OLYMPUS NEOPAK
INCIDENT LIGHT
MICROSCOPE
INSTRUCTION MANUAL
MOOELN
CONTENTS
I. STANDARD SET'
1
IT. PRINCIPAL PARTS .. ·
m.
·
2
SPECIFICATIONS
IV. OPTICAL PATH DIAGRAM
V. STRUCTURE .. ·.. ··
3
·
4
·......
VI. HOW TO ASSEMBLE
VIT. HOW TO USE· .. ········
2
7
··
·.. ·.. ·.. ·.. ·.. ·······..
A.
Adjustment of the movement of coarse adjustment handle
B.
Place the specimen on the stage
C.
Centering of the light source lamp
D.
Focusing
.. . .. . ... ... ... ... ... . .. . .. . .. . .. . .. ... ... ... . .. ... . .. ...
9
10
E. Interpupillary Distance and Diopter Adjustments
F.
Bright field and dark field observation
G.
Oblique illumination
H.
Observation with polarized light
I.
Photography
11
Vlll, CHARACTERISTICS OF LENSES .. ·.. ·.. ·................
12
IX. IMPORTANT POINTS TO REMEMBER·
12
·
I . STANDARD SET
N-Mono
Description
N·Bi
N·Tr
Main body of microscope (including the mirror)
1 set
1 set
1 set
Microscope head (including eyepiece tube cap)
Mono. 1
Bi. 1
Tri 1
Stage (with stage insert plate)
1
1
1
Illuminator
1
1
1
NeoSX, Neo10x, Neo40x
1 each
1 each
1 each
BiP7 x, BiWF10 x, BiWF1S x
--
1 pair each
1 pair each
--
1
--
1 each
--
1 each
Spare bulb 6V2A
2
2
2
Metal slide
S
5
S
Sector diaphragm (180°, 270°)
1 each
1 each
1 each
Mount for sector diaphragm
1
1
1
1 each
1 each
1 each
Transformer
1
1
1
Dust plug for objective mounting socket on nosepiece
1
1
1
Vinyl cover
1
1
1
Case
1
1
1
Certificate of inspection
1
1
1
Objectives:
Eyepiece:
Straight tube for Photomicrography
(including eyepiece tube cap)
Eyepiece for Photo: P7x, P10x, P1Sx
e2.S ¢ mm Y-48
Filters 32.59 mm C
32.S 9 mm G-S33
+Special accessory:
• Polarizing equipment (polarizer and analyser)
+Olympus objective has its colour band
. Tn,,: OLYMPUS OBJECTIVE, you just purchased, has a colour band on it.
This new step has been adopted for your convenience, that you may understand
the magnification, if you only see the colour, without looking at the magnification
number engraved on the tube.
Magnification
Neo Sx
Neo lOx
Neo 40x
Colour
Red
Orange
Brilliant
green
-1
II. PRINCIPAL PARTS
Eyepiece
Illuminator
Binocular head
Clamping screw
Revolvi ng nosepiece
Stage
Objective
Transformer
Focusing mechanism
Mirror
ill. SPECIFICATIONS
Microscope head:
Monocular and binocular; inclinded 45°
to the horizontal plane,
rotates 360°.
Trinocular:
The binocular head is inclined 45° to the horizontal plane,
rotates 360°.
Revolving Nosepiece:
Quadruple (ball
A straight tube for photomicrography.
bearing type)
with the marks for mounting
objectives
Mechanical tube length:
Total magnification:
Focusing:
200mm
35 X -600 X
Raising and lowering of the stage by the coarse and fine adjustment knobs.
Coarse adjustment:
Fine adjustment:
Rack and pinion system, Range of vertical movement: 47.5mm
By lever, range of vertical
movement:
minimum graduation:
Stage:
2 mm
0.005 mm
Square, with a cross· movement mechanical stage, coaxial stage h:mdle.
Transformer:
Illuminator:
Rated 6V5A, with a voltmeter.
Incident type illumination capable of bright field and dark field illuminations.
Oblique illumination is also possible.
Weight:
Aprox.
6kg (the main body only)
Height:
Aprox.
370mm (N-Bi)
2 -
IV. OPTICAL PATH DIAGRAM
A. Incident light illumination for bright field
The light from the light source is reflected by the half mirror, passes through the objective and
illuminates the specimen.
The rays of illumination reflected from the specimen enter the objective,
passes through the half mirror and the eyepieces and reaches the observer's eyes.
Halfmirror
Objective
B. Incident light illumination for dark field
The light from the light source passes through the ring slit and is reflected by the ring mirror and
illuminates the specimen after passing through the ring condenser located around the objective.
Since the rays of light reflected from the specimen enter the objective, you can see the details of
the specimen through eyepieces, which cannot be seen against the bright field.
Eyepiece
Prism
Ring mirror
Objective
Ring slit
lUng condenser-
-
3 -
V. STRUCTURE
A. Main Body
Illuminator mount
Arm
The illuminator is
mounted here.
Fine adjustment handle
By turning this handle,
the stage is moved finely
up and down within a
range of 2mm
Revolving Nosepiece
Dovetail mount for stage
The stage is installed
here
Hole for mounting
the mirror
Coarse adjustment handle
By turning this handle,
the stage is moved up
and down within a range
of 47.5mm
Base
o
Revolving Nosepiece
The 4-hole revolving nosepiece operates very smoothly with a rotating steel ball on the axis of
rotation and the knurled rim.
The built-in click and ratchet mechanism correctly positions the
objective set, maintaining proper optical alignment.
Markings
A,
B, C
and D are given on
the nosepiece to indicate the positions of the objectives 5 x. 10 X, 40 ~<. and the dust plug to be
mounted in that order so that the magnification of the lens in use may be known at a glance.
B. Microscope Head
The following four types of microscope heads are available:
* Monocular head
• Binocular head
* Trinocular head
• Straight head for photomicrography
The head can be mounted on to the arm by means of the knurled fixing ring, firmly and in precise
optical alignment.
The monocular and binocular heads and the binocular part of the trinocular
head are inclined 45 degrees from the horizontal plane.
The head can be revolved through 360 degrees, which permits observation from any desired direction.
Two different types of optical tubes are available for binocular head and trinocular head, so that
the user may choose the optical tube according to the physiology of his eyes.
(I)
Binocular head
:===~--Diopter
Adjustment Rings
Clamping SlTe\V----1. ..-::;IL-J'-.~._=
®
Trinocular Head
- - - - - - Photo Tube
Light Path Selector Lcver----.../
,:J
Straight head for photomicrography
When the monocular or binocular head is used, it must be replaced with the straight head for
ohotographic work.
The photomicrographic apparatus must be mounted on this straight head.
-
5 -
C. Illuminator
Lamphouse locking knob
The lamphouse can be
removed or fastened
by turning this knob.
Analyzer positioning pin
Microscope head mount
Socket locking knob
The socket can be
removed or fastened by
turning this knob.
Analyzer rotating knob
For rotating the analyzer
horizontally for observa.
tion with polarized light.
Socket
Optical path selector knob
The optical path can be
switched for bright field
illumination by pulling
out the knob, and for
dark field illumination
by pushing it in.
Secondary cord
Polarizer slot
Lamp centering knob
Lamphouse
Filter slot
D. Stage
Stage clip
Vernier
This scale reads to
O.lmm
I
Stage insert plate
Lateral movement scale
(50 -
120)
Back-forth movement scale
(0 - 50)
Back-forth movement handle
To move the stage 50
mm forward and backward by rack and
pinion mechanism.
Vernier
This scale
reads to
O.lmm
Stage locking lever
Lateral movement handle
To move the stage 70
mm side to side by the
lead screw.
Loosen this lever and
fit the stage into the
dovetail groove of the
microscope body and
lock it in place by
fastening the lever.
•
E. Transformer
Stopper
Voltage control knob
Turn it while pushing
it down.
When the \l mark is
at the left extreme,
the brightness has the
highest intensity.
Brightness of lamp can
be adjusted by turning
this knob.
o adjustment
screw
Turn this screw to make
correct 0 adjustment
Pilot lamp
Main switch
F. Mirror
Generally the metallurgical microscope is
used for observation of an opaque substance
specimen with reflecting light. except when
Mirror
Plane surface and
concave surface.
using the low power objective. in which case
Fork
the observation is performed with trans·
illumination.
Mirror mount
• VI. HOW TO ASSEMBLE
A. Install the stage
1.
Raise the focusing assembly fully by turning the
coarse adjustment handle.
2.
Turn up the stage locking lever
<D and fit the
guide on the stage into the guide ®
on
the
microscope body and slowly lower the stage until
it comes to a stop.
In installing the stage, make sure that the stage and
the base of the microscope are parallel to each other.
3.
Lock the stage securely with the stage locking
lever.
How to remove the cross-movement device
@
1.
Tu rn the stopper
CD on the back side of the
stage through 90 degrees to disengage the back·
forth movement stopper.
2.
Turn the
turn idle
back·far~h
movement handle ® until it
(the rack and pinion are disengaged).
and then remove the cross· movement device by
pulling it out.
Now the stage can be used is a plain stage.
B. Attach the illuminator
(I~
Fit the illuminator assembly in the mount
upper end of the microscope
projecting tube
downward.
,~/
arm
with
on the
the
light
and the light shielding tube facing
Lock the illuminator in place by turning
the fastening ring
(~).
Ueplacement of lamp
@
1.
By loosening the socket locking knob (I), the
socket
2.
(~;
can be pulled out.
To removed the lamp, slightly press down the
®
lamp and turn it to the left, and then it comes
off easily.
C. Install the microscope head
Fit the microscope head in the microscope head mount on the illuminator body and lock it by turning
the fixing ring.
D. Attach the objectives
Screw the objectives into the respectively marked mounting holes; 5 x lens into A hole, 10 x
into B hole and 40 x lens into C hole.
*
It is suggested that the blind cover is put into D hole to prevent the entry of dust.
E. Insert the eyepiece(s)
F. Connect the cord
1.
Plug the secondary cord
0.)
into the socket lID on
the transformer.
2.
Make sure that the transformer's main switch is
set at OFF and connect the power cord @ to an
electric outlet.
-
8 -
lens
\111. HOW TO USE
A. Adjustment of the movement of coarse
adjustment handle
The coarse adjustment handle is usually heavy and
tight.
This can be adjusted to the user's preference.
Hold right and left handles and turn them in reverse
direction, the handles loosened so that they can be
moved easily.
The movement of the handles will
become heavier when the handles are turned in the
opposite direction.
B. Place the specimen on the stage
Place some oil-clay on the metal slide and the specimen on top of it.
Press them by the hand press,
and place it on the stage in such manner that the specimen surface will be right-angled toward
the optical axis.
Secure the specimen on the stage firmly with the stage clip.
placed on the stage.
Two slides may be
Cross movement of the specimen can be made with the cross-movement hanrll..
on the stage.
C. Centering of the light source lamp
1.
Lower the output voltage of the transformer and set the switch to ON and adjust the brightness
by using the voltage control knob.
The pilot lamp will be lighted when the switch is turned
to ON.
2.
3.
Set the Objective 10 x in place.
Pull out all the way the optical path selector knob on the illuminator (to switch to bright field
illumination) .
4.
Insert the eyepiece(s) and focus the specimen (For the focusing procedures, see per D.)
Use your right eye to look through the right·hand eyepiece when binocular or trinocular type
is used.
5.
Remove the eyepiece and look through the eyepiece tube (the right.hand eyepiece when binocular
or trinocular microscope tube is used) and you will see an image of the lamp filament.
6.
Operate the two centering screws on the lamphouse until the image of filament is seen in the
center of the field.
7.
Imcomplete
centering
Replace the eyepiece.
Now the microscope is ready for use.
Complete
centering
D. Focusing
1.
Turn the fine adjustment handle so that the fine adjustment mark comes halfway between the
fine adjustment range marking lines.
2.
Use the Objective 10 x.
3.
Insert a desired eyepiece(s) into the eyepiece tube(s).
4.
Looking at the specimen from the side, turn the coarse adjustment handle to raise the stage
5.
Looking through the eyepiece(s), turn the coarse adjustment handle to slowly lower the stage
until the surface of specimen comes very close to the tip of objective.
for rough adjustment of focus.
6.
Turn the nosepiece to set a desired objective in place.
7.
Use the fine adjustment handle to make accurate adjustment of focus.
E. Interpupillary Distance and Diopter Adjustments
In order to obtain perfect binocular vision through the eyepieces, it is necessary to adjust interpupillary
distance and diopter difference in eye acuity; otherwise, long time observation would put considerable
strain on the observer's eyes.
1.
Interpupillary Distance Adjustment
1) Hold the right and left eyepiece tubes with both hands and push the tubes together, or pull them
apart lilterally, whichever is required, while looking through the eyepieces with both eyes, until
perfect binocular vision is obtained.
2) Memorize your interpupillary distance setting. Scale
CD
is provided for this purpose, located
between the eyepiece tubes.
*
This interpupillary distance adjustment is necessary each time observers are changed.
Re-focusing is also necessary whenever the interpupillary distance is changed.
2.
Diopter Adjustment
1) Rotate the diopter ring
®
on the right eypiece tube to match the scale on the ring to your
iuterpupillary distance setting which you obtained from
scale
CD
as described in the preceding paragraph 1-(2).
2) Look at the image through the right eyepiece with your right
eye and focus on the specimen with the fine adjustment
knobs.
3) Next, look at the image through the left eyepiece with your
left eye and rotate the diopter ring @ to focus on the specimen without using the coarse and fine adjustment knobs.
F. Bright field and dark field observation
Bright field and dark field can be readily selected by means
of the optical path selector knob
Dark field illumination:
~l '.
The knob is pressed in.
Bright field illumination: The knob is pulled out.
Etched marks are provided for the selector.
D stands for DARK (dark field illumination).
B stands for 13l{IGHT (bright field illumination).
_.....
shows the direction of the movement of the
knob which is pulled out or pushed in.
-
10-
®
G. Oblique illumination
Mount for sector diaphragm
slip the sector diaphragm into the Mount.
1.
2.
An oblique illumination can be obtained by the
following manner. push in the optical path selector
knob to get the dark field. Insert the mount for
sector diaphragm (accessory) into the filter slot of
the illumination tube.
The sector diaphragms are of two kinds. Each of
them consists of two plates so that by rotating
either of them the size of the diaphragm aperture
can be changed to adjust the extent of the area to
be illuminated.
For 275°
H. Observation with polarized light
Observation with polarized light is possible by using the polarizing equipment (polarizer and analyzer),
provided as a special accessory.
Adopt the following procedures for observation with polarized light.
1)
Remove the microscope head (monocular, binocular
and trinocular).
2)
Slowly drop the analyzer in place with its positioning
groove :,1 matched with the positioning pin
I~;
on
the illuminator.
The engraved mark
A on
the
analyzer's frame
stands for ANALYZER.
3)
Replace the microscope head.
4)
Insert the polarizer through the polarizer slot in the
illumination tube.
In inserting the polarizer, make
sure that the engraved mark P faces the scale on the illumination tube.
The engraved mark P stands for POLARIZER.
5)
Parallel and crossed positions can be obtained by rotating the analyzer by a rotating knob on the
illuminator and the polarizer by the polarizer rotating knob.
~
Crossed
1
2
1
Parallel
2
3
*
Polarizer
setting (scale)
Analyzer
setting (scale)
0
90°
45°
0
90°
0
90°
45°
90°
0
Match the • mark with the engraved on scale for the analyzer and match the polarizer knob
with the scale for the polarizer.
I.
Photography
1.
When monocular or binocular head is used, replace
the head
with
the
straight
tube
for
photomicrography.
2.
When trinocular head is used, attach the photographic apparatus to the straight tube and use the
optical path selector knob on the head for photographic work.
If the optical path selector knob is pulled out, the optical path is turned to the straight tube
and it is switched to the binocular tube if the knob is pushed in.
-11-
VIIT. CHARACTERISTICS OF LENSES
Objective
Magnification
Neo 5x
Neo lOx
Neo 40x
NA
0.10
0.25
0.65
WD
22.30
7.50
0.63
Focal Magnifi.
length cation
31.41
Eyepiece
No. of view
field
Depth of
focus
P7x
18
35x
3.6
124.6
13
50x
2.6
95.5
75x
1.9
72.8
9.5
WFI0x
18
50x
3.6
95.5
WF15x
12
75x
2.4
72.8
P7x
18
70x
1.8
23.8
P10x
13
100x
1.3
18.0
150x
0.95
13.5
18.0
P15x
5.39
Actual
field of
view
PI0x
P15x
19.58
Total
magnification
9.5
WF10x
18
100 x
1.8
WF15x
12
150X
1.2
13.5
P7x
18
280 x
0.45
2.5
P10x
13
2.0
P15x
9.5
400x
0.33
600 x
0.24
1.5
WF10x
18
400 x
0.45
2.0
WF15x
12
600 x
0.3
1.5
Actual field of view:
No. of view field:
A number which represents in mm
the size of the image of the field
diaphragm that is formed by the
lens in front of it. (JIS 87132)
Total magnification:
Magnification of objective X magnification of eyepiece.
No. of view field of Eyepiece
Magnification of objective
(mm)
)
Depth of focus:
The extent of the depth of specimen
in focus. (p)
IX. IMPORTANT POINTS TO REMEMBER
Dampness and dust are the worst enemies of a microscope.
Special care must be taken to protect the instrument from the principal trouble makers.
It frequently happens that a research laboratory and other places where microscopes are used have
such unfavorable conditions. So, it is advisable that when the instrument is not in use it is stored
in the case. If working conditions at the laboratory do not allow the equipment to be stored away
every time its job is finished, use the accessory vinyl cover to protect it from dust.
It is best to store eyepieces and objectives in the dessicator.
It is advisable to place silicagel
(dessicating agent) in the container. When the eyepiece is detached from the microscope, the eyepiece
tube should be covered with its cap.
Strictly avoid disassembling and tinkering with the mechanical parts of the microscope. Leave such
work to professional technicians. Great care must be exercised in cleaning the instrument.
For example, when you want to remove dust from inaccessible parts of the instrument. blow the
dust with a rubber blower or carefully brush it away with a soft brush.
-
12-
)
As we are continually improving and developing our products, the equipment supplied may not agree
in all details with the descriptions amI/or illustrations shown in this instructions.