columns
IonPac® AS14A Anion-Exchange Column
The IonPac AS14A is a high
capacity, carbonate-selective anionexchange column designed for the fast,
isocratic separation of inorganic
anions including fluoride, acetate,
chloride, nitrite, bromide, nitrate,
phosphate, and sulfate. Separation of
the common inorganic anions can
be achieved in 8 minutes on the
AS14A-5µm (3 x 150 mm) column
and 13 minutes on the AS14A-7µm
(4 x 250 mm) column. The IonPac
AS14A is suited for all IonPac AS4A,
AS4A-SC, and AS14 column applications. The AS14A column offers
excellent retention of fluoride from
the water dip. Solvent compatibility
permits easy column clean-up after the
analysis of complex matrices.
Superior Chromatographic Performance
•
Fast, isocratic separation of common inorganic anions in 8 minutes
(3 x 150 mm) or 13 minutes
(4 x 250 mm) using a simple
carbonate/bicarbonate eluent
•
Designed to be used in IonPac
AS4A, AS4A-SC, and AS14
applications with equivalent
linearity and precision
•
Meets or exceeds performance
requirements of U.S. EPA Method
300.0 (A)
•
Superior retention and quantification
of fluoride
•
Sodium or potassium hydroxide
gradient optimizes difficult separations
•
Compatible with organic solvents to
enhance analyte solubility, modify
column selectivity, or facilitate
column clean-up
•
Direct transfer of 4-mm applications
to the 3-mm column format for more
economical operation, twofold
reduction in eluent consumption, and
twofold increase in mass sensitivity
Determination of Inorganic Anions in
Diverse Sample Matrices
•
Source water and drinking water
•
Municipal and industrial wastewater
•
Industrial cooling water
•
Hazardous waste extracts and dump
site leachates
•
Acid rain
•
Foods and beverages
•
Anionic counterions in pharmaceuticals and synthetic peptides
•
Polymers such as polyols and
polysulfonates
•
Scrubber solutions
Inorganic Anions in Drinking Water and
Wastewater
The IonPac AS14A is ideal for
compliance monitoring of drinking
water and wastewater. The AS14A
meets or exceeds the performance
requirements of U.S EPA Method
300.0 (A). The common inorganic
anions can be separated in 8 minutes in
drinking water as illustrated in Figure
3. Low levels of inorganic anions in
wastewater can be determined easily
with the AS14A column as shown in
Figure 4, even though wastewater can
be a more complex sample than
drinking water.
100
A
2
Column:
IonPac AG14A, AS14A-5µm
(3 x 150 mm)
Eluent:
8.0 mM Sodium carbonate
1.0 mM Sodium bicarbonate
Temperature: 30 °C
Flow Rate:
µS
7
Detection:
1
3
0
2
5 6
4
0
4
2
Drinking water from Milpitas, CA
Peaks:
1.
2.
3.
4.
5.
6.
7.
7
2
B
Suppressed conductivity,
ASRS-ULTRA 2-mm,
AutoSuppression recycle mode
Sample:
10
8
6
0.5 mL/min
Inj. Volume: 12.5 µL
4
Fluoride
Chloride
Bromide
Nitrate
Chlorate
Phosphate
Sulfate
0.06 mg/L (ppm)
42
0.03
3.2
0.14
1.05
61.0
µS
3
1
5 6
0
0
4
2
8
6
10
Minutes
15721a
Figure 3. Determination of inorganic anions in a drinking water sample on an IonPac
AS14A-5µm (3 x 150 mm) column.
Column:
IonPac AG14A, AS14A-5µm
(3 x 150 mm)
Eluent:
8.0 mM Sodium carbonate
1.0 mM Sodium bicarbonate
8
2
4
Temperature: 30 °C
Flow Rate:
0.5 mL/min
Inj. Volume: 5 µL
µS
Detection:
2
5
3
Sample Prep: Diluted 1:100
6
Peaks:
7
1
0
0
2
4
Minutes
Suppressed conductivity,
ASRS-ULTRA 2-mm,
AutoSuppression recycle mode
6
8
10
1. Unknown
2. Acetate
3. Formate
4. Chloride
5. Bromide
6. Nitrate
7. Phosphate
8. Sulfate
Figure 4. Determination of inorganic anions in a wastewater sample from a chemical
manufacturer on an IonPac AS14A-5µm (3 x 150 mm) column.
15969
Inorganic Anions in Drinking Water and
Wastewater
The IonPac AS14A is ideal for
compliance monitoring of drinking
water and wastewater. The AS14A
meets or exceeds the performance
requirements of U.S EPA Method
300.0 (A). The common inorganic
anions can be separated in 8 minutes in
drinking water as illustrated in Figure
3. Low levels of inorganic anions in
wastewater can be determined easily
with the AS14A column as shown in
Figure 4, even though wastewater can
be a more complex sample than
drinking water.
100
A
2
Column:
IonPac AG14A, AS14A-5µm
(3 x 150 mm)
Eluent:
8.0 mM Sodium carbonate
1.0 mM Sodium bicarbonate
Temperature: 30 °C
Flow Rate:
µS
7
Detection:
1
3
0
2
5 6
4
0
4
2
Drinking water from Milpitas, CA
Peaks:
1. Fluoride
(ppm)
2. Chloride
3. Bromide
4. Nitrate
5. Chlorate
6. Phosphate
7. Sulfate
7
2
B
Suppressed conductivity,
ASRS-ULTRA 2-mm,
AutoSuppression recycle mode
Sample:
10
8
6
0.5 mL/min
Inj. Volume: 12.5 µL
4
0.06 mg/L
42
0.03
3.2
0.14
1.05
61.0
µS
3
1
5 6
0
0
4
2
8
6
10
Minutes
15721a
Figure 3. Determination of inorganic anions in a drinking water sample on an IonPac
AS14A-5µm (3 x 150 mm) column.
Column:
IonPac AG14A, AS14A-5µm
(3 x 150 mm)
Eluent:
8.0 mM Sodium carbonate
1.0 mM Sodium bicarbonate
8
2
4
Temperature: 30 °C
Flow Rate:
0.5 mL/min
Inj. Volume: 5 µL
µS
Detection:
2
5
3
Sample Prep: Diluted 1:100
6
Peaks:
7
1
0
0
2
4
Minutes
Suppressed conductivity,
ASRS-ULTRA 2-mm,
AutoSuppression recycle mode
6
8
10
1. Unknown
2. Acetate
3. Formate
4. Chloride
5. Bromide
6. Nitrate
7. Phosphate
8. Sulfate
Figure 4. Determination of inorganic anions in a wastewater sample from a chemical
manufacturer on an IonPac AS14A-5µm (3 x 150 mm) column.
15969
12
3
1
Column:
IonPac AG14A, AS14A-5µm
(3 x 150 mm)
Eluent:
8.0 mM Sodium carbonate
1.0 mM Sodium bicarbonate
Inj. Volume: 5 µL
8
6
Detection:
Suppressed conductivity,
ASRS-ULTRA 2-mm,
AutoSuppression recycle mode
Flow Rate:
0.8 mL/min
Temp:
30 ˚C
Peaks:
1.
2.
3.
4.
5.
6.
7.
8.
5
4
7
µS
2
0
0
2
1
Versatile Operation
The IonPac AS14A-5µm
(3 x 150 mm) can be operated at higher
flow rates for fast analysis of inorganic
anions as shown in Figure 5.
Figure 6 illustrates use of the
AS14A-5µm (3 x 150 mm) column
with a hydroxide gradient for maximized resolution of fluoride, acetate,
formate, and glycolate along with the
common inorganic anions.
4
3
Minutes
6
5
Fluoride
Acetate
Chloride
Nitrite
Bromide
Nitrate
Phosphate
Sulfate
5 mg/L (ppm)
20
10
15
25
25
40
30
15907
Figure 5. The IonPac AS14A-5µm (3 x 150 mm) packing permits fast anion analysis.
Column:
IonPac AG14A, AS14A-5µm (3 x 150 mm)
Eluent:
5 mM KOH for 6 min
to 80 mM KOH at 17 min
Eluent Source: EG40
30
12
5 µL
Inj. Volume:
Detection:
Suppressed conductivity,
ASRS-ULTRA 2-mm,
AutoSuppression recycle mode
Flow Rate:
0.6 mL/min
Temp:
30 ˚C
Peaks:
1. Fluoride
2. Glycolate
3. Acetate
4. Formate
5. Chlorite
6. Chloride
7. Bromate
8. Nitrite
9. Bromide
10. Nitrate
11. Chlorate
12. Sulfate
13. Oxalate
14. Phosphate
13
10
µS
14
9
4
1
2
11
6 8
7
3
5
0
0
5
10
Minutes
15
20
5 mg/L (ppm)
10
20
10
10
10
20
15
25
25
25
30
30
40
15921
Figure 6. Potassium hydroxide gradient separation of anions using the IonPac AS14A-5µm
(3 x 150 mm) column.
A) Anion Standard
16
1
3
µS
2
4
IonPac AG14A, AS14A-5µm (3 x 150 mm)
Eluent:
8.0 mM Sodium carbonate
1.0 mM Sodium bicarbonate
Temperature: 30 °C
Flow Rate: 0.5 mL/min
9
6
Column:
5
Inj. Volume: 5 µL
7
8
Detection:
Suppressed conductivity,
ASRS-ULTRA 2-mm,
AutoSuppression recycle mode
0
Panel A
0
2
4
Minutes
6
10 Peaks:
8
9
10
B) Toothpaste Sample
8
1
µS
5
2 3
1.
2.
3.
4.
5.
6.
7.
8.
9.
Fluoride
Formate
Chloride
Nitrite
Bromide
Nitrate
Phosphate
Monofluorophosphate
Sulfate
5 mg/L (ppm)
10
10
15
25
25
40
50
30
7
6
0
0
2
4
6
8
10
Minutes
15726
Figure 7. Determination of anionic additives in toothpaste on an IonPac AS14A-5µm
(3 x 150 mm) column.
Extended Application Capabilities
The unique selectivity and high
capacity of the AS14A column make it
an ideal column for methods development of specialized anion applications.
For example, anionic additives in
personal care products can easily be
determined. The AS14A provides
excellent separation of monofluorophosphate, phosphate, and sulfate
found in dental care products in less
than 10 minutes using a standard
carbonate/bicarbonate eluent as
illustrated in Figure 7.
The IonPac AS14A also is ideal
for the determination of anions in food
and beverage samples containing high
concentrations of carbonate. Carbonated
bottled water can contain up to
500 mg/L (ppm) of carbonate but
requires only minimal sample preparation (dilution and sonication) prior to
analysis. Figure 8 illustrates the determination of inorganic anions in carbonated
water using the AS14A column.
Column:
IonPac AG14A, AS14A-7µm
(4 x 250 mm)
Eluent:
8.0 mM Sodium carbonate
1.0 mM Sodium bicarbonate
Temperature:
30 ˚C
Flow Rate:
1.0 mL/min
Inj. Volume:
10 µL
Detection:
Suppressed conductivity,
ASRS-ULTRA 4-mm,
AutoSuppression recycle mode
Peaks:
1.
2.
3.
4.
5.
180
180
2
2
Sample Prior
to Sonication
Sample After
Sonication
µS
µS
Unknown
Chloride
Bromide
Nitrate
Sulfate
5
Ordering Information
For optimum ease of use and
economy, the IonPac AS14A column
can be used with either the ASRS
Anion Self-Regenerating Suppressor or
the Anion Atlas Electrolytic Suppressor. For additional ease of use, Dionex
offers an AS14A Eluent Concentrate
and a line of anion standards. Refer to
the part numbers that follow or see the
Dionex Product Selection Guide for
more details.
When performing gradient anionexchange applications using hydroxide
eluents on the AS14A, an Anion Trap
Column (ATC-3) should be installed
between the gradient pump and the
injection valve to remove anionic
contaminants from the eluent.
For 4-mm concentrator work, use
the IonPac AG14A 4-mm guard column,
TAC Anion Concentrator Column, or
AMC-1 Anion MicroConcentrator
when a single-piston pump such as the
DQP or DXP is used for sample
delivery. Use the TAC-LP1 Anion
Concentrator Column when the sample
is delivered with a syringe or with a
low-pressure autosampler such as the
AS40. For 3-mm concentrator work,
use the IonPac AG14A 4-mm guard
column or the AMC-1 Anion
MicroConcentrator when a singlepiston pump such as the DQP or DXP
5
3 4
1
1
3 4
0
0
5
0
2
10
2
0
15
5
2
5
µS
10
15
Minutes
2
5
µS
0
0
3 4
1
3 4
1
0
10
5
15
0
10
5
15
Minutes
Minutes
15725
Figure 8. Determination of inorganic anions in carbonated water on an IonPac AS14A-7µm
(4 x 250 mm) column.
S P E C I F I C AT I O N S
Dimensions:
Analytical Column:
3 x 150 mm or 4 x 250 mm
Guard Column:
3 x 30 mm or 4 x 50 mm
Maximum Operating Pressure:
4000 psi
Mobile Phase Compatibility:
pH 0–14; 0–100% HPLC solvents
Substrate Characteristics:
Bead Diameter: 5 µm (3 x 150 mm)
7 µm (4 x 250 mm)
Pore Size: 100 Å
Crosslinking (%DVB): 55%
Ion-Exchange Group:
Surface-functionalized alkyl
quaternary ammonium ion
Functional Group Characteristics:
Hydrophobicity: Moderate
Capacity:
40 µeq (3 x 150 mm analytical column)
8 µeq (3 x 30 mm guard column)
120 µeq (4 x 250 mm analytical column)
24 µeq (4 x 50 mm guard column)
Column Construction:
PEEK with 10-32 threaded ferrule-style
end fittings. All components are nonmetallic.
O R D E R I N G I N F O R M AT I O N
In the U.S., call 1-800-346-6390 or contact the Dionex regional office nearest
you. Outside the U.S., order through your local Dionex office or distributor.
Refer to the following part numbers.
Description
Part Number
IonPac AS14A Columns
IonPac AS14A-7µm Analytical Column
(4 x 250 mm) .......................................................................................... 056904
IonPac AG14A-7µm Guard Column
(4 x 50 mm) ............................................................................................ 056897
IonPac AS14A-5µm Analytical Column
(3 x 150 mm) .......................................................................................... 056901
IonPac AG14A-5µm Guard Column
(3 x 30 mm) ............................................................................................ 056899
Eluent Concentrate
AS14A Sodium Carbonate/Bicarbonate Eluent Concentrate
(250 mL of 100X concentrate) ............................................................... 056937
Trap Columns
ATC-3 Anion Trap Column
(for use with 4-mm columns) ................................................................ 059660
ATC-3 (2-mm) Anion Trap Column
(for use with 3-mm columns) ................................................................ 079932
059661
Concentrator Columns
TAC-2 Trace Anion Concentrator
(3 x 35 mm) ............................................................................................ 043101
TAC-LP1 Trace Anion Concentrator
(4 x 35 mm) ............................................................................................ 046026
AMC-1 Anion MicroConcentrator
(2 x 15 mm) ............................................................................................ 051760
Self-Regenerating Suppressor, IonPac,
and ASRS are registered trademarks of Dionex Corporation.
Printed on recycled and recyclable paper.
Dionex Corporation
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P.O. Box 3603
Sunnyvale, CA
94088-3603
(408) 737-0700
Dionex Corporation
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Salt Lake City, UT
84119-1484
(801) 972-9292
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(630) 789-3660
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(770) 432-8100
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(856) 596-0600
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* Designed, developed, and manufactured under an NSAI registered ISO 9001 Quality System.
LPN 1213-02 3M 7/02
© 2002 Dionex Corporation