Enhancing Conservation and
sustainable use of
Dalbergia and
Shorea spp.
Suchitra Changtragoon
Department of National Parks, Wildlife and Plant Conservation, Thailand
[email protected]
Contents of the presentation
1. Introduction
APFORGEN strategies
Global Plan of action on FRG
APFPORGEN and FAO collaboration
2. Importance and Situation of Dalbergia spp. and Shorea spp. in ASEAN
3. Working group on Conservation and sustainable use of Dalbergia spp.
and Shorea spp.
4. What have been initiated?
5. What need to be done and how to achieve the target?
Objectives of APFORGEN Strategy 2014-2016
1. Political and Financial support mobilized for implementation of
Global Plan of Action on Forest Genetic Resources in the Asia-Pacific
Region
2. Conservation and sustainable use strategies developed for
regionally important and threatened tree species
3. Tree seed programmes strengthened to facilitate ecosystem
restoration, support local livelihoods and climate change
adaptation and mitigation
APFORGEN and FAO Collaboration
>The mentioned objectives are aligned with and designed to support the
implementation of the Global Plan of Action on Forest Genetic Resources in
Asia-Pacific Region
>Global Plan of action is a strategic framework for the conservation and
sustainable use of genetic resources of trees and other woody plants
> It is based on State of the World’s Forest Genetic Resources report (FAO, 2014)
and adopted by Conference of FAO in 2013
>APFORGEN collaborated with FAO to coordinate the implementation of the
Global Plan of Action on FGR in the ASIA-Pacific region
The work under the objectives contributes in
particular, to the following Strategic Priorities of
global Plan of Action
1. Establish and strenghthen national FGR assessment,
characterization and monitoring system( Strategic Priority 1)
2. Promote the establishment and development of efficient and
sustainable ex situ conservation systems, including in vivo
collection and gene banks (Strategic Priority 6)
The work under the objectives contributes in
particular, to the following Strategic Priorities of
global Plan of Action
3. Develop and implement regional in situ conservation strategies and
promote ecoregional networking and collaboration
(Strategic Priority
4. Reinforce regional and international cooperation to support
education , knowledge dissemination, research, and conservation
and sustainable management of FGR (Strategic Priority 24)
APFORGEN working groups
1. Mobilizing Political and Financial Support for the Implementation of
Global Plan of Action for Forest Genetic Resources in the Asia Pacific
Region
2. Conservation and Sustainable Use Strategies for Regionally Important
and Endangered Tree Species
3. Strengthening Tree Seed Programmes to Facilitate Ecosystem
Restoration, Support Local Livelihoods and Climate Change Adaptatation
and Mitigation
Threatened Tree species
>IUNCN Red List of Threatened species : illegal logging for high
demand on trade and habitat conversion
• Dalbergia spp. : Dalbergia cochinchinensis (CETES appendix II) and
related species
• Shorea spp. : Shorea stenoptera
Time for ASEAN to act to end illegal, violent rosewood trade
Rosewood logs stored in a warehouse, Dong Ha, Vietnam (c) EIA
Non timber forest product: resin and oil
production from seeds
• Shorea stenoptera
• Shorea macrophylla
• Shorea robusta
Working group on Conservation and sustainable
use of Dalbergia spp. and Shorea spp.
This Objective has the following targets:
1. Genetic diversity of phylogeography of Dalberigia spp. and Shorea
spp. is assessed.
2. Germplasm of Dalbergia cochinchinnensis and related species is
collected from each country within their distribution range to
support their distribution range to support their conservation in situ
an ex situ, tree breeding and sustainable use in the region
3. Regional species and provenance trails are established for Dalbergia
spp. and Shorea spp. for gene conservation and breeding purposes
4. Regional networking and partnerships related to conservation,
management and sustainable use ( including but not restricted
Dalbergia and Shores spp.
Some genetic research has been initiated
• Genetic diversity of Dalbergia cochinchinensis in Thailand using SSR and
Chloroplast SNP (Changtragoon et al, 2017 )
• Identification of Dalbergia species and related species using SNP
(Changtragoon, 2015)
• Identification of illegal logged Dalbergia cochinchinesis individual trees
(Changtragoon, 2017)
• Genetic diversity of Dalbergia cochinchinesis in Asean using SSR
(Hartvig, et al. 2017)
Variable nucleotide sites which is specific to Dalbergia
cochinchinensis, Dalbergia oliveri, Dalbergia assamica,
Dalbergia cultrate and Pterocarpus indicus at Mat K
(713bp)
cpDNA locus
maturaseK (matK) 713 bp
1 1 111112
2223 333 444444444444555555 6666666 7
44 55880141778881226770334430112222335890014555133457870
409517257495690146445045131358890189134623727186147853209
0
7 7
36
0
8
8
3
Siamese rosewood
(Dalbergia
AACTTTACTGGTTTTGACGTACGGGGTCCTCATTCTCCCGCTCATGGGGCCTGGAGT
cochinchinensis )
Black wood
(Dalbergia oliveri)
AACTGTACAGGTTTCAAAGTACGGGGTACT - - - CATCTCACTCATTGAATATGGAAT
Ket dam
(Dalbergia
assamica )
AACTGTACAGGTTTCGACGTATGGGGTACT - - - CATCTCGCTCATTGAACATGGAAT
Kra phi khao khwai
(Dalbergia cultrata ) AACTGTACAGGTTTCGACGCACGGGGTACT
Burmese ebony
(Pterocarpus
indicus )
- - - CATCTCGCCCATTGAACATGTAAT
CCGGGCTTATACAGCGCCTTCCAATCCATA - - - CACATAGATAGGTAAACACAGGAC
Phylogenetic tree of Dalbergia cochichinensis and the
other Dalbergia spp.
Siamese rosewood
NLG11 Dalbergia
cochinchinensis
(Dalbergia
cochinchinensis
)
Kra phi khao khwai
(Dalbergia cultrata)
KPK Dalbergia cultrata
Dalbergia
spp.
Ket dam
(Dalbergia assamica)
NLG20 Dalbergia assamica
Black wood
chch
Dalbergia oliveri
(Dalbergia
oliveri )
Burmese ebony
(Pterocarpus indicus )
NLG18 Pterocarpus macrocarpus
Pterocarpus spp.
บริเวณหรือตำแหน่งในคลอโรพ
ึ ษำ
ลำสต์จโี นมทีใ่ ชศ้ ก
Methodology
VK/K
P
BT/
T
http://th.wikipedia.org/wiki
PK/N PL/
P
SK
PIK/
CC/
CP
MK
ND/
MK
TB/
MN/
KK/SB
BR
SB
PPT/
MD
D. cochinchinensis were sampled from 14 populations from
PT/U
PJ/U
Thailand
BDT/U
B
B
Location
Region
Populationnames Samplesize
Muakleknai, Saraburi
Kaengkhoi,Saraburi
Tapraya, Buriram
Dongnatam, UbonRatchathani
Phataem, UbonRatchathani
Phuchong-nayoi, UbonRatchathani
PhuPhaThoep, Mukdahan
PhuPha Lek, SakonNakhon
PaKhokKhaw, MahaSarakam
Na Dun, MahaSarakam
PhuLaenkha, Chaiyaphum
SilvicultureResearchCentre, KamphaengPhet
Phukao-Phuphankham,Nongbualamphu
Banhuton,Trang
total
Central
Central
Lower NorthEastern
Lower NorthEastern
Lower NorthEastern
Lower NorthEastern
Upper NorthEastern
Upper NorthEastern
Upper NorthEastern
Upper NorthEastern
Lower NorthEastern
Central
NorthEastern
South
MN/SB
KK/SB
TP/BR
DT/UB
PT/UB
PJ/UB
PPT/MD
PL/SK
PKK/MK
ND/MK
PlK/CP
VK/KP
PK/NP
BT/T
10
10
11
12
8
7
11
11
11
12
10
10
10
10
143
ประชำกรทีใ่ ช ้
ึ ษำ Location
ศก
Muakleknai, Saraburi
Kaengkhoi,Saraburi
Tapraya, Buriram
Dongnatam, UbonRatchathani
Phataem, UbonRatchathani
Phuchong-nayoi, UbonRatchathani
PhuPhaThoep, Mukdahan
PhuPha Lek, SakonNakhon
PaKhokKhaw, MahaSarakam
Na Dun, MahaSarakam
PhuLaenkha, Chaiyaphum
SilvicultureResearchCentre, KamphaengPhet
Phukao-Phuphankham,Nongbualamphu
Banhuton,Trang
total
Region
Populationnames
Samplesize
Central
Central
Lower NorthEastern
Lower NorthEastern
Lower NorthEastern
Lower NorthEastern
Upper NorthEastern
Upper NorthEastern
Upper NorthEastern
Upper NorthEastern
Lower NorthEastern
Central
NorthEastern
South
MN/SB
KK/SB
TP/BR
DT/UB
PT/UB
PJ/UB
PPT/MD
PL/SK
PKK/MK
ND/MK
PlK/CP
VK/KP
PK/NP
BT/T
10
10
11
12
8
7
11
11
11
12
10
10
10
10
143
PK/NP Nong Bua
Lamphu
PL/SK Sakon
Nakhon
PPT/MD
Mukdahan
VK/KP KamphaengPhet
CC/MK Maha
Sarakam
ND/MK Maha
Sarakam
KK/SB
Saraburi
PT/UB Ubon
Ratchathani
MN/SB
Saraburi
PJ/UB Ubon Ratchathani
BT/T
Trang
PlK/CP
Chaiyaphum
TP/BR
Buriram
DT/UB Ubon
Ratchathani
H1 H H1 H9 H8 H7 H6 H5 H4 H
2 11 0
3
H H
2 1
Polymorphic sites and cpDNA haplotypes based on sequences of the trnS-trnG,
trnV-trnM and trnC-petN1R in Dalbergia cochinchinensis
70
2
M
N/
SB
H1
T
T
T
G
6 bp
5 bp
A
T
T
C
T
-
H2
G
T
T
G
6 bp
5 bp
A
T
T
C
T
H3
H4
H5
T
T
T
C
C
T
T
T
T
G
A
G
T
T
T
T
T
T
C
C
C
T
T
T
G
5 bp
5 bp
AGT
AT
5 bp
A
A
A
H6
A
T
G
H7
T
T
T
G
5 bp
A
T
H8
T
T
T
G
6 bp
6 bp
ACAA
TC
ACAA
TC
ACAA
TC
6 bp
5 bp
A
H9
T
T
T
G
6 bp
5 bp
H1
0
H1
1
H1
2
tota
l
T
T
T
G
6 bp
T
T
T
G
T
T
C
G
K
K/
S
B
TP/
BR
DT/
UB
6
-
-
T
T
T
2
8
C
T
T
C
G
T
A
T
5 bp
C
6 bp
5 bp
6 bp
5 bp
PJ
/U
B
PL/
SK
PK
K/
MK
ND
/M
K
PlK
/CP
5
P
T/
U
B
5
7
11
8
5
9
V
K/
K
P
6
-
-
-
-
-
1
-
-
2
8
-
-
-
-
-
-
1
-
-
-
4
-
-
-
-
-
T
-
-
1
-
-
-
-
C
T
-
-
-
-
-
T
C
G
-
-
5
1
T
T
C
T
-
-
-
-
A
T
T
T
T
-
-
-
A
T
T
T
T
-
-
10
10
Nongbuala
phu
7
0
1
Mukdahan
3
9
0
Trang
20
6
Kamphaeng
Phet
6
2
1
256
Chaiyaphu
128
412
Nakhon
Maha
Sarakam
Maha
Sarakam
3
1
6
Ubon
Ratchathani
Sakon
3
8
7
Ubon
Ratchathani
Ubon
Ratchathani
1
3
6
Buriram
trnC-petN1R
S S
S S S
N N N N N
P P
P P P
Saraburi
trnV-trnM
SNP
SNP
haplotype
trnS-trnG
S S S SNP
N N N
P P P
Saraburi
Populations
8
PP
T/
M
D
6
-
-
5
-
6
-
-
1
-
-
1
-
4
3
16
5
-
-
-
-
-
9
-
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
9
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
-
-
12
-
-
2
-
-
-
-
-
-
-
-
-
2
11
10
8
7
11
11
12
12
10
10
11
10
14
3
B
T/
T
PK
/N
P
tot
al
9
64
Identification of illegal logged Dalbergia cochinchinensis
trees using SSR markers
ลักษณะทำงพันธุกรรม
ทีเ่ หมือนกัน
What need to be done and how to achieve the target?
Working group on Conservation and sustainable
use of Dalbergia spp. and Shorea spp.
This Objective has the following targets:
1. Genetic diversity of phylogeography of Dalberigia spp. and Shorea
spp. is assessed.
2. Germplasm of Dalbergia cochinchinnensis and related species is collected
from each country within their distribution range to support their
distribution range to support their conservation in situ an ex situ, tree
breeding and sustainable use
3. Regional species and provenance trails are established for Dalbergia
spp. and Shorea spp. for gene conservation and breeding purposes
4. Regional networking and partnerships related to conservation,
management and sustainable use ( including but not restricted
Dalbergia and Shores spp. in the region
Thank you for
your attention