Arthropod Management Tests 2014, Vol. 39 doi: 10.4182/amt.2014.G10 (G10) TURFGRASS: Tall fescue, Schedonorus arundinaceus (Schreb.); Kentucky blue grass, Poa pratensis L., and perennial rye grass, Lolium perenne L. EVALUATION OF INSECTICIDE APPLICATION TIMINGS AGAINST WHITE GRUBS IN COOL SEASON TURFGRASS IN VIRGINIA, 2013 Sudan Gyawaly Department of Entomology Virginia Tech Blacksburg, VA 24061-0319 Phone: (681) 285-5905 Email: [email protected] R. R. Youngman C. A. Laub T. P. Kuhar White grubs (WG): Cyclocephala lurida Bland and Popillia japonica Newman This study was conducted to examine the efficacy of two rates of insecticides and timing of application against white grubs in cool season turfgrass in Virginia. Treatments included low and high recommended rates of Merit (imidacloprid) and Acelepryn (chlorantraniliprole) and an untreated check. Insecticides were applied as spring, summer, early fall and late fall applications. Experiments were conducted at two locations: Virginia Tech Turfgrass Research Center, Blacksburg, VA, and Tazewell County Country Club, Tazewell, VA. Turf plots 5 ft (1.5 m) by 5 ft (1.5 m) were used as experimental units. Treatments were assigned to plots in a RCB with four replicates. Insecticides were applied as foliar sprays on 1 May (spring), 25 Jul (summer), 11 Sep (early fall), or 3 Oct (late fall) in Blacksburg and on 24 Apr (spring), 11 Jul (summer), 10 Sep (early fall) or 1 Oct (late fall) in Tazewell using a CO2 backpack sprayer. The backpack sprayer was equipped with 4,8002VS stainless steel spray tips and calibrated to deliver 20 gpa at 40 psi. On 5 Nov in Blacksburg and 31 Oct in Tazewell, a 1 ft² sample of turf from the center of each plot was cut and the number of live WG recorded. Treatment means were compared using ANOVA. Means were separated using Student’s t at the 0.05 level of significance. All data were subjected to square root transformation to normalize variance, but untransformed means are presented. WG density was relatively low at both locations, and was comprised of predominately southern masked chafer (85% of the total WG) at Blacksburg and Japanese beetle (70 % of the total WG) at Tazewell. There was a significant effect of treatment at both locations. In general, all the insecticide treatments applied as spring or summer applications resulted in relatively fewer numbers of grubs compared to early fall or fall applications of the same insecticide treatments. However, none of the treatments had significantly lower numbers than control treated plots at both locations. No phytotoxicity was observed from any of the insecticide treatments. This experiment was supported by products and research funding from Dupont. 1 Arthropod Management Tests 2014, Vol. 39 doi: 10.4182/amt.2014.G10 Table 1. Blacksburg Treatment formulation Rate Application Density of WG/ft² (oz) /acre timing on 5 Nov Acelepryn 1.67SC 16.0 Acelepryn 1.67SC 8.0 Merit 75WP 8.6 Merit 75WP 4.3 Untreated check --- 1 May 25 Jul 11 Sep 3 Oct 1 May 25 Jul 11 Sep 3 Oct 1 May 25 Jul 11 Sep 3 Oct 1 May 25 Jul 11 Sep 3 Oct 1 May 1.75abc 0.00c 0.50c 0.00c 0.00c 0.00c 1.25abc 2.75ab 0.00c 0.00c 1.25abc 3.25a 1.75abc 0.00c 2.00abc 1.25abc 1.12bc Means within each column followed by the same lowercase letter are not significantly different according to Student’s t (P>0.05). Table 2. Tazewel Treatment formulation Rate (oz) /acre Acelepryn 1.67SC 16.0 Acelepryn 1.67SC 8.0 Merit 75WP 8.6 Merit 75WP 4.3 Untreated check --- Application Density of WG/ft² timing on 31 Oct 24 Apr 11 Jul 10 Sep 1 Oct 1 May 25 Jul 11 Sep 3 Oct 1 May 25 Jul 11 Sep 3 Oct 1 May 25 Jul 11 Sep 3 Oct 24 Apr 0.00d 0.00d 4.50abc 4.00abcd 0.50cd 0.25cd 1.00cd 4.00abcd 0.75cd 0.75cd 5.75ab 8.25a 1.50bcd 0.75cd 1.00cd 5.75ab 4.00abcd Means within each column followed by the same lowercase letter are not significantly different according to Student’s t (P>0.05). 2
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