Human Genetics
Nucleic Acid Purification
Innovations in Nucleic Acid Isolation
Saliva, Blood, Mouthwash
E.Z.N.A.® Blood DNA Kit
The system is available in a midi format for
processing up to 2 mL whole blood and maxi
format for processing 10 mL. Also available
is the E-Z 96 Blood DNA Kit that combines
HiBind® technology with 96-well plates.
Up to 200 µL whole blood can be purified
from each sample. The streamlined E-Z
96 procedure and high throughput format
allows for the processing of 96 whole blood
samples in under 1 hour.
Sample
A 260/280
A 260/230
Yield (μg)
1
1.86
1.97
75.2
2
1.85
1.84
93.9
3
1.84
1.94
77.4
4
1.85
2.08
158.0
5
1.86
2.06
182.0
6
1.82
1.78
127.9
7
1.95
1.94
107.0
8
1.83
1.89
115.2
9
1.85
2.02
60.7
10
1.86
1.91
82.4
Genomic DNA isolated using the E.Z.N.A.® Blood DNA
Midi Kit. DNA was purified from 4 mL saliva stored in
Oragene® tubes using the E.Z.N.A.® Blood DNA Midi
Kit. DNA was eluted in 500 μL and quantitated using the
Thermo NanoDrop® 2000 spectrometer.
200
200
150
150
Yield (μg)
The E.Z.N.A.® Blood DNA Kit provides rapid
total DNA isolation from 1 - 250 µL of fresh
and frozen anticoagulated whole blood. The
E.Z.N.A.® Blood DNA Kit can also be used
for the preparation of genomic DNA from
buffy coat, serum, plasma, bone marrow,
lymphocytes, platelets, and body fluids.
This kit allows for simultaneous processing
of multiple samples in less than 30 minutes.
DNA purified with the E.Z.N.A.® Blood
DNA method is ready for applications such
as PCR, Southern blotting, or restriction
enzyme digestion.
100
100
50
50
00
1
2
3 4
5
6 7
8
9 10 11 12 13 14 15 16
Samples
Yield of genomic DNA purified using the E.Z.N.A.®
Blood Midi DNA Kit. DNA was purified from packed
blood cells of 1 mL whole blood using the E.Z.N.A.®
Blood DNA Midi Kit. DNA was eluted in 500 μL and
quantitated using the Thermo NanoDrop® 2000.
Product
Preparations
E.Z.N.A.® Blood DNA Kit
D3392-00
5
D3392-01
50
D3392-02
200
E.Z.N.A.® Blood DNA Midi Kit
D3494-00
2
D3494-01
10
D3494-02
50
D3494-03
100
Product
Preparations
E.Z.N.A.® Blood DNA Maxi Kit
D2492-01
10
D2492-02
20
D2492-03
50
E-Z 96 Blood DNA Kit
D1192-00
1 x 96
D1192-01
4 x 96
D1192-02
20 x 96
Saliva, Blood, Mouthwash
Mag-Bind® Blood DNA Kit
The Mag-Bind® Blood DNA Kit allows
rapid and reliable isolation of high-quality
genomic DNA from 10 - 200 µL blood
samples. It can be used in conjunction
with automated purification protocols on
robotic workstations using 96-well or 24well formats. The system combines the
reversible nucleic acid binding properties
of Mag-Bind® magnetic particles with the
time-proven efficiency of Omega Bio-tek’s
blood DNA isolation system to provide a
fast and convenient blood DNA isolation
method. The magnetic particle technology
isolates high-quality DNA that is suitable
for direct use in most downstream
applications such as qPCR, PCR, Southerns,
microarrays, and Next-Gen sequencing.
# Correct
# Errors
Reproducibility
1
727975
73
0.999950
2
727782
69
0.999953
3
728892
40
0.999973
4
728908
57
0.999961
5
728293
61
0.999958
6
728518
53
0.999964
7
729178
50
0.999966
8
729332
42
0.999971
9
729385
28
0.999981
10
729412
30
0.999979
11
729078
79
0.999946
12
727494
123
0.999916
Illumina Infinium Whole Genome Genotyping
Chip (OmniExpress): Average Call Rate >99%
DNA was isolated from 200 μL of blood using the
Mag-Bind® Blood DNA Kit 96 Kit. Two hundred ng of
DNA was processed and hybridized to the Illumina®
HumanOmniExpress BeadChips, following the standard
Illumina® protocol. In all, over 730,000 markers were
genotyped for each sample. Call rates and reproducibility
were calculated using the Illumina® BeadStudio
software.
Product
Preparations
Mag-Bind® Blood DNA Kit
M6221-00
5
M6221-01
50
M6221-02
200
Mag-Bind® Blood DNA Mini 96 Kit
M6211-00
1 x 96
M6211-01
4 x 96
M6211-02
20 x 96
Mag-Bind® Blood DNA 96 KF Kit
M6321-00
1 x 96
M6321-01
4 x 96
M6321-02
20 x96
PCR Detection Sensitivity
M
Blood
Plasma
Genomic DNA purified using the MagBind® Blood DNA Kit. Two ng plasmid DNA
was added into 200 µL of whole blood, then
isolated using the Mag-Bind® Blood DNA 96
KF with 200 µL as the elution volume. 2 µL
DNA was used as template in PCR reaction.
Saliva, Blood, Mouthwash
SQ Blood DNA Kit
Product
Preparations
D5032-00
10 mL
D5032-01
50 mL
D5032-02
150 mL
D5032-03
300 mL
8
7
Yield (µg)
The SQ Blood DNA Kit is a solution-based
system based upon the salt-out method
which isolates genomic and mitochondrial
DNA from variable amounts of fresh and
frozen whole blood. The SQ Blood DNA Kit
first lyses the red blood cells and pellets white
blood cells (WBC). The WBC are then lysed
and proteins are removed from solution. The
cleared lysate is then transferred and DNA is
precipitated by isopropanol. In addition buffy
coat, bone marrow, or cultured cell samples
can also be used. This flexible procedure can
be scaled up from a starting sample volume
of 0.1 mL to 20 mL and completed in less than
90 minutes. DNA purified using the SQ Blood
DNA Kit is free of contaminants and enzyme
inhibitors making it suitable for downstream
applications such as PCR, restriction digests
and Southern blotting.
6
5
4
3
2
1
0
1
2
3
4
5
6
7
Expected Yield from 200 µL whole blood. Genomic
DNA was isolated from 7 200 µL whole blood
samples stored in EDTA using the SQ Blood DNA
Kit. O.D. ratio and yield was calculated using a
Thermo Nanodrop® 2000c.
SQ II Blood DNA Kit
The SQ ll Blood DNA Kit offers a fast and
affordable way to isolate high molecular
weight DNA from varying amounts of whole
blood samples. Buffy coat or cultured cells
can also be used with this kit. The quick single
tube procedure first lyses the cell membrane.
Then the nuclei are lysed and genomic DNA
precipitated out of solution with isopropanol
yielding high-molecular weight DNA. Single
or multiple samples can be processed in less
than 60 minutes, and can be easily scaled up
and down according to the amount of starting
material.
Product
Preparations
D0714 - 50
50 mL
D0714 - 250
250 mL
Saliva, Blood, Mouthwash
E.Z.N.A.® PX Blood RNA Kit
12.5
Fluorescence
E.Z.N.A.® PX Blood RNA Kit is designed for
isolation of total RNA from blood samples
stored in special preservation reagents and
Paxgene® tubes. The procedure completely
removes contaminants and enzyme
inhibitors making RNA isolation fast,
convenient, and reliable. The RNA purified
using the E.Z.N.A.® PX RNA Kit method
is ready for applications such as RT-PCR.
28S
18S
10.0
7.5
5.0
2.5
0.0
19 24 29 34
39
44 49 54 59 64 69
Time (seconds)
Product
Preparations
E.Z.N.A.® PX Blood RNA Kit
R1057-00
5
R1057-01
50
R1057-02
200
RNA extracted from whole blood stored
in Paxgene® Blood RNA tubes by using the
E.Z.N.A.® PX Blood RNA Kit was analyzed on
an Agilent Bioanalyzer 2100.
E-Z 96 PX Blood RNA Kit
R1058-00
1 x 96
R1058-01
4 x 96
R1058-02
12 x 96
Mag-Bind® PX Blood RNA 96 Kit
Product
Preparations
M7763-00
1 x 96
M7763-01
4 x 96
M7763-02
12 x 96
The Mag-Bind® PX Blood RNA Kit allows
for the isolation of total RNA from 2.5
mL of whole blood that has been stored
in Paxgene® Blood RNA tubes. Stabilized
blood samples are first spun down and
the nucleic acid pellets are washed
and recollected. After washing nucleic
acid pellet is dissolved into a specially
formulated resuspension buffer. At this
point the samples can be transferred to
96-well plates for processing in robotic
liquid handlers and magnetic processors.
High-quality intact RNA can be isolated
isolated in less than 90 minutes.
Buccal Swabs
E.Z.N.A.® Tissue DNA Kit
The E.Z.N.A.® Tissue DNA Kit offers a
simple, rapid, and cost effective method
for the isolation of DNA from a wide
variety of sample sources including buccal
swabs. After cell lysis, the DNA purification
process can be completed in less than
30 minutes. Buccal swab cells are first
detached with PBS solution. Along with
protease, our enhanced lysis buffer then
quickly and efficiently lyses the cells to
allow the release of DNA. Ethanol is then
added to enhance DNA binding conditions
to the HiBind® DNA column. Once bound,
the DNA is subsequently washed with an
ethanol-based solution and eluted from
the column. Single or multiple samples
can be simultaneously processed with
this spin-column based kit. DNA purified
using the E.Z.N.A.® Tissue DNA Kit is ready
for most downstream applications such
as PCR, Southern blot, and restriction
enzyme digestion.
Product
Preparations
E.Z.N.A.® Tissue DNA Kit
D3396-00
5
D3396-01
50
D3396-02
200
E-Z 96 Tissue DNA Kit
D1196-00
1 x 96
D1196-01
4 x 96
D1196-02
20 x 96
Rat kidney
M
T
A
P
Omega
Q
M
T
A
P
Omega
Q
M
Genomic DNA purifed using the E.Z.N.A.® Tissue DNA Kit.
Genomic DNA was purified from 10 mg fresh rat kidney,
was isolated with the E.Z.N.A.® Tissue DNA Kit, Company
T, Company A, Company P, and Company Q’s Tissue DNA
Kit. Genomic DNA (3% of total purified DNA) was analyzed
on a 0.8% agarose gel to demonstrate yield and quality of
the DNA.
M: Lambda-HindIII
Sperm 100 μL
OD 260/280
Yield
1
2
3
1.86
1.84
1.88
13.2 μg
10.5 μg
8.9 μg
Yield of sperm samples using the E.Z.N.A.® Tissue DNA Kit.
Genomic DNA was purified from 100 μL sperm by modified
E.Z.N.A.® Tissue DNA Kit. 100 μL Elution Buffer was used to
elute DNA. The OD reading is done by Beckman Coulter DU® 640
spectrophotometer.
Buccal Swabs
Mag-Bind® Tissue DNA Kit
Yield (μg)
The Mag-Bind® Tissue DNA Kit allows
rapid and reliable isolation of high-quality
genomic DNA from buccal swabs. This kit
allows single or multiple simultaneous
processing various swab types such
as Whatman Omni Swab, Epicentre
MasterAmp™ Buccal brush swab, or soft
foam tip swabs in under 1 hour. Buccal
swabs are pretreated with Proteinase K
and lysed in a specially formulated buffer
containing detergent. DNA then binds
to the surface of Mag-Bind® magnetic
particles. Proteins, polysaccharides, and
cellular debris are efficiently removed
with two wash steps. Pure DNA is eluted
in water or low ionic strength buffer.
DNA purified using the Mag-Bind® Tissue
DNA Kit is ready for applications such as
PCR, Southern blotting, and restriction
enzyme digestion. This kit can be used
in conjunction with automated work
stations.
1
2
3
4
5
6
7
8
Sample ID
Average Yield of buccal swab samples using the Mag-Bind® Tissue
DNA Kit. Buccal swab samples were processed by the Mag-Bind®
Tissue DNA 96 Kit using the buccal swab protocol. Samples were
collected from 8 people in triplicate. DNA was eluted with 100 µL
Elution Buffer. Yield was calculated using Thermo NanoDrop® 2000.
Product
Preparations
Mag-Bind® Tissue DNA Kit
M6223-00
5
M6223-01
50
M6223-02
200
Mag-Bind® Tissue DNA 96 Kit
M6229-00
1 x 96
M6229-01
4 x 96
M6229-02
20 x 96
Mag-Bind® Tissue DNA 96 KF Kit
M6329-00
1 x 96
M6329-01
4 x 96
M6329-02
20 x 96
Bacterial
E.Z.N.A.® Stool DNA Kit
The E.Z.N.A.® Stool DNA Kit allows rapid
and reliable isolation of high-quality
total DNA from fresh and frozen stool
samples. Up to 200 mg stool samples can
be processed in less than 60 minutes.
Efficient solution-based inhibitor removal
replaces the need for special tablets or
precipitates. The system combines the
reversible nucleic acid-binding properties
of the HiBind® matrix with the speed and
versatility of spin column technology to
eliminate humic acid, polysaccharides,
phenolic compounds, and enzyme
inhibitors from stool samples. Purified
DNA is suitable for PCR, restriction
digestion, and hybridization techniques.
Product
Preparations
D4015-00
5
D4015-01
50
D4015-02
200
Before Inhibitor Removal Treatment
After Inhibitor Removal Treatment
Image of sample comparing the effectiveness of HTR Reagent.
The image shows comparison of DNA crude extract before and
after applying the inhibitor removal solution to each sample.
Mag-Bind® Stool DNA Kit
By combining Omega Bio-tek’s novel and
proprietary Stool DNA method with the
high-performance Mag-Bind® technology,
the Mag-Bind® Stool DNA Kit provides
a fast and easy method for isolation
of genomic DNA from stool samples
containing high humic acid contents. The
system allows detection of pathogens and
human DNA and also provides efficient
DNA purification from gram-negative
and gram-positive bacteria. The purified
DNA is ready to use in most downstream
applications such as PCR, Southern blot
and SNP analysis.
Product
Preparations
M4015-00
5
M4015-01
50
M4015-02
200
Bacterial
E.Z.N.A.® Bacterial DNA Kit
The E.Z.N.A.® Bacterial DNA Kit allows the
rapid and reliable isolation of high-quality
genomic and plasmid DNA from grampositive and gram-negative bacteria. This
kit uses optimized lysis conditions to
isolate DNA from bacterial cells or bodily
fluids.
The cell wall is removed by lysozyme
digestion and bead beating, followed by
protease digestion. After lysis, binding
conditions are adjusted and the sample
is applied to a HiBind® DNA spin-column.
Three rapid wash steps remove trace salts
and protein contaminants, and DNA is
eluted in water or Elution Buffer. Purified
DNA can be directly used in downstream
applications without the need for further
purification.
M
1
2
3
Genomic DNA purified using the E.Z.N.A.®
Bacterial DNA Kit. Purified genomic DNA,
from various bacteria, was isolated with the
E.Z.N.A.® Bacterial DNA Kit. DNA was extracted
in duplicate from each of the following samples:
sample 1 from S. aureus, sample 2 from B.
subtilis, and sample 3 from P. aeruginosa.
Genomic DNA (5% of total purified DNA) was
analyzed on a 1% agarose gel to demonstrate
yield and quality of the DNA.
Product
Preparations
D3350-00
5
D3350-01
50
D3350-02
200
Mag-Bind® Bacterial DNA 96 Kit
Product
Preparations
M2350-00
1 x 96
M2350-01
4 x 96
M2350-02
20 x 96
The Mag-Bind® Bacterial DNA 96 Kit allows
rapid and reliable isolation of high-quality
genomic DNA from a wide variety of grampositive or gram-negative bacteria from
nasal or lung aspirates, urine and other
body fluids . 200 µL of body fluids can be
directly used for DNA extraction while
larger sample volumes bacterial and host
cells must be pelleted prior to processing.
The key to the system is Omega Bio-tek’s
proprietary magnetic particles that avidly,
but reversibly bind DNA under optimized
conditions allowing proteins and other
contaminants to be removed.
Viral
E.Z.N.A.® Viral RNA Kit
Product
Preparations
R6874-00
5
R6874-01
50
R6874-02
200
Amplification
3000
RFU
The E.Z.N.A.® Viral RNA Kit is designed for
the isolation of viral RNA from acellular
fluids such as plasma, serum, and urine.
The kit can also be used to isolate viral
DNA, genomic DNA, circulating DNA and
total RNA. The procedure completely
removes contaminants and enzyme
inhibitors making viral RNA isolation fast,
convenient and reliable. RNA purified
using the E.Z.N.A.® Viral RNA method is
ready for all downstream applications
such as RT-PCR and qPCR. This kit has
been validated for isolating viral nucleic
acids from hepatitis A, B, C, and HIV.
2000
1000
0
0
10
20
30
Ct values of recovered RNA isolated using the E.Z.N.A.® Viral
RNA Kit. Hepatitis B virus was isolated using the E.Z.N.A.® Viral
RNA Kit. A 10-fold dilution series of the recovered RNA was used
in a SYBR® Green-based real-time PCR reaction. Each reaction
was performed in triplicate.
E-Z 96 Viral RNA Kit
Product
Preparations
R1074-00
1 x 96
R1074-01
4 x 96
R1074-02
12 x 96
40
Cycles
Based on the same principle of E.Z.N.A.®
Viral RNA Kit, the E-Z 96 Viral RNA kit is
designed for isolation of viral RNA samples
from acellular samples such as plasma,
serum, urine, and cell culture supernatant
in a 96-well plate format. The procedure
completely removes contaminants and
enzyme inhibitors making viral RNA
isolation fast, convenient, and reliable.
This kit has been validated for isolation
viral nucleic acids from hepatitis A, C, and
HIV. The kit is also suitable for isolation of
total RNA from cultured cells, tissues, and
bacteria. RNA purified using the E-Z 96
Viral RNA method is ready for applications
such as RT-PCR.
Viral
Mag-Bind® Viral DNA/RNA Kit
The Mag-Bind® Viral DNA/RNA Kit isolates
DNA and RNA from serum, plasma, swabs,
cell culture supernatants, urine and other
cell-free bodily fluids. This kit uses a unique
system to efficiently lyse viral samples.
The high binding ability and sensitivity of
the magnetic particle technology allows
efficient isolation from low titers or large
sample sizes.
Product
Mag-Bind® Viral DNA/RNA Kit
1 ×10 viral particles/ µL
23.09
22.99
25.48
1 ×10 viral particles/µL
5
25.53
25.08
28.64
1×104 viral particles/µL
28.56
28.66
31.23
1×10 viral particles/µL
3
50
M6245-02
200
M6246-01
1 x 96
M6246-02
4 x 96
M6246-03
12 x 96
3000
19.88
2000
31.59
31.58
RFU
23.09
1 ×10 viral particles/µL
M6245-01
Amplification
19.98
6
5
Ct value
19.90
7
M6245-00
Mag-Bind® Viral DNA/RNA 96 Kit
This system can be used on multiple
automated platforms including Ambion®
MagMAX™, Thermo Kingfisher® Flex,
Beckman Coulter Biomek FX and Hamilton
Star instruments.
RNA template
Preparations
1000
0
0
10
20
30
40
Cycles
Ct values of recovered RNA isolated using the MagBind® Viral RNA/DNA Kit. Serum was separated from
a human blood sample containing 1 x 107 Hepatitis B
viral particles/μL, A 10-fold dilution series of the serum
was performed and 50 μL of each dilution was used
in the Mag-Bind® Viral RNA/DNA Kit to isolate viral
RNA. 2 μL of RNA was used as the template. A 10-fold
dilution series of the recovered RNA was used in a SYBR
Green-based real-time PCR reaction. Each reaction was
performed in triplicate.
FFPE
E.Z.N.A.® FFPE DNA Kit
The E.Z.N.A.® FFPE DNA Kit is designed
for fast and easy purification of DNA from
formalin-fixed paraffin-embedded (FFPE)
tissue sections. Paraffin removal can be
performed using a xylene and ethanol
method or an efficient heat treatment.
Samples are incubated in a specialized lysis
buffer along with Proteinase K to reverse
crosslinking effectively releasing short
and long DNA fragments. After adjusting
the binding conditions with ethanol, the
lysate is applied to the MicroElute® DNA
Column to bind DNA. Cellular debris and
proteins are effectively removed during
the wash steps. High-quality purified DNA
is then eluted in Elution Buffer or water
and is ready for applications such as PCR
and Next-Gen sequencing.
Product
Preparations
D3399-00
5
D3399-01
50
D3399-02
200
M A1 A2 A3 O1 O2 O3
Sample
260/280
260/230
Yield, µg
O1
1.79
2.25
13.25
O2
O3
1.79
1.80
2.18
2.30
13.32
12.48
A1
A2
A3
1.80
1.81
1.82
2.31
2.24
2.27
9.53
9.28
6.71
Quality and yield of DNA with agarose gel analysis
comparing Company A’s FFPE Kit and Omega Bio-tek’s
E.Z.N.A.® FFPE DNA Kit. Analysis was completed on
a 0.5% agarose gel. Lanes 1-3 on represent Company A
and Lanes 4-6 Omega Bio-tek’s E.Z.N.A.® FFPE DNA Kit,
M: 1 kb Marker. FFPE DNA tissue starting material was
~2 mg post-deparaffinization. An overnight digestion was
completed at 55°C. Samples were eluted in 60 µL Elution
Buffer. O.D. ratio and yield was calculated using a Thermo
Nanodrop® 2000c.
FFPE
E.Z.N.A.® FFPE RNA Kit
The E.Z.N.A.® FFPE RNA Kit is specially
designed for isolation of total RNA from
formalin-fixed, paraffin-embedded tissue
sections or punches. This method can also
be used for the preparation of total RNA
from fibrous samples such as heart and
muscle.
Paraffin can be removed by either a xylene
treatment or a special heating procedure
which also deactivates RNases. The
sample is lysed with specialized lysis buffer
and Proteinase K to reverse crosslinking.
Genomic DNA is then removed from
the lysate by way of a DNA Clearance
Column. Once binding conditions have
been adjusted with ethanol, RNA from
the filtrate is subsequently bound to the
MicroElute® RNA Column. Proteins and
cell debris are effectively removed during
wash steps and RNA is eluted in DEPC
water. The E.Z.N.A.® FFPE RNA Kit allows
purfication of high-quality RNA though not
recommended for to use in downstream
applications requiring full-length RNA due
to the fragmentation caused during the
fixation process.
FFPE RNA Kit Protocol
Remove paraffin and
reverse cross linking
Adjust the binding
conditions
Bind genomic DNA with
DNA Clearance Column
Bind RNA
Wash column
Dry column
Product
Preparations
R6954-00
5
R6954-01
50
R6954-02
200
Elute RNA
FFPE
Mag-Bind® FFPE RNA Kit
Amplification
105
104
RFU
The Mag-Bind® FFPE RNA Kit is specially
designed for isolation of total RNA from
formalin-fixed, paraffin-embedded tissue
sections. The sample is first heated in
a special buffer to deactivate RNase/
DNase and deparaffinize the sample.
Then the lysate is treated with Proteinase
K to digest proteins. The cleared lysate
is then mixed with Mag-Bind® magnetic
particles to capture the RNA and genomic
DNA is subsequently removed by DNase
I digestion. After three quick wash steps,
purified DNA is eluted with Elution Buffer
or nuclease-free water.
105
102
0
10
20
30
40
Cycles
Standard Curve
30
28
(Ct)
26
Product
Preparations
Mag-Bind® FFPE RNA Kit
24
22
20
18
M2555-00
5
M2555-01
50
0
M2555-02
200
SYBR: E=100.9% R�2 = 0.995 Slope = -3.300
16
1
2
3
4
Mag-Bind® FFPE RNA 96 Kit
M2551-00
1 x 96
M2551-01
4 x 96
M2551-02
20 x 96
Mag-Bind® FFPE RNA 96 KF Kit
M6953-00
1 x 96
M6953-01
4 x 96
M6953-02
20 x 96
qPCR analysis of RNA isolated from FFPE tissue using
the Mag-Bind® FFPE RNA Kit. A 10-fold dilution series
of the recovered DNA was used in a SYBR Green-based
real-time PCR targeting a 80-bp fragment of the human
ribosomal protein gene RPL13a. Each reaction was
performed in triplicates. The input DNA amounts were
50, 5, 0.5 and 0.05 ng, respectively. Standard curve for
10-times serial diluted DNA. Detection using SYBR Green.
FFPE
Mag-Bind® FFPE DNA Kit
The Mag-Bind® FFPE DNA kit provides a
rapid and easy method for the isolation
of total DNA from formalin-fixed, paraffinembedded (FFPE) tissue sections. Due
to fixation and embedding procedures,
nucleic acids in FFPE samples are
heavily fragmented and modified by
formaldehyde. While the Mag-Bind®
FFPE DNA kit is optimized to minimize the
effect of the formaldehyde modification,
it is not recommended to use the DNA
purified with this kit for downstream
applications that requires full length DNA.
The standard protocol uses a heating step
instead of xylene to remove paraffin from
FFPE sample as in the alternative protocol.
Mag-Bind® particles allow for automationfriendly extraction from FFPE tissue in
under 6 hours. Purified DNA is suitable
for downstream applications including
SNP analysis, Next-Gen sequencing, and
genotyping. This system is compatible
on Hamilton® STAR, Beckman Coulter
Biomek® FX, Biomek® 2000, Tecan
Freedom® Evo, Thermo Kingfisher® Flex
and other workstations.
Product
Preparations
Mag-Bind® FFPE DNA Kit
M6957-00
5
M6957-01
50
M6957-02
200
Mag-Bind® FFPE DNA Kit 96
M6958-00
1 x 96
M6958-01
4 x96
M6958-02
20 x 96
Mag-Bind® FFPE DNA 96 KF Kit
M6954-00 1 x 96
M6954-00 4 x 96
M6954-00 20 x 96
Omega Bio-tek Mag-Bind® FFPE DNA Kit
vs.
Company A FFPE purification protocol
32
31
Company A spin column
30
Omega Bio-tek Mag-Bind FFPE DNA Kit
29
0.0
0.2
0.4
0.6
0.
28
27
26
25
24
23
00 10
11
10
100
DNA was isolated from FFPE Samples using the MagBind® FFPE DNA Kit and Company A’s FFPE RNA and
DNA isolation following standard protocols. A serial
dilution of recovered DNA was then used in a (SYBR®)
real-time q-PCR. Each reaction was performed in triple
replicates. The points represent input DNA amounts of
50, 5, 0.5 and 0.05 ng (right to left) per reaction.
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