2012 Doktora Programı Mezunları Özel Sayısı / Special Edition on PhD Thesis Abstracts of 2012 Graduates MÜSBED 2013;3(Suppl. 2):S61 Evaluation of Gingival Crevicular Fluid Transforming Growth Factor-ß1 and Alkaline Phosphatase Levels after Treatment of Intrabony Periodontal Defects by the Use of Enamel Matrix Derivatives with Autogenous Bone Graft Ömer Birkan Ağralı, Leyla Kuru Marmara University, Faculty of Dentistry, Department of Periodontology, İstanbul Objective: The present study aimed to evaluate the effects of enamel matrix derivatives (EMD) combined with autogenous bone graft (ABG) application on clinical and radiographic parameters and gingival crevicular fluid (GCF) transforming growth factor-β1 (TGF-β1) and alkaline phosphatase (ALP) levels in chronic periodontitis patients with intrabony defects and, to compare with EMD alone and open flap debridement (OFD) procedures. Methods: A total of 30 deep intrabony defects in 12 patients were randomly treated with EMD+ABG, EMD alone or OFD. Clinical parameters including plaque index, gingival index, bleeding on probing, probing depth, relative attachment level and recession were recorded at baseline and 6 months post-surgery. Intrabony defect fill (DF) percentage was calculated on the standardized radiographs. TGF-β1 and ALP levels were evaluated in GCF just before surgery and 7, 14, 30, 90, 180 days after surgery using ELISA. Results: All treatment procedures led to significant improvements at 6 months follow-up period (p<0.01). Gain in attachment level (p<0.001) and DF (p<0.05) of the EMD+ABG and EMD groups were found to be significantly higher than those of the OFD group, while the use of EMD either with ABG or alone was observed to produce significantly less recession than the OFD (p<0.05). Furthermore, no significant differences were present between the EMD+ABG and EMD groups in terms of the clinical, radiographic and biochemical parameters. Also, no significant differences were present between all three groups biochemically. Conclusion: GCF, TGF-β1 and ALP levels were increased at the early healing phase but not affected from EMD usage. Key words: Autogenous bone graft, chronic periodontitis, enamel matrix derivatives, gingival crevicular fluid, periodontal flap surgery Marmara Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi Cilt: 3, Özel Sayı: 2, 2013 / Journal of Marmara University Institute of Health Sciences Volume: 3, Supplement: 2, 2013 - http://musbed.marmara.edu.tr S61