columns IonPac® AS14A Anion-Exchange Column The IonPac AS14A is a high capacity, carbonate-selective anionexchange column designed for the fast, isocratic separation of inorganic anions including fluoride, acetate, chloride, nitrite, bromide, nitrate, phosphate, and sulfate. Separation of the common inorganic anions can be achieved in 8 minutes on the AS14A-5µm (3 x 150 mm) column and 13 minutes on the AS14A-7µm (4 x 250 mm) column. The IonPac AS14A is suited for all IonPac AS4A, AS4A-SC, and AS14 column applications. The AS14A column offers excellent retention of fluoride from the water dip. Solvent compatibility permits easy column clean-up after the analysis of complex matrices. Superior Chromatographic Performance • Fast, isocratic separation of common inorganic anions in 8 minutes (3 x 150 mm) or 13 minutes (4 x 250 mm) using a simple carbonate/bicarbonate eluent • Designed to be used in IonPac AS4A, AS4A-SC, and AS14 applications with equivalent linearity and precision • Meets or exceeds performance requirements of U.S. EPA Method 300.0 (A) • Superior retention and quantification of fluoride • Sodium or potassium hydroxide gradient optimizes difficult separations • Compatible with organic solvents to enhance analyte solubility, modify column selectivity, or facilitate column clean-up • Direct transfer of 4-mm applications to the 3-mm column format for more economical operation, twofold reduction in eluent consumption, and twofold increase in mass sensitivity Determination of Inorganic Anions in Diverse Sample Matrices • Source water and drinking water • Municipal and industrial wastewater • Industrial cooling water • Hazardous waste extracts and dump site leachates • Acid rain • Foods and beverages • Anionic counterions in pharmaceuticals and synthetic peptides • Polymers such as polyols and polysulfonates • Scrubber solutions Inorganic Anions in Drinking Water and Wastewater The IonPac AS14A is ideal for compliance monitoring of drinking water and wastewater. The AS14A meets or exceeds the performance requirements of U.S EPA Method 300.0 (A). The common inorganic anions can be separated in 8 minutes in drinking water as illustrated in Figure 3. Low levels of inorganic anions in wastewater can be determined easily with the AS14A column as shown in Figure 4, even though wastewater can be a more complex sample than drinking water. 100 A 2 Column: IonPac AG14A, AS14A-5µm (3 x 150 mm) Eluent: 8.0 mM Sodium carbonate 1.0 mM Sodium bicarbonate Temperature: 30 °C Flow Rate: µS 7 Detection: 1 3 0 2 5 6 4 0 4 2 Drinking water from Milpitas, CA Peaks: 1. 2. 3. 4. 5. 6. 7. 7 2 B Suppressed conductivity, ASRS-ULTRA 2-mm, AutoSuppression recycle mode Sample: 10 8 6 0.5 mL/min Inj. Volume: 12.5 µL 4 Fluoride Chloride Bromide Nitrate Chlorate Phosphate Sulfate 0.06 mg/L (ppm) 42 0.03 3.2 0.14 1.05 61.0 µS 3 1 5 6 0 0 4 2 8 6 10 Minutes 15721a Figure 3. Determination of inorganic anions in a drinking water sample on an IonPac AS14A-5µm (3 x 150 mm) column. Column: IonPac AG14A, AS14A-5µm (3 x 150 mm) Eluent: 8.0 mM Sodium carbonate 1.0 mM Sodium bicarbonate 8 2 4 Temperature: 30 °C Flow Rate: 0.5 mL/min Inj. Volume: 5 µL µS Detection: 2 5 3 Sample Prep: Diluted 1:100 6 Peaks: 7 1 0 0 2 4 Minutes Suppressed conductivity, ASRS-ULTRA 2-mm, AutoSuppression recycle mode 6 8 10 1. Unknown 2. Acetate 3. Formate 4. Chloride 5. Bromide 6. Nitrate 7. Phosphate 8. Sulfate Figure 4. Determination of inorganic anions in a wastewater sample from a chemical manufacturer on an IonPac AS14A-5µm (3 x 150 mm) column. 15969 Inorganic Anions in Drinking Water and Wastewater The IonPac AS14A is ideal for compliance monitoring of drinking water and wastewater. The AS14A meets or exceeds the performance requirements of U.S EPA Method 300.0 (A). The common inorganic anions can be separated in 8 minutes in drinking water as illustrated in Figure 3. Low levels of inorganic anions in wastewater can be determined easily with the AS14A column as shown in Figure 4, even though wastewater can be a more complex sample than drinking water. 100 A 2 Column: IonPac AG14A, AS14A-5µm (3 x 150 mm) Eluent: 8.0 mM Sodium carbonate 1.0 mM Sodium bicarbonate Temperature: 30 °C Flow Rate: µS 7 Detection: 1 3 0 2 5 6 4 0 4 2 Drinking water from Milpitas, CA Peaks: 1. Fluoride (ppm) 2. Chloride 3. Bromide 4. Nitrate 5. Chlorate 6. Phosphate 7. Sulfate 7 2 B Suppressed conductivity, ASRS-ULTRA 2-mm, AutoSuppression recycle mode Sample: 10 8 6 0.5 mL/min Inj. Volume: 12.5 µL 4 0.06 mg/L 42 0.03 3.2 0.14 1.05 61.0 µS 3 1 5 6 0 0 4 2 8 6 10 Minutes 15721a Figure 3. Determination of inorganic anions in a drinking water sample on an IonPac AS14A-5µm (3 x 150 mm) column. Column: IonPac AG14A, AS14A-5µm (3 x 150 mm) Eluent: 8.0 mM Sodium carbonate 1.0 mM Sodium bicarbonate 8 2 4 Temperature: 30 °C Flow Rate: 0.5 mL/min Inj. Volume: 5 µL µS Detection: 2 5 3 Sample Prep: Diluted 1:100 6 Peaks: 7 1 0 0 2 4 Minutes Suppressed conductivity, ASRS-ULTRA 2-mm, AutoSuppression recycle mode 6 8 10 1. Unknown 2. Acetate 3. Formate 4. Chloride 5. Bromide 6. Nitrate 7. Phosphate 8. Sulfate Figure 4. Determination of inorganic anions in a wastewater sample from a chemical manufacturer on an IonPac AS14A-5µm (3 x 150 mm) column. 15969 12 3 1 Column: IonPac AG14A, AS14A-5µm (3 x 150 mm) Eluent: 8.0 mM Sodium carbonate 1.0 mM Sodium bicarbonate Inj. Volume: 5 µL 8 6 Detection: Suppressed conductivity, ASRS-ULTRA 2-mm, AutoSuppression recycle mode Flow Rate: 0.8 mL/min Temp: 30 ˚C Peaks: 1. 2. 3. 4. 5. 6. 7. 8. 5 4 7 µS 2 0 0 2 1 Versatile Operation The IonPac AS14A-5µm (3 x 150 mm) can be operated at higher flow rates for fast analysis of inorganic anions as shown in Figure 5. Figure 6 illustrates use of the AS14A-5µm (3 x 150 mm) column with a hydroxide gradient for maximized resolution of fluoride, acetate, formate, and glycolate along with the common inorganic anions. 4 3 Minutes 6 5 Fluoride Acetate Chloride Nitrite Bromide Nitrate Phosphate Sulfate 5 mg/L (ppm) 20 10 15 25 25 40 30 15907 Figure 5. The IonPac AS14A-5µm (3 x 150 mm) packing permits fast anion analysis. Column: IonPac AG14A, AS14A-5µm (3 x 150 mm) Eluent: 5 mM KOH for 6 min to 80 mM KOH at 17 min Eluent Source: EG40 30 12 5 µL Inj. Volume: Detection: Suppressed conductivity, ASRS-ULTRA 2-mm, AutoSuppression recycle mode Flow Rate: 0.6 mL/min Temp: 30 ˚C Peaks: 1. Fluoride 2. Glycolate 3. Acetate 4. Formate 5. Chlorite 6. Chloride 7. Bromate 8. Nitrite 9. Bromide 10. Nitrate 11. Chlorate 12. Sulfate 13. Oxalate 14. Phosphate 13 10 µS 14 9 4 1 2 11 6 8 7 3 5 0 0 5 10 Minutes 15 20 5 mg/L (ppm) 10 20 10 10 10 20 15 25 25 25 30 30 40 15921 Figure 6. Potassium hydroxide gradient separation of anions using the IonPac AS14A-5µm (3 x 150 mm) column. A) Anion Standard 16 1 3 µS 2 4 IonPac AG14A, AS14A-5µm (3 x 150 mm) Eluent: 8.0 mM Sodium carbonate 1.0 mM Sodium bicarbonate Temperature: 30 °C Flow Rate: 0.5 mL/min 9 6 Column: 5 Inj. Volume: 5 µL 7 8 Detection: Suppressed conductivity, ASRS-ULTRA 2-mm, AutoSuppression recycle mode 0 Panel A 0 2 4 Minutes 6 10 Peaks: 8 9 10 B) Toothpaste Sample 8 1 µS 5 2 3 1. 2. 3. 4. 5. 6. 7. 8. 9. Fluoride Formate Chloride Nitrite Bromide Nitrate Phosphate Monofluorophosphate Sulfate 5 mg/L (ppm) 10 10 15 25 25 40 50 30 7 6 0 0 2 4 6 8 10 Minutes 15726 Figure 7. Determination of anionic additives in toothpaste on an IonPac AS14A-5µm (3 x 150 mm) column. Extended Application Capabilities The unique selectivity and high capacity of the AS14A column make it an ideal column for methods development of specialized anion applications. For example, anionic additives in personal care products can easily be determined. The AS14A provides excellent separation of monofluorophosphate, phosphate, and sulfate found in dental care products in less than 10 minutes using a standard carbonate/bicarbonate eluent as illustrated in Figure 7. The IonPac AS14A also is ideal for the determination of anions in food and beverage samples containing high concentrations of carbonate. Carbonated bottled water can contain up to 500 mg/L (ppm) of carbonate but requires only minimal sample preparation (dilution and sonication) prior to analysis. Figure 8 illustrates the determination of inorganic anions in carbonated water using the AS14A column. Column: IonPac AG14A, AS14A-7µm (4 x 250 mm) Eluent: 8.0 mM Sodium carbonate 1.0 mM Sodium bicarbonate Temperature: 30 ˚C Flow Rate: 1.0 mL/min Inj. Volume: 10 µL Detection: Suppressed conductivity, ASRS-ULTRA 4-mm, AutoSuppression recycle mode Peaks: 1. 2. 3. 4. 5. 180 180 2 2 Sample Prior to Sonication Sample After Sonication µS µS Unknown Chloride Bromide Nitrate Sulfate 5 Ordering Information For optimum ease of use and economy, the IonPac AS14A column can be used with either the ASRS Anion Self-Regenerating Suppressor or the Anion Atlas Electrolytic Suppressor. For additional ease of use, Dionex offers an AS14A Eluent Concentrate and a line of anion standards. Refer to the part numbers that follow or see the Dionex Product Selection Guide for more details. When performing gradient anionexchange applications using hydroxide eluents on the AS14A, an Anion Trap Column (ATC-3) should be installed between the gradient pump and the injection valve to remove anionic contaminants from the eluent. For 4-mm concentrator work, use the IonPac AG14A 4-mm guard column, TAC Anion Concentrator Column, or AMC-1 Anion MicroConcentrator when a single-piston pump such as the DQP or DXP is used for sample delivery. Use the TAC-LP1 Anion Concentrator Column when the sample is delivered with a syringe or with a low-pressure autosampler such as the AS40. For 3-mm concentrator work, use the IonPac AG14A 4-mm guard column or the AMC-1 Anion MicroConcentrator when a singlepiston pump such as the DQP or DXP 5 3 4 1 1 3 4 0 0 5 0 2 10 2 0 15 5 2 5 µS 10 15 Minutes 2 5 µS 0 0 3 4 1 3 4 1 0 10 5 15 0 10 5 15 Minutes Minutes 15725 Figure 8. Determination of inorganic anions in carbonated water on an IonPac AS14A-7µm (4 x 250 mm) column. S P E C I F I C AT I O N S Dimensions: Analytical Column: 3 x 150 mm or 4 x 250 mm Guard Column: 3 x 30 mm or 4 x 50 mm Maximum Operating Pressure: 4000 psi Mobile Phase Compatibility: pH 0–14; 0–100% HPLC solvents Substrate Characteristics: Bead Diameter: 5 µm (3 x 150 mm) 7 µm (4 x 250 mm) Pore Size: 100 Å Crosslinking (%DVB): 55% Ion-Exchange Group: Surface-functionalized alkyl quaternary ammonium ion Functional Group Characteristics: Hydrophobicity: Moderate Capacity: 40 µeq (3 x 150 mm analytical column) 8 µeq (3 x 30 mm guard column) 120 µeq (4 x 250 mm analytical column) 24 µeq (4 x 50 mm guard column) Column Construction: PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. O R D E R I N G I N F O R M AT I O N In the U.S., call 1-800-346-6390 or contact the Dionex regional office nearest you. Outside the U.S., order through your local Dionex office or distributor. Refer to the following part numbers. Description Part Number IonPac AS14A Columns IonPac AS14A-7µm Analytical Column (4 x 250 mm) .......................................................................................... 056904 IonPac AG14A-7µm Guard Column (4 x 50 mm) ............................................................................................ 056897 IonPac AS14A-5µm Analytical Column (3 x 150 mm) .......................................................................................... 056901 IonPac AG14A-5µm Guard Column (3 x 30 mm) ............................................................................................ 056899 Eluent Concentrate AS14A Sodium Carbonate/Bicarbonate Eluent Concentrate (250 mL of 100X concentrate) ............................................................... 056937 Trap Columns ATC-3 Anion Trap Column (for use with 4-mm columns) ................................................................ 059660 ATC-3 (2-mm) Anion Trap Column (for use with 3-mm columns) ................................................................ 079932 059661 Concentrator Columns TAC-2 Trace Anion Concentrator (3 x 35 mm) ............................................................................................ 043101 TAC-LP1 Trace Anion Concentrator (4 x 35 mm) ............................................................................................ 046026 AMC-1 Anion MicroConcentrator (2 x 15 mm) ............................................................................................ 051760 Self-Regenerating Suppressor, IonPac, and ASRS are registered trademarks of Dionex Corporation. Printed on recycled and recyclable paper. Dionex Corporation 1228 Titan Way P.O. Box 3603 Sunnyvale, CA 94088-3603 (408) 737-0700 Dionex Corporation Salt Lake City Technical Center 1515 West 2200 South, Suite A Salt Lake City, UT 84119-1484 (801) 972-9292 Dionex U.S. Regional Offices Sunnyvale, CA (408) 737-8522 Westmont, IL (630) 789-3660 Houston, TX (281) 847-5652 Atlanta, GA (770) 432-8100 Marlton, NJ (856) 596-0600 Dionex International Subsidiaries Austria (01) 616 51 25 Belgium (015) 203800 Canada (905) 844-9650 China (852) 2428 3282 Denmark (45) 36 36 90 90 France 01 39 46 08 40 Germany 06126-991-0 Italy (06) 66 51 50 52 Japan (06) 6885-1213 The Netherlands (0161) 43 43 03 Switzerland (062) 205 99 66 United Kingdom (01276) 691722 * Designed, developed, and manufactured under an NSAI registered ISO 9001 Quality System. LPN 1213-02 3M 7/02 © 2002 Dionex Corporation
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