Dermatophyte PCR kit
The Dermatophyte PCR Kit is a multiplex PCR system that
enables determination of dermatophytes generally and T.
rubrum from nail samples within 5 hours.
General
Nail infections are caused chiefly by T. rubrum and T. mentagrophytes.
Traditionally, these species are identified from nail samples by culturing on
medium, which takes 10­15 days to 3­4 weeks. The Dermatophyte PCR Kit is
a multiplex PCR system that enables determination of dermatophytes
generally and T. rubrum from nail samples within 5 hours. The primer mix
contains two primer pairs aimed at, respectively, chitin synthase 1 for
detection of dermatophytes generally and ITS2 (internal transcribed spacer)
to detect T. rubrum. Furthermore, the primer mix contains an internal
plasmid control that functions as a template for the primer pair specifically
for T. rubrum.
By comparing the dermatophyte multiplex PCR method with conventional
methods of identifying nail infections, it has been shown that the PCR
method identifies most dermatophyte and T. rubrum­positive nail samples
(ref. 1, 2 and 3). Product description
The kit contains all reagents necessary to carry out dermatophyte PCR
reactions.
The kit contains Buffer A and Buffer B to prepare the template, primer mix
and PCR Ready Mix (including loading buffer) to run the samples and two
PCR­positive DNA controls—one containing dermatophyte genomic DNA and
one containing T. rubrum genomic DNA.
There is enough reagent for 100 PCR reactions in the kit. Use
The Dermatophyte PCR Kit is used for in vitro diagnostics of dermatophytes
in general and T. rubrum from nail samples. Product range
Article
number
Product
Number
of tests
Packing
73411 Dermatophyte
PCR­kit
100 1 box
References
1. A. Brillowska­Dabrowska, D. M. Saunte and Maiken Cavling Arendrup.
Five­hour diagnosis of dermatophyte nail infection with specific detection of
Trichophyton rubrum. Jour Clin Microbiol (2007) 1200­1204.
2. N. Kondori, A.­L. Abrahomsson, N. Ataollahy and C. Wennerås.
Comparison of a new commercial test, Dermatophyte­PCR kit, with
conventional methods for rapid detection and identification of Trichophyton
rubrum in nail specimens. Medical Mycology (2010), Early Online, 1­4.
3. A. Brillowska­Dabrowska, S. S. Nielsen, H. V. Nielsen and M. C. Arendrup.
Optimized 5­hour multiplex PCR test for the detection of tinea unguium:
performance in a routine PCR laboratory. Medical Mycology 2010, 48, 828­
831
4. Nisha Suyien Chandran, Jiun­Yit Pan,Zacharias AD Pramono, Hiok­Hee
Tan and
Chew­Swee. Complementary role of a polymerase chain reaction test in the
diagnosis of onychomycosis. Australasian Journal of Dermatology (2013) 54,
105–108
Downloads
Brochures
Dermatophyte PCR Kit
Packaging inserts
Dermatophyte PCR kit
Material safety data sheets
Dermatophyte PCR kit
Declarations of Conformity
DoC Dermatophyte PCR
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Dermatophyte PCR kit
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