Welcome Address We are honoured and pleased to welcome over 200 participants and guests from eighteen different Countries, at the International Symposium on the European outbreak of Xylella fastidiosa in olive, held in Apulia, Italy. The Scientific Committee with the collaboration on the invited speakers have built a scientific program which will provide an in depth and updated overview on this harmful pathogen, and illustrate the current situation in the outbreak area of Apulia. The researchers directly involved in the ongoing research activities on this new emerging threat will share, the knowledge and the data so far gathered, with the international scientific community and critically discuss the future steps and efforts to boost the ongoing research programs. As a complement to the core scientific program, the National and the Regional Plant Health Services have organized a specific session for the presentation and discussion of the phytosanitary and legislative measures to be adopted and implemented. The Symposium offers, with the field trip, a unique chance to visit the diseased area and have a look of the incidence and severity of the symptoms. We are delighted to invite a broad scientific community to this symposium. We very much hope that you will share our enthusiasm for this Symposium and we thank everybody who have decided to join us in this beautiful area. The Organizing Committee 2 Congress Board SCIENTIFIC COMMITTEE Giovanni Paolo MARTELLI EDITORIAL GRAPHIC DESIGNER Massimiliano MORELLI Rodrigo ALMEIDA WEBMASTERS Simona ABBÀ CNR Institute for Sustainable Plant Protection, Bari, Italy University of Bari Aldo Moro, Bari, Italy University of California, Berkeley, USA Domenico BOSCO CNR Institute for Sustainable Plant Protection, Torino, Italy Jacob Dirk JANSE CNR Institute for Sustainable Plant Protection, Bari, Italy Pasquale SALDARELLI University of Turin, Italy COLLABORATORS Oriana POTERE Giovanna BOTTALICO Daniele CORNARA Leonardo SUSCA Antonio CEGLIE Dutch Inspection Service, Emmeloord, The Netherlands Alexander H. PURCELL University of California, Berkeley, USA ORGANIZING COMMITTEE Donato BOSCIA CNR Institute for Sustainable Plant Protection, Bari, Italy University of Bari Aldo Moro, Bari, Italy Michele DIGIARO Michela CHIUMENTI Angelo DE STRADIS CIHEAM Mediterranean Agronomic Institute, Bari, Italy Bruno Caio FARAGLIA CNR Institute for Sustainable Plant Protection, Bari, Italy Ministry of Agricultural, Food and Forestry Policies, Italy Toufic ELBEAINO Franco VALENTINI Peter MOUBARAK Antonio GUARIO Regional Phytosanitary Service, Apulia, Italy Franco NIGRO CIHEAM Mediterranean Agronomic Institute, Bari, Italy University of Bari Aldo Moro, Bari, Italy Antonella SAPONARI Leonardo CALAPRICO Leonardo CATUCCI Maria FASANO Milena GUIDA Vito MONTILON Donato PALMISANO Milena PETRUZZI Maria PINTO Paola POLLASTRO Maria Rosaria SILLETTI Nicola TRISCIUZZI Giuseppe ALTAMURA Francesco PORCELLI University of Bari Aldo Moro, Bari, Italy Vito Nicola SAVINO University of Bari Aldo Moro, Bari, Italy CONGRESS SECRETARIAT Giuliana LOCONSOLE CNR Institute for Sustainable Plant Protection, Bari, Italy Francesco PALMISANO CRSFA “Basile Caramia”, Locorotondo (Bari), Italy Maria SAPONARI CNR Institute for Sustainable Plant Protection, Bari, Italy ADMINISTRATIVE SECRETARIAT Grazia Valentina TRITTO CNR Institute for Sustainable Plant Protection, Bari, Italy CRSFA “Basile Caramia”, Locorotondo (Bari), Italy Maria Grazia PIEPOLI CRSFA “Basile Caramia”, Locorotondo (Bari), Italy 3 Acknowledgements The INTERNATIONAL SYMPOSIUM ON THE EUROPEAN OUTBREAK OF XYLELLA FASTIDIOSA IN OLIVE has been organized by: CNR INSTITUTE FOR SUSTAINABLE PLANT PROTECTION, BARI, ITALY DEPARTMENT OF SOIL, PLANT AND FOOD SCIENCE, UNIVERSITY OF BARI ALDO MORO, BARI, ITALY CRSFA RESEARCH CENTER “BASILE CARAMIA”, LOCOROTONDO (BARI), ITALY CIHEAM MEDITERRANEAN AGRONOMIC INSTITUTE, BARI, ITALY The Organizing Committee would like to thank the following Italian institutions which have provided funds to develop this event: MINISTERO PER LE POLITICHE AGRICOLE ALIMENTARI E FORESTALI REGIONE PUGLIA–AREA POLITICHE PER LO SVILUPPO RURALE-SERVIZIO AGRICOLTURA-OSSERVATORIO FITOSANITARIO REGIONALE The Organizing Committee wishes to acknowledge the invited speaker for their invaluable scientific contributions and Prof. G. P. Martelli for precious help with abstract editing. 4 The Organizing Committee wishes to thank the following organizations for their generous ongoing support: GOLD SPONSORS SILVER SPONSORS BRONZE SPONSORS The Organizing Committee is also grateful to the following organization for their kind participation: 5 Key Information CONGRESS SECRETARIAT Dr. Maria Saponari, Dr. Giuliana Loconsole, Dr. Francesco Palmisano Mail to: [email protected]. During the congress, the Secretariat can be reached at +39.3403424074 CONGRESS VENUES Symposium sessions Costa Brada Luxury Resort, Litoranea Santa Maria di Leuca, Gallipoli (Lecce) Web: www.costabradaresort.it | Tel: +39.0833.202551 | Mail to: [email protected] Workshop sessions CRSFA Centro di Ricerca, Sperimentazione e Formazione in Agricoltura “Basile Caramia” Via Cisternino, 281, Locorotondo (Bari) Web: www.crsfa.it | Tel. +39.080.4313071 | Mail to: [email protected] WEB SITE The Congress official website can be reached at: www.ivv.cnr.it/symposium-xylella-fastidiosa ACCREDITATION PROCEDURES Symposium sessions are open to all registered participants. Your ID badge included in delegate‟s bags is required for admission to the Congress Centre and all Congress events. All enquiries should be directed to the registration helpdesk in the entrance hall. Registration Opening Hours: Monday 20th October 17:00−19:00 | Tuesday 21st October 8:30−18:30 | Wednsday 22 nd October 8:30−12:30. CERTIFICATE OF ATTENDANCE Certificate of Attendance will be included in the delegate‟s bag. LANGUAGE English is the Congress official language. Simultaneous tanslation will be available. INTERNET ACCESS General Wi-Fi access is available throughout the Congress Venue. Access password will be provided upon your request. 6 POSTERS AND ORAL PRESENTATIONS Posters will be on display during symposium sessions in the dedicated area. Poster presenters are asked to mount their poster before the opening ceremony. Posters must be removed at the end of symposium sessions. Any posters remaining after this time will be removed by the organisers and cannot be reclaimed. Power Point Presentations, on USB flash drives, should be handed to the representative of the Congress Secretariat in advance but not later than 15 min prior to the start of the session. CONGRESS SOCIAL DINNER Congress Dinner will take place on Tuesday 21st October at the Masseria Li Sauli, Gallipoli (www.lisauli.it). Participants will be asked to present tickets found in delegate‟s bag upon arrival at the event venue. FIELD TRIP During field trip, scheduled on Wednesday 22 nd October (14:00-17:00) participants will have opportunity to visit olive groves in the area were the main Xylella fastidiosa outbreak was first reported in October 2013. Please inform the Congress Secretariat if you are not willing to attend the field trip. SHUTTLE BUS SERVICE Complimentary shuttle bus service from Brindisi/Bari airports to Gallipoli and viceversa will be available for participants attending the Symposium during its entire duration. Please at the airport look for the sign “Xylella”. On Thursday 23 October a shuttles bus will be available from Gallipoli to CRSFA Research Center (Locorotondo) for participants attending workshop training sessions. On Friday 24th October, and upon kind request on Saturday 25th October, shuttle bus service will accompany participants leaving workshop sessions to Brindisi/Bari airports. 7 Touristic Tips CONGRESS LOCATIONS SYMPOSIUM LOCATION:GALLIPOLI (LECCE) Located on the southeastern coast of the „‟Italian heel‟‟, Gallipoli is celebrated for its crystal clear blue sea and the sandy beaches that make its territory one of the most cherished natural attractions in Salento. When viewed from the sea, Gallipoli‟s white skyline reveals itself in all of its oriental beauty, resembling a village of the Cyclades islands. The old town, perched on a limestone islet, is connected to the mainland via an arched bridge. The walls, ramparts and towers that once defended the city from foreign raiders, now provide shelter from the rough sea and confer the city a special charm that takes visitors back in time. Don‟t miss to visit the baroque cathedral dedicated to Saint Agatha and the Hellenistic fountain close to the bridge, rich in bas-reliefs depicting scenes from Greek mythology, and the Sanctuary of Our Lady of Canneto. The city‟s fascination adds to its beautiful nature, offering long beaches of very fine white sand that are mostly cherished by family vacationers and water sports fans. 8 WORKSHOP LOCATION: LOCOROTONDO (BARI) The village is located in Valle d‟Itria and you can enjoy the typical rural landscape from various sight of the historic centre with his small “trulli” locally called “casedde” and farms. A center of concentric streets, low houses with the typical pointed roof and whitewashed, originally built to fight against the sun and the pestilence, and then become the symbol of the country, the “cummerse“. Entering at the city centre by Napoli‟s door, you are immediately greeted by an efficient tourist information service, where you can find all the information materials, in various languages, to better organize your visit. Then you go in the nice built-up area made pleasant by the open spaces and by the white houses with main entrance and railings profiles stone. The food and wine is strongly linked to the local tradition: the white wine Locorotondo DOC and oil, and dishes such as orecchiette with broccoli and the mashed fava beans with wild chicory. In the midway between Cisterino and Alberobello, three are the stage unmissable (all certified with the Orange flag by Touring Club Italiano) a cultural and culinary excellence itinerary through the Itria valley. 9 PROGRAMME SCHEDULE SYMPOSIUM SESSIONS Monday, 20th October 17:00-19:00 REGISTRATION AND POSTER HANGING PROCEDURES 19:00-21:00 WELCOME RECEPTION AT COSTA BRADA RESORT, GALLIPOLI (LECCE) Tuesday, 21st October 08:30-09:00 REGISTRATION AND POSTER HANGING PROCEDURES 09:00-09:30 WELCOME G. P. Martelli, Professor Emeritus, Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Italy M. Martina, Italian Ministy of Agricultural and Forestry Policies N. Vendola, President of the Apulia Regional Government F. Nardoni, Regional Assessor for Agriculture G. P. Accotto, Director of the CNR Institute for Sustainable Plant Protection A. Uricchio, Rector of the Aldo Moro University of Bari C. Lacirignola, General Secretary of the CIHEAM Mediterranean Agronomic Institute, Bari A.Palmisano, President of the CRSFA Research Center “B. Caramia”, Locorotondo (Bari) SESSION I 09:30-10:00 10:00-10:30 10:30-10:50 XYELLA FASTIDIOSA: THE PATHOGEN, ITS EPIDEMIOLOGY AND MANAGEMENT Chairman: Prof. G. P. Martelli HISTORICAL PERSPECTIVES ON XYLELLA FASTIDIOSA AND THEIR RELEVANCE FOR THE FUTURE A. H. Purcell, Professor Emeritus, University of California, Berkeley, USA DISEASES INDUCED BY XYLELLA FASTIDIOSA SUBSP. PAUCA: ECOLOGY, EPIDEMIOLOGY AND MANAGEMENT H. Della Coletta Filho, Centro de Citricultura Sylvio Moreira, IAC Cordeirópolis, São Paulo, Brazil POTENTIAL VECTORS OF XYLELLA FASTIDIOSA IN EUROPE D. Bosco, R. Almeida, E. Czwienczek, G. Stancanelli, J. C. Gregoire, D. Caffier, G. Hollo, C. Bragard 11 10:50-11:10 COFFEE BREAK 11:10-11:40 CONTROL STRATEGIES FOR XYLELLA FASTIDIOSA 11:40-12:10 D. L. Hopkins, University of Florida, Central Florida Research and Education Center, USA CELL DENSITY-DEPENDENT BEHAVIORS OF XYLELLA FASTIDIOSA: ACHIEVING DISEASE CONTROL VIA PATHOGEN CONFUSION S. E. Lindow, University of California, Berkeley, USA 12:10-12:30 SOME APPROACHES AIMING CITRUS VARIEGATED CHLOROSIS CONTROL IN BRAZIL SESSION II OLIVE QUICK DECLINE SYNDROME AND XYLELLA FASTIDIOSA EPIDEMICS IN APULIA A. A. de Souza, Centro de Citricultura Sylvio Moreira, IAC Cordeirópolis, São Paulo, Brazil Chairman: Prof. A. H. Purcell 12:30-13:00 THE OLIVE QUICK DECLINE SYNDROME 13:00-13:20 IDENTIFICATION AND CHARACTERIZATION OF FUNGAL SPECIES ASSOCIATED WITH THE QUICK DECLINE SYNDROME OF OLIVE 13:20-13:40 G. P. Martelli, Professor Emeritus, Dept. of Soil, Plant and Food Science, University of Bari Aldo Moro, Italy F. Nigro, I. Antelmi, A. Ippolito, Dept. of Soil, Plant and Food Science, University of Bari Aldo Moro, Italy OCCURENCE OF XYLELLA FASTIDIOSA IN APULIA D. Boscia, CNR Institute for Sustainable Plant Protection, UOS Bari, Italy 13:40-15:00 LUNCH 15:00-15:15 SURVEY OF THE AUCHENORRHYNCHA IN THE SALENTO PENINSULA IN SEARCH OF PUTATIVE VECTORS OF XYLELLA FASTIDIOSA STRAIN CODIRO 15:15-15:30 SESSION III 15:30-15:50 D. Cornara, G. Loconsole, D. Boscia, A. De Stradis, R. Y. Yokomi, D. Bosco, F. Porcelli, M. Saponari OBSERVATIONS ON THE BIOLOGY AND ETHOLOGY OF APHROPHORIDAE: PHILAENUS SPUMARIUS (L.) IN THE SALENTO PENINSULA D. Cornara, F. Porcelli, Dept. of Soil, Plant and Food Science, University of Bari, Italy DETECTION AND CHARACTERIZATION OF XYELLA FASTIDIOSA Chairman: Prof. J. D. Janse AN INNOVATIVE MONITORING MODEL FOR XYLELLA FASTIDIOSA IN APULIA REGION, ITALY A. M. D‟ Onghia, F. Santoro, T. Yaseen, K. Djelouah, A. Guario, A. Percoco, T. Caroppo, F. Valentini 12 15:50-16:10 MODELLING THE SPREAD OF XYLELLA FASTIDIOSA IN APULIA (SOUTHERN ITALY) 16:10-16:30 INTERLABORATORY VALIDATION OF MOLECULAR AND SEROLOGICAL DIAGNOSIS OF XYLELLA FASTIDIOSA STRAIN CODIRO IN SUSCEPTIBLE HOSTS S. M. White, J. M. Bullock, D. A. P. Hooftman, D. S. Chapman G. Loconsole, O. Potere, T. Elbeaino, D. Frasheri, S. Frisullo, F. Palmisano, D. Boscia, M. Saponari 16:30-16:50 COFFEE BREAK 16:50-17:10 ISOLATION, GENETICS AND PRELIMINARY DATA ON THE PATHOGENICITY OF THE XYLELLA FASTIDIOSA CODIRO STRAIN 17:10-17:30 17:30-17:50 M. Saponari, G. Loconsole, R. Almeida, H. Della Coletta Filho, G. P. Martelli, D. Boscia DRAFT GENOME SEQUENCE OF XYLELLA FASTIDIOSA STRAIN CODIRO A. Giampetruzzi, M. Chiumenti, M. Saponari, G. Donvito, A. Italiano, G. Loconsole, C. Cariddi, G. P. Martelli, P. Saldarelli IDENTIFICATION AND CHARACTERIZATION OF XYLELLA FASTIDIOSA ISOLATED FROM COFFEE PLANTS IN FRANCE B. Legendre, S. Mississipi, V. Olivier, E. Morel, D. Crouzillat, K. Durand, P. Portier, F. Poliakoff, M. A. Jacques 17:50-18:30 20:00 POSTER VIEWING SESSION SOCIAL DINNER AT MASSERIA LI SAULI, GALLIPOLI (LECCE) 13 Wednesday, 22nd October SESSION IV 08:30-08:45 08:45-09:15 CURRENT ACTIONS FOR XYELLA FASTIDIOSA CONTROL Chairmen: B. C. Faraglia; G. Papa Pagliardini OPENING REMARKS V. N. Savino, Dept. of Soil, Plant and Food Science, University of Bari Aldo Moro, Italy PHYTOSANITARY REGULATION AGAINST XYLELLA FASTIDIOSA FROM A EUROPEAN UNION PERSPECTIVE G. Cardon, Directorate General for Health and Consumers, European Commission 09:15-09:45 MEASURES TAKEN BY THE ITALIAN PLANT PROTECTION SERVICE 09:45-10:15 B. C. Faraglia, Ministry of Agricultural, Food and Forestry Policies, Italy A. Guario, Rural Development Policy Area, Regional Phytosanitary Service, Apulia, Italy Risk assessment of Xylella fastidiosa for the EU G. Stancanelli, R. Almeida, D. Bosco, C. Bragard, D. Caffier, J. C. Gregoire, S. Parnell, G. Strona, O. Mosbach-Schulz, E. Czwienczek, G. Hollo 10:15-10:45 COFFEE BREAK 10:45-11:15 EPPO SUPPORTING THE EVOLUTION OF PHYTOSANITARY SYSTEMS IN EPPO MEMBER COUNTRIES F. Petter, F. Grousset & M. Suffert, EPPO European and Mediterranean Plant Protection Organization 11:15-12:15 OPEN DISCUSSION 12:15-12:30 CONCLUDING REMARKS M. Barba, Coordinator of the Advisory Scientific Committee for Xylella fastidiosa 12:30-13:30 LUNCH 14:00-17:30 FIELD TRIP 19:00-21.00 OLIVE PICKING TRIP: NIGHT HARVESTING OF OLIVES 21.00 BUFFET AT ANTICA MASSERIA DEL FANO, SALVE (LECCE) 14 PROGRAMME SCHEDULE WORKSHOP SESSIONS Thursday, 23th October 07:3009:30-10:00 DEPARTURE FOR LOCOROTONDO (BARI) FOR THE TRAINING WORKSHOPS COFFEE BREAK TRAINING WORKSHOP A CURRENT TOOLS FOR THE DETECTION OF XYLELLA FASTIDIOSA IN HOST PLANT AND VECTORS 10:00-11:00 PRESENTATION OF THE COURSE AND OVERVIEW ON THE DIAGNOSTIC TOOLS 11:00-13:00 LABORATORY WORK: ELISA, DNA ISOLATION, PCR AND QPCR 13:00-14:30 LUNCH 14:30-18:00 LABORATORY WORK: ELISA, DNA ISOLATION, PCR AND QPCR (CONTINUATION) M. Saponari, G. Loconsole, O. Potere, F. Palmisano, F. Valentini, H. D. Coletta Filho, D. Boscia TRAINING WORKSHOP B CHARACTERISTICS AND IDENTIFICATION OF XYLEM-SAP FEEDERS 10:00-11:00 PRESENTATION OF THE COURSE AND OVERVIEW ON TAXONOMY AND BIOLOGY OF INSECT VECTORS OF XILELLA FASTIDIOSA A. H. Purcell, D. Bosco, F. Porcelli, D. Cornara, L. Picciau 11:00-13:00 LABORATORY WORK 13:00-14:30 LUNCH 14:30-18:00 LABORATORY WORK AND FIELD SESSION 19:30-20:30 VISIT TO A LOCAL WINERY 20:30 DINNER AT A LOCAL RESTAURANT 16 Friday, 24th October TRAINING WORKSHOPS A-B 08:30-16:30 LABORATORY WORK (CONTINUATION) 12:45-14:00 LUNCH 16:00-16.30 DISCUSSION AND CONCLUDING REMARKS 17
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