on trial in 1752. Four physicians testified that they had looked at Blandys internal organs at autopsy and that arsenic had killed him. They said that the liver and other organs had a preserved quality to them— indicative of arsenic poisoning. Mary was found guilty. She was hanged on April 6, 1752. Toxicology—the first forensic science Toxicology is the study of the adverse effects of chemical, physical, or biological agents on living organisms. In forensics, poisons are a popular method for murder. Almost any natural substance in the appropriate amount or concentration can be injurious. Many poisons mimic common medical diseases leading investigators to assume that the death was from natural causes. Toxicology is not only important in investigating whether foul play occurred, but also in other circumstances such as suspected suicide, accidental death, and substance abuse. Arsenic Poisoning The history of forensic toxicology goes back over 200 years. One of the first trials involving toxicological evidence and testimony occurred in 1752. A certain Captain William Cranstoun proposed marriage to Mary Blandy. Captain Cranstoun was an unscrupulous fellow in debt—he hatched a plan to get Mary’s father’s estate. Cranstoun told Mary that he knew an herbalist who might help them settle her father’s estate “on both of them.” The captain produced a white powder and told Mary to add it to the old man’s tea—in small doses. Months passed, and Mary could see no signs of distress in her father. So she began to add the white powder directly to his food. Arsenic (symbol: As; atomic number 33) is a chemical element. It has many forms: yellow (molecular non-metallic) and several black and gray forms (metalloids). It is more commonly found as arsenide and arsenate compounds. For centuries, arsenic has been known—and used—as a poison. Its symptoms mimic those of cholera—gastrointestinal tract upset, stomach cramps, and massive watery diarrhea. Arsenic has been used as an insecticide and preservative— in early embalming fluids, wood preservatives, and by taxidermists. A forensic test for arsenic was developed by chemist James Marsh in 1836. The Marsh test for arsenic trioxide (As2O3)— a white powder easily incorporated into food and drink. A blue color indicates a positive test. Before the Marsh test, arsenic was undetectable in the body. Lead Poisoning Lead (symbol: Pb; atomic number 82) is a chemical element. Lead is bluish white when freshly cut, but turns to dull gray when exposed to the air. Lead has the highest atomic number of all the stable elements. Like mercury, and other heavy metals, lead can accumulate over time in body tissues and bone. Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. Student’s Guide Name Teacher/Section Date Lead is usually found in ore with zinc, silver, and copper. It is extracted together with these metals. The main lead mineral is galena (PbS), which contains 86.6% lead. Lead mining ranked as Nevada’s second-richest mineral producer, behind silver. Lead mining in the state peaked in 1878, and declined thereafter due to decreasing mine production and changing market conditions. Lead is all around us! It has routinely been added to paint as pigment for centuries—Lead (II) chromate (PbCrO4, chrome yellow) and lead (II) carbonate (PbCO3, white lead) being the most common forms. These additives were incorporated into paint formulas to speed drying, increase durability, retain a fresh appearance and resist moisture. Today, paint containing more than 0.06% lead is banned for residential use. Lead poisoning is a medical condition caused by increased blood lead levels (BLL). BLL measures current exposure to lead, but lead is also incorporated into bone from prior exposures. X-ray metering can measure bone-lead levels. Symptoms of chronic lead poisoning include neurological and gastrointestinal problems, excess lethargy or hyperactivity, and, in extreme cases, seizure, madness (dementia), coma, and death. Today, routine lead testing is done on soils to check for the presence of lead salts. The presence of a pink-red color in the test result indicates the presence of free lead. What You Need... Per Student - Activity 1 – Student sheets - safety goggles Per Group 1 piece cheesecloth, 4x4” 2 medicine cups, empty 2 medicine cups, each filled with 20 mL extraction solvent 2 paper cones 2 weighing dishes, 8 cm 1 EVIDENCE envelope containing the soil sample 1 teaspoons, plastic Per Class Accidental lead poisoning was believed responsible to the crew of 128 from the Franklin, a British expedition to the Arctic in 1845. The new-technology of canned food on board was determined to have contributed to the crew’s demise—all the tin cans were sealed with lead solder. The lead is thought to have leached into the food and longterm consumption of contaminated food eventually driving the men mad. 1 balance Many historians believe the music composer Ludwig van Beethoven suffered from lead poisoning. X-ray tests done in 2005 at the Argonne National Laboratory on fragments of Beethoven’s skull have confirmed clinically-significant levels of lead. If the toxicological analysis is positive, the Coroner would like a written report detailing: • How the chain of custody was protected • A description of the soil extraction process • The reagents applied • Test results and findings Overview Dr. Hitchcock would like the submitted soil sample analyzed for the presence of lead and/or arsenic. Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. Student’s Guide Name Teacher/Section Date Protecting the Chain of Custody The chain of custody refers to a document trail showing the acquisition, custody, control, transfer, analysis, and disposition of physical evidence. Physical evidence must be handled in a careful manner to avoid charges of tampering or falsification. A specific individual must always have the physical custody of a piece of evidence. What to do paper cone medicine cup STEP 4 Fold the cheesecloth into a 1-inch-sqaure, and place in the bottom of the paper cone. The cheesecloth will act as a barrier for soil particles. STEP 1 paper cone Record the information on the EVIDENCE envelope on your Activity sheet (Evidence Intake Information) to preserve the chain of custody of the soil evidence. medicine cup cheesecloth Case No. Lab Investigator Date & Time of Recovery Weighing the Soil Sample Scene Location Item Description Place a weigh dish on a balance and determine its tare weight. Record this value on the Laboratory Data portion of the Activity sheet. STEP 2 Remember that your team will need to prepare two soil extraction samples—one for each substance being analyzed. Open the EVIDENCE envelope, and remove the sealed sample. Record information about the sample on the Evidence Intake Information portion of the Activity sheet. Use a balance to weigh the sample (soil sample in zip-closure bag) so that it can adequately be described— e.g. approximately 125 grams of collected soil. STEP 3 Test fit the paper cone into in an empty medicine cup. The paper cone should fit snugly as illustrated: STEP 5 Gently pour the collected spoonful of soil onto the tare’d weigh dish and weigh it on the balance. Determine the sample weight (mass) by subtracting the tare weight from the total sample weight to obtain the net sample weight. Record this value on the Laboratory Data portion of the Activity sheet. STEP 6 Transfer the weighed sample from the balance and folding it so that the soil can be poured into the paper cone. Remember to pour the soil sample into the middle of the cheesecloth barrier. Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. Student’s Guide Name Teacher/Section Date STEP 7 STEP 10 Your team will now extract any heavy metals and/or arsenic trioxide that may be present from the collected soil into the lower medicine cup by carefully pouring the extraction solvent over the soil sample in the cup. Allow the poured liquid to drain completely through the soil sample. Remember to record the exact volume of the extraction solvent on the Laboratory Data portion of the Activity sheet. Repeat Steps 3 through 9 for the other sample; testing for the presence of lead. A pink-red color is indicative of a positive test for the presence of lead. At the conclusion of the test, record your team’s test results on the Laboratory Data portion of the Activity sheet. STEP 8 STEP 11 Clean up your area as your teacher directs. Wash your hands before leaving the laboratory. Once the extraction solvent has completely drained through the soil sample, remove the upper paper cone and soil. Discard the extracted soil sample as your teacher directs. STEP 9 Note: Put on protective goggles. Perform the Arsenic Test (Marsh Test for arsenic trioxide As2O2) by applying 5 drops of the test reagent to the extracted solvent sample. A blue color indicates the presence of arsenic trioxide. Record the test results on the Laboratory Data portion of the Activity sheet. Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. Student’s Guide Name Teacher/Section Date County Crime Laboratory EVIDENCE INTAKE INFORMATION: Date: Chain of Custody: From: Comments: County Sheriff’s Office Halleck, Elko County, Nevada Description: Description of Sample: To: Date: LABORATORY DATA Sample No. Tare Weight: grams Net soil sample weight: grams Arsenic Test: Marsh Reagent: (Volume of extraction solvent used: mL) Test Results: Sample No. Tare Weight: grams Net soil sample weight: grams Lead Test: Lead Reagent: (Volume of extraction solvent used: mL) Test Results: Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. Student’s Guide Name Teacher/Section Date Analysis & Findings 3. Has a crime been committed? Was a poisonous substance found in the collected soil sample(s)? 4. W hat other issues do the findings suggest? Questions 1. What is toxicology? 5. W hat other tests could be run? 2. Is the presence of either lead or arsenic associated with the recovered bones a significant finding? Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 10 Student’s Guide Name Teacher/Section Date Activity 2 Head Forensic Identification of Recovered Co-mingled Remains – Elko County, Nevada Background Note: Use the Forensic Anthropology Guide to learn more about terminology, bone and skeletal anatomy. Forensic anthropologists perform an evaluation and analysis on either a complete or partial skeleton. Sometimes the recovered remains are co-mingled—with bones of two or more individual found together. skull un-paired bone paired bone Foot Findings: What to do STEP 1 Determining the Number of Individual in Co-Mingled Remains The first task is to determine if the remains are commingled or separate. This involves laying out the bones in the correct anatomical position on a flat surface, such as a table. Individual bones are identified: paired bones (right and left) and non-paired bones. For example, having three scapulas immediately tells you that you are dealing with two individual skeletons. Two skulls (labeled Skull #1 and Skull #2) and a femur have been retained by the coroner. He has had a set of accurate medical illustrations made that your team can use for its forensic analysis. Record the symbol and the identity of the bone (or skull) on the orientation diagram below. STEP 2 Determining Relative Age, Trauma, and Presence of Disease Next, each bone is examined for growth status, trauma, and disease. Use the Forensic Anthropology Guide as a guide to skeletal landmarks. Record your data on the Skeleton Inventory sheet. Record your summary findings below: Are there any signs of epiphyseal plate closure on the long bones indicating that the bone is from an adult (see Epiphyseal Union Table) aged greater 25 years? Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 11 Student’s Guide Name Teacher/Section Date Epiphyseal Union Table Long Bone Male Female humerus (head) 20-25 yrs. 18-22 yrs. humerus (condyle) 14-18 yrs. 14-17 yrs. radius / ulna 17-20 yrs. 16-19 yrs. femur (head) 18-21 yrs. 16-19 yrs. femur (condyle) 17-20 yrs. 16-19 yrs. tibia / fibula 17-20 yrs. 16-19 yrs. Adapted from: Brothwell, D.R. 1981. Diggin Up Bones: The Excavation, Treatment and study of human skeletal remains. Cornell University Press, Ithaca, New York. Are there any signs of trauma? Are there any signs of disease? - Does the bone have a sandpapered appearance where usual surface detail appears to be obliterated? - Are there discolorations? Immature Skeletons: mandible not fused (birth to 12 months) appearance of ossification growth centers (birth to 15 years) Mature Skeletons: bone growth centers (epiphyseal plates) fused in long bones? yes no sub adult (<25 years) adult (> 25 years) adult (> 50 years) STEP 3 Record all information regarding each bone on the Skeleton Inventory Form for all recovered bones and bone fragments: Record your answers on the Skeleton Inventory sheet. Findings: Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 12 Student’s Guide Name Teacher/Section Date Skeleton Inventory Form Date: Case No. Examiner: Elko County Nevada Crime Lab Skull #1 Orbital Rim Landmarks Notes Rounded Not Rounded Projects below base of skull Does not project below base of skull Cranial Breadth [euryon (eu) – euryon (eu)] (mm) Cranial Height [glabella (g) – opistocranium (op)] (mm) Calculations: Facial Height [nasion (n) – gnathion (gn)] (mm) Facial Width [zygion (zy) – zygion (zy)] (mm) Calculations: Nasal Breadth [alare (al) – alare (al)] (mm) Nasal Height [nasion (n) – nasiospinale (ns)] (mm) Calculations: Notes Eye Orbit Shape Mastoid Process Gonial angle Mandible (front profile) Cranial Index Facial Index Facial Index Facial Profile Type Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 13 Student’s Guide Name Teacher/Section Date Skeleton Inventory Form Date: Case No. Examiner: Elko County Nevada Crime Lab Skull #2 Orbital Rim Landmarks Notes Rounded Not Rounded Projects below base of skull Does not project below base of skull Cranial Breadth [euryon (eu) – euryon (eu)] (mm) Cranial Height [glabella (g) – opistocranium (op)] (mm) Calculations: Facial Height [nasion (n) – gnathion (gn)] (mm) Facial Width [zygion (zy) – zygion (zy)] (mm) Calculations: Nasal Breadth [alare (al) – alare (al)] (mm) Nasal Height [nasion (n) – nasiospinale (ns)] (mm) Calculations: Notes Eye Orbit Shape Mastoid Process Gonial angle Mandible (front profile) Cranial Index Facial Index Facial Index Facial Profile Type Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 14 Student’s Guide Name Teacher/Section Date Appendicular Skeleton Bone Side Percent Complete Condition Osteometric Measurement (mm) Upper Limbs Clavicle L R Scapula L Humerus Radius Ulna L: L: W: R L R L R L: L: L: L: L: L R Hands Carpals Metacarpals Phalanges __ of 16 Lower Limbs __ of 28 __ of 10 L Os Coxa R L Femur R L L: HD: Shaft Diameter: L: HD: Shaft Diameter: L: L: Fibula R L: Patella L R L: L: R Tibia L Feet Tarsals Metatarsals Phalanges R __ of 14 __ of 10 Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 15 Student’s Guide Name Teacher/Section Date Thorax – Spinal Column Bone Side Percent Complete Condition Osteometric Measurement (mm) Cervical atlas axis C3-C7 Thoracic T1-T12 Lumbar L1-L5 Sacrum S1-S5 Coccyx L: W: coccyx Sternum manubrium sternum Body xiphoid Ribs L 1-12 R Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 16 Student’s Guide Name Teacher/Section Date STEP 4 Determining Ancestry Determine ancestry using cranial traits. Use the Forensic Anthropology Guide as a guide to skeletal landmarks. Record your osteometric (bone measurement) data on the Skeleton Inventory sheet. Use the Human Ancestral Traits Table to determine likely ancestry. Record your summary findings below: (G) Mandible—chin: Place the mandible in side profile on a table. Examine the relative projection of the chin. Summary Findings Skull #1 Ancestry Determination: - face profile: (A) Calculate the cranial index. - eye orbit shape: (B) Calculate the facial index. - gonial angle: - chin profile: (C) Calculate the nasal index. Skull #2 (D) Determine the facial profile: (E) Eye orbit shape: Examine the skull face-on. Describe the overall shape as square or rounded. If the eye orbits are rounded, examine the upper border. Is it level, or does it slope laterally? (F) Mandible (jaw)—Gonial Angle Place the mandible in anterior (front) view directly in front of you. Examine the gonial angle to see if it is rounded. Ancestry Determination: - face profile: - eye orbit shape: - gonial angle: - chin profile: Human Ancestral Traits Cranial Trait European Descent African Descent Asian Descent (A) Cranial Index 75 to 80 <75 >80 (B) Facial Index >90 (narrow to very narrow) < 85 (broad to very broad) 85 to 90 (medium) (C) Nasal Index <48 (narrow) > 53 (wide) 48 to 53 (intermediate) (D) face profile orthognathic (straight, flat) prognathic (projecting-upper teeth socket area) intermediate to mostly orthognathic (E) eye orbit shape rounded and sloping square or rectangular rounded and non-sloping (F) gonial angle rounded not rounded rounded (G) chin profile prominent and projecting rounded slightly projecting Adapted from: Bass, 1995; White, 2000 Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 17 Student’s Guide Name Teacher/Section Date STEP 5 Limb Lengths Related to Sex Determining Gender Use the Forensic Anthropology Guide as a guide to skeletal landmarks. Record your data on the Skeleton Inventory sheet. Record your summary findings below: Background Humans vary (about 12%) in their degree of sexual dimorphism—differences in form between males and females—mostly in body size, robustness, muscularity, and teeth size. Sexual dimorphism can be used to determine the sex of unknown individuals. In humans, although other bones are used, the skull, long bones, and pelvis are primarily used to determine gender. Pelvis Male Female greater sciatic notch narrow angle; relatively deep wide angle; relatively shallow subpubic angle under 90°; narrow generally 90° or higher Skull Male Female orbital rim rounded not rounded mastoid process medium to large (thumb-size), usually projecting below the base of the skull small (little finger size), usually does not project below the base of the skull mandible heavier jaw, more square chin that has two points connected by more or less a straight line. More narrow and pointed Bone Female Intermediate Male Femur (maximum length) 412.0 432.5 453 Humerus (maximum length) 303 313.5 324 Os Coxae obturator foramen Small and triangular --- Large and ovoid Os Coxae sciatic notch Wide; > 90 degrees --- Narrow; < 90 degrees Os Coxae acetabulum small --- large Scapula (maximum length) 129 137.5 146.1 Scapula (maximum width) 88.1 92.7 97.3 Clavicle (maximum length) 125.3 132.5 139.6 Radius (maximum length) 231.5 242.1 252.7 Ulna (maximum length) 247.9 258.9 269.9 Tibia (maximum length) 355.0 366.7 378.5 Adapted from: White, T.D. 1991. Human Osteology. Academic Press, San Diego, CA. Note: data in mm; left side (standard) Summary Findings: Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 18 Student’s Guide Name Teacher/Section Date STEP 6 Determining Stature Use the Forensic Anthropology Guide as a guide to skeletal landmarks. Record your data on the Skeleton Inventory sheet. Record your summary findings below: Background The height or stature of an individual is useful in making forensic identification, inclusion, and exclusion determinations. Before estimating stature, one must first determine the ancestry, gender, and age of an individual (if possible) since stature varies within these variables. Note: Stature determinations are estimates, never exact. Stature estimates are calculated in centimeters (cm). An individual who is 5 feet, 6-inches tall is 167.6 cm tall. (5.5ft x 12 in/ft x 2.54 cm/in = 167.64cm) Stature is estimated by comparing measurement data of certain bones to tables of published data. The most commonly used bones for stature estimation for incomplete remains are the long bones (femur, tibia, fibula, humerus, radius, ulna), but short bones of the hands and feet may also be used. It is important to remember that most individuals thirty years of age, and older, have undergone some degree of degenerative change associated with the aging process which results in a decrease in stature. To account for this loss of height associated with aging, one must subtract from the stature estimates. The estimated age is plugged into an equation; the answer is then subtracted from the stature estimate. Determine the stature of an individual by inserting the appropriate maximum length measurement for any long bone in the table below. If ancestry and gender is known, use the appropriate formula. If either gender or ancestry is not known, use the default formula. Example The estimate range in stature of an individual of known African decent whose femur measures a maximum length of 447.3 mm would be calculated as: 447.3 mm = 44.7 cm 2.10 x 44.7 cm = 98.78 + 72.22 = 171.00 cm 171.0 cm + 3.94 cm = 174.94 cm / 2.54 cm/inch = 68.87 inches / 12 in/foot = 5 foot 9 inches 171.0 cm – 3.94 cm = 167.06 cm / 2.54 cm/inch = 65.77 inches / 12 in/foot = 5 feet 6 inches Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 19 Student’s Guide Name Teacher/Section Date Stature Table Bone Femur Race Male Data Female Data European Descent 2.32 x femur length + 65.53 ± 3.94 2.47 x femur length + 54.10 ± 3.72 African Descent 2.10 x femur length + 72.22 ± 3.91 2.28 x femur length + 59.76 ± 3.41 Asian Descent 2.15 x femur length + 72.57 ± 3.80 data not available Default Tibia European Descent 2.42 x tibia length + 81.93 ± 4.00 2.90 x tibia length + 61.53 ± 3.66 African Descent 2.19 x tibia length + 85.36 ± 3.96 2.45 x tibia length + 72.56 ± 3.70 Asian Descent 2.39 x tibia length + 81.45 ± 3.24 data not available Default Fibula 2.60 x fibula length + 75.50 ± 3.86 2.93 x fibula length + 59.61 ± 3.57 African Descent 2.34 x fibula length + 80.07 ± 4.02 2.49 x fibula length + 70.90 ± 3.80 Asian Descent 2.40 x fibula length + 80.56 ± 3.24 data not available 2.89 x humerus length + 75.48 ± 4.57 3.36 x humerus length + 57.97 ± 4.45 African Descent 2.88 x humerus length + 78.10 ± 4.57 3.08 x humerus length + 64.67 ± 4.25 Asian Descent 2.68 x humerus length + 83.19 ± 4.16 data not available 3.01 x humerus length + 71.88 ± 4.40 European Descent 3.76 x ulna length + 75.55 ± 4.72 4.27 x ulna length + 57.76 ± 4.30 African Descent 3.20 x ulna length + 82.77 ± 4.74 3.31 x ulna length + 75.38 ± 4.83 Asian Descent 3.48 x ulna length + 77.45 ± 4.66 data not available Default Radius 2.55 x fibula length + 73.33 ± 3.70 European Descent Default Ulna 2.33 x tibia length + 82.91 ± 3.73 European Descent Default Humerus 2.26 x femur length + 64.83 ± 3.76 3.60 x ulna length + 73.78 ± 4.65 European Descent 3.79 x radius length + 79.42 ± 4.66 4.74 x radius length + 54.93 ± 4.24 African Descent 3.32 x radius length + 85.43 ± 4.57 3.67 x radius length + 77.79 ± 4.59 Asian Descent 3.54 x radius length + 82.00 ± 4.60 data not available Default 3.81 x radius length + 75.91 ± 4.53 Data adapted from: Bass, W.M. 1995 Human Osteology: A Laboratory and Field Manual, 4th Edition. Missouri Archaeology. Note: data in cm Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 20 Student’s Guide Name Teacher/Section Date Stature calculations Summary Findings: Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 21 Student’s Guide Name Teacher/Section Date STEP 7 Site Drawing Compare the summary osteometric data with historical data provided by the Nevada Historical Society. What are the findings in this case? 1 Summary Findings: 2 3 4 5 8 7 Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 6 22 Student’s Guide Name Teacher/Section Date Report to Dr. Hitchcock: Note: The names of individuals and case scenarios are fictitious. Any semblance to actual persons, crimes, or locations is entirely coincidental. Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 23 Student’s Guide Name Teacher/Section Date 2. Use the library and internet (with permission) to research the following topics: Going Further 1. See if you can analyze the following osteometric data and identify the remains found in a shallow grave: Femur #1 Femur #2 Humerus #1 Humerus #2 maximum length maximum length maximum length maximum length 52.5cm 42.4 cm 30.0 cm 37.4 cm Height measurements for the following Europeandescendant individuals are known: Caroline 5’ 3 ½ “ Bob 6’ 2” Clarence 5’ 8 ¾ “ Gretchen 5’ 8” - The Body Farm at the University of Tennessee. - United States Central Identification laboratory (CIL) is the only facility of its type in the world. It has responsibility for the identification of United States military war dead www.jpac.pacom.mil/CIL/CIL_Home.htm - Research Neanderthal long bone measurement data. How does the stature of a Neanderthal compare with Homo sapiens? 3. Using the Glossary in the Forensic Anthropology Guide, see how many bone structures you can observe on human bones. Calculations Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 24 Student’s Guide Name Teacher/Section Date Neat Websites Houde, John. Crime Lab: A Guide for Nonscientists. Calico Pr., 1998. Careers in Forensic Science: www.forensicdna.com/careers.htm Kessler, Ronald and P. McCarthy (Editor). The FBI/Inside the World’s Most Powerful Law Enforcement Agency. New York: Pocket, 1994. Federal Bureau of Investigation www.fbi.gov/homepage.htm Morgan, Marilyn. Careers in Criminology. McGraw-Hill, 2000. Facial Imagery – FBI / Smithsonian Collaboration www.fbi.gov/hq/lab/fsc/backissu/oct2000/ubelaker. htm Massie, Robert K. The last Romanov Mystery. The New Yorker. Pages 71-95. August 21 & 28, 1995. Royal Canadian Mounted Police www.rcmp.ca/ American Academy of Forensic Sciences www.aafs.org Rainis, Kenneth G. Exploring With a Magnifying Glass. New York: Watts, 1996. Rainis, Kenneth G. Crime-Solving Science Projects. Forensic Science Experiments, Berkeley Heights, New Jersey: Enslow, 2000. Combined DNA Index System (CODIS) http://www.fbi.gov/hq/lab/codis/index1.htm Rainis, Kenneth G. Microscope Science Projects and Experiments—Magnifying the Hidden World. Berkley Heights, New Jersey: Enslow, 2003. New Scotland Yard www.met.police.uk/ Rainis, Kenneth G. Blood & DNA Crime-Solving Science Projects. Berkley Heights, New Jersey, Enslow, 2006. PBS (Public Broadcasting Service) – History Detectives www.pbs.org/opb/historydetectives/techniques/ forensic_ml.html Ramsland, Katherine. The Forensic Science of C.S.I. San Francisco: Berkley Publishing Group, 2001. Learn and Read More About It Barber, Jacqueline. Crime Lab Chemistry: Teacher’s Guide. Berkley. Gems; Lawrence Hall of Science, 1993. Camenson, Blythe. Opportunities in Forensic Science Careers. VGM Career Books: Chicago, 2001. Sillitoe, Linda et al. Salamander: The Story of the Mormon Forgery Murders. New York: Signature, 1990. Toobin, Jeffrey. The CSI Effect. New Yorker. Pages 30-35. May 7, 2007. Wiese, James and E. Shems (Illustrator). Detective Science: 40 Crime-Solving, Case-Breaking, Crook-Catching Activities for Kids. New York: Wiley, 1996. Evan, Colin. The Casebook of Forensic Detection: How Science Solved 100 of the World’s Most baffling Crimes. New York: Wiley, 1998. Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 25 Student’s Guide Name Teacher/Section Date Evidence Skull #2 Case No 23-1234 Accurate illustration of recovered Skull #2 from Elko County, Nevada Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 26 Student’s Guide Name Teacher/Section Date Evidence Case No 23-1234 Accurate illustration of recovered skull #2 from Elko County, Nevada Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 27 Student’s Guide Name Teacher/Section Date Evidence Skull #1 Case No 23-1234 Accurate illustration of recovered Skull #1 from Elko County, Nevada Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 28 Student’s Guide Name Teacher/Section Date Evidence Case No 23-1234 Accurate illustration of recovered Skull #1 from Elko County, Nevada Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 29 Student’s Guide Name Teacher/Section Date Evidence Case No 23-1234 Accurate illustration of recovered proximal right femur from Elko County, Nevada Talking Bones COPYMASTER: Permission granted to make unlimited copies. Copy use confined to educational purposes within a single school building. Copyright © Delta Education LLC. A member of the School Specialty Family. All rights reserved. 30
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