生物情報科学研究教育支援 (RNAi) 寄附講座 (RNAi) Laboratory Supporting Undergraduate Program 2 for Bioinformatics and Systems Biology 平成 18 年度報告書 2007 年 4 月 1 ┘ḗ 㸦㸣ࡢࡋࡴ࡞ 㸧㸣ᐞㅦᗑࡡᴣさ 㸧㸣㸦 ᐞㅦᗑࡡྞ⛘ 㸧㸣㸧 ス⨠ᖳ᭮ 㸧㸣㸨 ス⨠㛣 㸧㸣㸩 ᐞ⩽ 㸧㸣㸪 ᐞ⩽ࡡᴣさ 㸧㸣㸫 ᐞ㔘㢘࡛ᐞ᪁Ἢ 㸧㸣㸬 ᢰᙔᩅဤཀྵࡦ⫃ྞ 㸨㸣ᖲᠺ㸦㸭ᖳᗐఌゝሒ࿈ 㸩㸣ᐞㅦᗑス❟ࡡ⤊⦃࡛ࡐࡡᚃࡡ⤊㐛 㸩㸣㸦 ᐞㅦᗑス❟ࡡ┘Ⓩ࡛⤊⦃ 㸩㸣㸧 ᐞㅦᗑࡡහᐖ 㸩㸣㸨 ᙽࡈࡿࡾᠺᯕ 㸪㸣ᐞㅦᗑࡡ㐘ႜ 㸪㸣㸦 㐘ႜጟဤఌࡡᵋᠺ 㸪㸣㸧 㐘ႜጟဤఌࡡ㛜ത࡛㆗ 㸪㸣㸨 㐘ႜጟဤఌ㆗㘋 㸫㸣ᐞㅦᗑᩅဤ 㸫㸣㸦 ᐞㅦᗑᩅဤ 㸫㸣㸧 ᐞㅦᗑᩅဤ㸝ຐᡥ┞ᙔ㸞ࡡ➠㸦ᅂපຽ 㸫㸣㸨 ᐞㅦᗑᩅဤ㸝ຐᡥ┞ᙔ㸞ࡡ➠㸧ᅂපຽ 㸬㸣⏍∸ሒ⛁Ꮥ⛁ࡡ⌟≟ 㸬㸣㸦 ◂✪࣬ᩅ⫩โᗐ 㸬㸣㸧 ெⓏ㈠⏐☔ಕ 㸬㸣㸨 ◂✪࣬ᩅ⫩⎌ሾᩒങ 㸭㸣⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩ࣈࣞࢡ࣑ࣚ㸝:5'8'㸞ࡡ⌟≟ 㸭㸣㸦 ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩ࣈࣞࢡ࣑ࣚࡡᴣさ 㸭㸣㸧 ᑍ㛓ᩅ⫩ࡡහᐖ 㸭㸣㸨 ᩅဤᵋᠺ 㸭㸣㸩 ᒓಞ࡛ಞッ࡞ࡗ࠷࡙ 㸭㸣㸪 ᖲᠺ㸦㸭ᖳᗐධᏕ⛁භ㏳⛁┘ 㸮㸣◂✪Ὡິ 㸮㸣㸦 ◂✪හᐖ 㸮㸣㸧 ◂✪ᠺᯕ 2 ࣭࣋ࢩ 3 4 6 7 9 11 15 17 24 㸦㸣ࡢࡋࡴ࡞ ᮶ாኬᏕ࡞࠽ࡄࡾ⏍∸ሒ⛁Ꮥࡡᩅ⫩◂✪࡞ኬࡀᙲࢅᯕ ࡒࡊ࡙ࡀࡒࠉᩝ㒂⛁Ꮥ┤⛁Ꮥᢇ⾙ᣲ⮾ㄢᩒ㈕࡞ࡻࡾ⌦Ꮥ㒂࡞࠽ ࡄࡾࠔ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩≁ืࣈࣞࢡ࣑ࣚࠕࡢࠉᖲᠺ㸦㸭ᖳ 㸨᭮ࢅᣚࡖ࡙⤂ࡊࡒࠊࡆࡡᠺຉࢅུࡄ࡙ࠉ᩺Ꮥ⛁ࠔ⏍∸ሒ ⛁Ꮥ⛁ࠕᖲᠺ㸦㸮ᖳ㸩᭮࡞ス❟ࡈࡿࡒࠉ㸨ᖳ⏍᩺Ꮥ⛁࡞ 㐅Ꮥࡌࡾࡡࡢᖲᠺ㸧㸦ᖳᗐ࡚࠵ࡾࠊ୦⩽㛣ࡡ㛣Ⓩ࢟ࣔࢴࣈ ᇔࡴࡼࡿࡄࡿࡣࠉ ࠔ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩≁ืࣈࣞࢡ࣑ࣚࠕ࡚ ⠇࠷࡙ࡀࡒ᮶ாኬᏕ࡞࠽ࡄࡾ⏍∸ሒ⛁Ꮥࡡᩅ⫩ࡵ◂✪ࡵࠉࡐ ࡡࣝ࣊ࣜኬࡀࡂⴘࡔ㎲ࢆ࡚ࡊࡱ࠹ࠊࡆࡿࢅ㜭ࡃࡒࡴ࡞ࠉ⏍∸ ሒ⛁ᏕᏕ㒂ᩅ⫩≁ืࣈࣞࢡ࣑࡛ࣚྜྷ➴ࡡࣈࣞࢡ࣑࡚ࣚ࠵ࡾ ࠔ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩ࣈࣞࢡ࣑ࣚࠕᏕහ᥈⨠࡛ࡊ࡙ᖲᠺ㸦 㸭ᖳ㸩᭮࠾ࡼ㛜ጙࡈࡿࡒࠊᮇᨥᥴᐞㅦᗑࡡ᭩ኬࡡ┘Ⓩࡢࠉ᩺ ࣈࣞࢡ࣑ࣚ࠽ࡻࡦ᩺Ꮥ⛁㐘ႜࡡ᭩ኬࡡ࣍ࢹࣜࢾࢴࢠ࡚࠵ࡾຐᡥ ┞ᙔࢅ୯ᚨ࡛ࡊࡒᩅဤ㊂ࢅࠉ࡚ࡀࡾࡓࡄ࠽࠹࡛࠷࠹ࡵࡡ࡚ ࠵ࡾࠊᚉࡖ࡙ࠉᮇᨥᥴㅦᗑ࡚ࡢࠉ࠵ࡾ≁ᏽࡡࢷ࣭࣏ࡡよỬࡓࡄ ࢅ┘ᣞࡌࠉኣࡂࡡᚉᮮᆵࡡᐞㅦᗑࡡሔྙ࡛ࡢ␏ࡽࠉࡳࡊࢀ ኬᏕ࡞࠽ࡄࡾᩅ⫩ࡡὮິ࡞✒ᴗⓏ࡞ᑊᚺࡌࡾࡆ࡛࡞ྡྷࡄࡼࡿ ࡾࡵࡡ࡚࠵ࡾࠊ 3 㸧㸣ᐞㅦᗑࡡᴣさ 㸧㸣㸦 ᐞㅦᗑࡡྞ⛘ 㸝ᩝ㸞⏍∸ሒ⛁Ꮥ◂✪ᩅ⫩ᨥᥴ73&Lᐞ㜻ㅦᗑ 㸝ⱝᩝ㸞(RNAi) Laboratory Supporting Undergraduate Program 2 for Bioinformatics and Systems Biology 㸧㸣㸧 ス⨠ᖳ᭮ ᖲᠺ㸦㸭ᖳ㸩᭮㸦 㸧㸣㸨 ス⨠㛣㸝ᏽ㸞 ᖲᠺ㸦㸭ᖳ㸩᭮㸦̾ᖲᠺ㸧㸨ᖳ㸨᭮㸨㸦㸝㸪ᖳ㛣㸞 㸧㸣㸩 ᐞ⩽ ᰬᘟఌ♣ 73&Lཱི⥶ᙲఌ㛏ࠉ᮶ாᕝᴏኬᏕኬᏕ㝌≁௴ᩅ ྞཱིᖶ ᰬᘟఌ♣ࣜࣆࢩ࢘ࣤ♣࠽ࡻࡦᰬᘟఌ♣ 73&L㸝ᖲᠺ㸦㸮ᖳᗐ௧㜾㸞 㸧㸣㸪 ᖲᠺ ᖳᗐᐞ⩽㸝73&L ♣㸞ࡡᴣさ 㸝㸦㸞ス❟ᖳ᭮㸯㸧㸥㸥㸩ᖳ㸧᭮㸧㸬 㸝㸧㸞㈠ᮇ㔘㸯㸦㸡㸥㸮㸬Ⓤළ㸝 ᖳ㸮᭮ᮆ⌟ᅹ㸞 㸝㸨㸞୕ࡅ㸯㸨Ⓤළ㸝 ᖳᗐ୯㛣㸞 㸝㸩㸞ᚉᴏဤᩐ㸯㸧㸬ྞ㸝 ᖳ㸮᭮⌟ᅹ㸯ᙲဤ࣬Ὤ㐭♣ဤ࣬ዉ⣑♣ဤࢅྱࡳ㸞 㸝㸪㸞ᴏࡡᴣ␆ 73&L㸝73&LQWHUIHUHQHH㸞ᢇ⾙ࢅᇱ┑࡛ࡊࡒ◂✪ᨥᥴࢦ࣭ࣄࢪᴏ࠽ࡻࡦᰶ㓗 ⷾᴏࠊ ◂✪ᨥᥴࢦ࣭ࣄࢪᴏࡢ ᖳ ᭮ࡻࡽ⿿ဗ㈅ࢅ㛜ጙࡊࠉᅗ හ࢜ࢸ࣐࡞ࡢスゝࢨࢪࢷ࣑ࢅ↋ർ࡚ࠉ◂✪ᮞᩩ VL73&㸝VKRUWLQWHUIHULQJ 73&㸞ࢅᕰ౮ࡡᩐฦࡡ㸦ࡡ౮࡚ᥞ౩ࠊ ᰶ㓗ⷾᴏࡢⷾࢰ࣭ࢣࢴࢹࡡ⤘㎲ ࡲ࡞Ἰງࡊ࡙࠽ࡽࠉ ᖳ௧㜾࡞ᮇⓏ࡞❟୕ࡅᏽࠊ 㸧㸣㸫 ᐞ㔘㢘࡛ᐞ᪁Ἢ ⥪㢘 ࠉࠉ ළ ⌟㔘࡚⣙㸝ฦ㸞 ᐞ㜻 4 㸧㸥㸥㸪ᖳ㸦㸦᭮ ࠉࠉ ළ 㸝㸧㸥㸥㸫ᖳฦࢅ๑ࡊ࡚ᐁ᪃㸞 㸧㸥㸥㸬ᖳ㸧᭮ ࠉࠉ ළ 㸧㸥㸥㸭ᖳ㸧᭮ ࠉࠉ ළ 㸧㸥㸥㸮ᖳ㸧᭮ ࠉࠉ ළ 㸧㸥㸦㸥ᖳ㸧᭮ ࠉࠉ ළ 㸧㸣㸬 ᢰᙔᩅဤཀྵࡦ⫃ྞ ⏍∸ሒ⛁Ꮥ⛁㸝௫⛘㸰ᖲᠺ 19 ᖳᗐス⨠ᏽ㸞ᢰᙔࡡ⌦Ꮥ⣌◂✪⛁ᩅ㸦ྞཀྵ ࡦྜྷຐᩅ㸦ྞࢅࠉె௴ᩅཀྵࡦె௴ຐᩅ࡛ࡌࡾࠊࡈࡼ࡞ࠉපຽ࡞ࡻࡖ࡙㐽 ฝࡈࡿࡒᐞㅦᗑᩅဤ㸝ຐᡥ┞ᙔ㸞㸧ྞ࠾ࡼᵋᠺࡈࡿࡾࠊ 5 㸨㸣ᖲᠺ㸦㸭ᖳᗐఌゝሒ࿈ ථ ᦤさ ུථ㢘 㸝ᰬ㸞73&L ♣ࡻࡽᐞ ᦤ⏕ ㈜㔘㸝ᐞㅦᗑᩅဤ㸞 Ὤ㐭ᢇ⾙ဤ㈜㔘 ㅨ㔘㸝ຸဤ㸞 ᨥฝ ᐁ㥺モⷾ➴ ᪉㈕ ᨥฝ㢘 㞹Ẵ࣬࢝ࢪᩩ㔘 ᨥฝゝ ṟ㧏 6 㸩㸣ᐞㅦᗑス❟ࡡ⤊⦃࡛ࡐࡡᚃࡡ⤊㐛 㸩㸣㸦 ᐞㅦᗑス❟ࡡ┘Ⓩ࡛⤊⦃ ᮶ாኬᏕ࡞࠽ࡄࡾ⏍∸ሒ⛁Ꮥࡡᩅ⫩◂✪࡞ኬࡀᙲࢅᯕࡒࡊ࡙ࡀࡒࠉᩝ㒂 ⛁Ꮥ┤⛁Ꮥᢇ⾙ᣲ⮾ㄢᩒ㈕࡞ࡻࡾ⌦Ꮥ㒂࡚ࡡ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩≁ืࣈࣞࢡ ࣑ࣚ㸝:5'8'㸞ࡢࠉᖲᠺ㸦㸭ᖳ㸨᭮ࢅᣚࡖ࡙⤂ࡊࡒࠊࡆࡿ࡞ࢂࡾ᩺Ꮥ⛁ࡡス ❟ࡢᖲᠺ㸦㸮ᖳᗐ࡞ᏽࡈࡿ࡙࠷ࡾࠉ㸨ᖳ⏍㐅Ꮥࡊ࡙ࡂࡾࡡࡢᖲᠺ㸧㸦ᖳ ᗐ࡛ࡾࠊᚉࡖ࡙ࠉ୦⩽㛣࡞ࡢ㸨ᖳ㛣ࡡ㛣Ⓩ࢟ࣔࢴࣈᏋᅹࡊࠉࡆࡡࡱࡱ࡚ ࡢࠉ᮶ாኬᏕ࡞࠽࠷࡙༎ฦᐁ⦴ࢅ✒ࡲ୕ࡅ࡙ࡀࡒ⏍∸ሒ⛁Ꮥࡡᩅ⫩࣬◂✪ ࡵ୯᩷ࡎࡉࡾࢅᚋࡍࠉࡆࡿࡱ࡚ࡡᩅ⫩࣬◂✪ຝᯕኬࡀࡂࢂࡿ࡙ࡊࡱ࠹ࠊ ࡆࡿࢅ㜭ࡃࡒࡴ࡞ࡢࠉ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩≁ืࣈࣞࢡ࣑࡛ࣚྜྷ➴ࡡࣈࣞࢡࣚ ࣑:5'8'ࢅ᩺Ꮥ⛁ス❟ࡱ࡚ࡡ࢟ࣔࢴࣈ㛣࡞⤽⤾ࡈࡎࡾᚪさ࠵ࡾࠊᮇᨥᥴ ᐞㅦᗑࡡ᭩ኬࡡ┘Ⓩࡢࠉࡐࡡ㝷ࡡ᭩ኬࡡ࣍ࢹࣜࢾࢴࢠ࡚࠵ࡾຐᡥ┞ᙔࢅ୯ᚨ ࡛ࡊࡒᩅဤ㊂ࢅࠉ࡚ࡀࡾࡓࡄ࠽࠹࡛࠷࠹ࡵࡡ࡚࠵ࡾࠊࡱࡒࠉᏕ⛁ス❟࡞ ࡢࠉࡐࡡమโඖᐁࡌࡾࡱ࡚ࡡิ㛣ࡡᏕ⛁࡞࠽ࡄࡾᩅ⫩㸝࡛ࡊ࡙ᐁ㥺࣬ ᐁ⩞㸞ࢅࡵᨥᥴࡌࡾࠊᚉࡖ࡙ࠉᮇᨥᥴㅦᗑࡡ┘Ⓩࡢࠉ࠵ࡾ≁ᏽࡡࢷ࣭࣏ࡡよỬ ࡓࡄࢅ┘ᣞࡌࠉኣࡂࡡᚉᮮᆵࡡᐞㅦᗑࡡሔྙ࡛ࡢ␏ࡽࠉ᛬⃥࡞㐅Ṅࡊࡗࡗ ࠵ࡾኬぞᶅ㐿ఎᏄよᯊ࣬᧧షᢇ⾙ࢅ୯ᚨ࡛ࡊࡒ⏍∸ሒ⛁Ꮥᩅ⫩ࡡὮິ࡞✒ ᴗⓏ࡞ᑊᚺࡌࡾࡆ࡛࡞ྡྷࡄࡼࡿ࡙࠷ࡾࠊࡱࡒࠉࡆࡡࡻ࠹⌦⏜࠾ࡼࠉ᩺Ꮥ⛁ᢰ ᙔ⌦Ꮥ⣌◂✪⛁ᩅဤ࡛ᐂဤᩅဤୌమ࡞ࡖࡒ༝ງమโࡡᵋ⠇ᚪ㡪࡛ࡾࠊ 㸩㸣㸧 ᐞㅦᗑࡡහᐖ ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩≁ืࣈࣞࢡ࣑࡛ࣚྜྷ➴ࡡහᐖࡡࣈࣞࢡ࣑ࣚ:5'8' ࢅᖲᠺ㸦㸭ᖳ㸩᭮௧㜾᩺Ꮥ⛁ᠺ❟ࡱ࡚ᐁ᪃ࡌࡾࠊᐞㅦᗑᐂဤᩅဤࡢࠉኚఆ ᬜ୯࡞⾔࠹⏍⛁Ꮥ⣌Ꮥ⏍࡞ᑊࡌࡾࢤࣤࣅ࣭ࣖࢰ⛁Ꮥᐁ⩞࡛∸⌦࣬ሒ⣌Ꮥ⏍ ࡞ᑊࡌࡾ⏍⛁Ꮥᐁ㥺ࢅᣞᑙࡌࡾ࡛ྜྷ࡞ࠉె௴ᩅࠉຐᩅ࡛⏍∸ሒ⛁Ꮥ ࡞㛭ࡌࡾᖕᗀ࠷භྜྷ◂✪ࢅ⾔࠹ࠊᏕ⛁ࡡᠺ❟ᚃࡢࠉ୕オභྜྷ◂✪ࡓࡄ࡚ࡂᏕ ⛁ᡜᒌࡡᏕ⏍ࡡᐁ㥺ࡡᣞᑙࡡୌ⩴ࡵᢰ࠹ࠊ 㸩㸣㸨 ᙽࡈࡿࡾᠺᯕ ᩝ㒂⛁Ꮥ┤⛁Ꮥᢇ⾙ᣲ⮾ㄢᩒ㈕࡞ࡻࡾ⌦Ꮥ㒂࡚ࡡ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩≁ืࣈ 7 ࣞࢡ࣑ࣚ࡞ࡻࡾ⏍∸ሒ⛁Ꮥࡡᩅ⫩ཀྵࡦ◂✪࣎ࢷࣤࢨࣔࣜࡡୖࢅ㜭ࡁࠉᖳ㛣 㸨㸥̾㸩㸥ྞࡡ⌦Ꮥ㒂Ꮥ⏍࡞ࠉ⏍∸ሒ⛁Ꮥࡡᐁ⩞࣬ᐁ㥺ࢅ⾔࠹ࢲࣔࣤࢪࢅ⤽⤾ ࡊ࡙࠻ࡾࠊࡱࡒࠉຐᡥ࠷࠷ె௴ᩅࠉຐᩅࢅ◂✪㟻࡚ࢦ࣭࣎ࢹࡌࡾࡆ ࡛࡚ࠉ᮶ாኬᏕ࡞࠽ࡄࡾ⏍∸ሒ⛁Ꮥࡡ◂✪ࢅᙁງ࡞᥆㐅ࡌࡾࠊᮇᐞ㜻ㅦᗑス ⨠࡞ࡻࡽࠉᮇᏕᅗහ࣬ᅗ㝷Ⓩ࡞ࡵౚࢅぜ࠷⏍∸ሒ⛁Ꮥࡡ㥉Ⓩᩅ⫩࣬ ◂✪ᶭ㛭࡛ࡊ࡙ࡡᇱ┑ࢅᵋ⠇ࡌࡾࡆ࡛ྊ⬗࡞ࡾࠊ 8 㸪㸣ᐞㅦᗑࡡ㐘ႜ ᙔᐞㅦᗑࡡ㐘ႜ᪁㔢ࡢࠉᐞㅦᗑ㐘ႜጟဤఌ࡚ࡡណᛦỬᏽ࡞ࡻࡽỬᏽࡈࡿࡾࠊ 㸪㸣㸦 㐘ႜጟဤఌࡡᵋᠺ 㐘ႜጟဤఌࡢ௧ୖࡡጟဤ㸪ྞ࡛࢛ࣇࢧ࣭ࣁ࣭㸦ྞ࡚ᵋᠺࡈࡿࡾࠊ こ㒋 ⷽ ᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁ᩅ ᒜᮇ ḿᖶ ᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁ᩅ ᶋᒜ Ⱪ ᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁ᩅ 㯦⏛ ┷ஒ ᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁ᩅ ⛤ ⨶Ꮔ ᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁ຐᩅ 㧏ᮄ ฺ㸝࢛ࣇࢧ࣭ࣁ࣭㸞᮶ாኬᏕ᩺㡷ᇡᠺ⛁Ꮥ◂✪⛁ᩅ 㸪㸣㸧 㐘ႜጟဤఌࡡ㛜ത࡛㆗ 㸯ᖲᠺ ᖳ ᭮ ༔๑ ࡻࡽ ሔᡜ㸯⌦Ꮥ㒂 ྒ㤃 ྒᐄ ㆗㸯ᐞㅦᗑࡡ㐘ႜ࡞ࡗ࠷࡙ࠊ 㐘ႜጟဤఌࡡ㛜തࠊ ሒ࿈᭡➴ࡡᥞฝࠊ ࡐࡡ 㸪㸣㸨 㐘ႜጟဤఌ㆗㘋 ⏍∸ሒ⛁Ꮥ◂✪ᩅ⫩ᨥᥴ73&Lᐞ㜻 ㅦᗑ㐘ႜጟဤఌ 㸯ᖲᠺ ᖳ ᭮ ༔๑ ࡻࡽ ሔᡜ㸯⌦Ꮥ㒂 ྒ㤃 ྒᐄ ฝᖆ⩽㸯こ㒋ࠉᒜᮇࠉ㯦⏛ࠉ⛤ࠉ㧏ᮄ㸝࢛ࣇࢧ࣭ࣁ࣭㸞 㸦㸞 ᐞㅦᗑࡡ㐘ႜ࡞ࡗ࠷࡙ࠊ ⤊㈕ࡡ㏭࡞ࡗ࠷࡙ ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩ࣈࣞࢡ࣑ࣚ࡞ᑊࡌࡾኬᏕഁ࠾ࡼࡡᨥᥴ㈕⏕ࠉࡡ⛤ᗐ ࡗࡂࡡ࠾࡞ࡻࡽࠉᐞㅦᗑࡡ⤊㈕ࡡ㏭ኬࡀࡂ␏ࡾྊ⬗ᛮ㧏࠷ࡒࡴࠉレ 9 ⣵࡞ࡗ࠷࡙ࡢࠉኬᏕ࠾ࡼຐࡈࡿࡾ㔘㢘Ửᏽࡊ࡙࠾ࡼࠉᑊᚺࡎࡉࡾࢅᚋ࠷ࠊ ᐞ㜻ㅦᗑࡡ⤊㈕ࡡྊ⬗㏭ ⏕ྊ⬗㡧┘ࡢࠉ௧ୖ࡚࠵ࡾࠊ 㸝㸦㸞ெ௲㈕㸯ᐞㅦᗑᩅဤࠉຸဤ࣭ࡌ࡚࡞ࠉᐞㅦᗑᩅဤ㸝ຐᡥ┞ᙔ㸞࡛ Ὤ㐭ᢇ⾙ဤࢅ㞘⏕ࡌࡾᏽ࡛ࡖ࡙࠷ࡾࠊຸဤ࡞ࡗ࠷࡙ࡢࠉᙔ㟻ࡢࠉこ㒋 ⏍ࠉ㧏ᮄ⏍ࡡጟ௴⤊⌦㔘࡚㞘⏕ࡌࡾࠊࡐࡿ௧㜾ࡢࠉጟ௴⤊⌦㔘ࢅ㞗ࡴࡾຑງ ࢅࡊࠉ࡚ࡀࡾ㝀ࡽࠉᐞㅦᗑࡡ⤊㈕ࢅ⏕ࡊ࠷᪁㔢࡛ࡌࡾࠊᇱᮇⓏ࡞ࡢࠉ ኬᏕ࠾ࡼຐࡈࡿࡾ⤊㈕ࢅඖ࡙ࡾࠊ 㸝㸧㸞ᾐ⪎ဗ㸝◂✪㈕ࢅྱࡳ㸞࣭࡛ࡊ࡙ࠉ◂✪⏕ࡡᾐ⪎ဗ࡛ࡊ࡙⏕ࡌࡾࠊ 㸝㸨㸞᪉㈕࣭ᐞㅦᗑᩅဤ࠽ࡻࡦ௴ᩅဤࡡ᪉㈕࡛ࡊ࡙⏕࡚ࡀࡾࠊ 㸝㸩㸞ක⇍㈕࣭ᇱᮇⓏ࡞ࡢࠉኬᏕ࠾ࡼຐࡈࡿࡾ⤊㈕࡚ᨥᡮ࠹ࠊ 㸝㸪㸞ങဗ 㸝㸫㸞ㅨ㔘 㸝㸬㸞ࡐࡡ ຐᡥ┞ᙔࡡ㞘⏕ ຐᡥ┞ᙔࡡ㞘⏕ࡢࠉපຽ࡚Ửࡴࡾࡆ࡛࡛ࡌࡾࠊ 㸧㸞㐘ႜጟဤఌࡡ㛜തࠊ ᐞㅦᗑ㐘ႜጟဤఌࡢࠉᖳ㸦ᅂ㛜തࡌࡾࡆ࡛࡛ࡌࡾ㸝ḗᅂࡢࠉᮮᖳ㸨᭮㡥㸞ࠊ༝ ㆗ࡊࡄࡿࡣࡼ࠷᱄௲࡞ࡗ࠷࡙ࡢࠉᇱᮇⓏ࡞ࡢ࣒࣭࡚ࣜ⾔࠹ࠊ 㸨㸞ሒ࿈᭡➴ࡡᥞฝࠊ 㐘ႜጟဤఌ㆗㘋ࠉఌゝሒ࿈᭡ࠉᠺᯕሒ࿈᭡ࡢᖳᗐᮆ࡞షᠺࡌࡾࠊ ⏍ຸ࠽ࡻࡦ 73&L ♣ᥞฝࡌࡾᏽ࡛ࡌࡾࠊ ࡡࡻ࠹ሒ࿈᭡ࢅషᠺࡊࠉࡆᥞฝࡌࡀ࠾࡞ࡗ࠷࡙ࡡレ⣵ࡢࠉ◂✪༝ງ ౿࡛┞ㄧࡌࡾࠊ 㸩㸞ࡐࡡ ⌟ᅹࡡᐞㅦᗑࡡᐞ㜻ࡢこ㒋⏍࡛ࡖ࡙࠷ࡾࠉᮮᖳᗐ௧㜾ࠉ㯦⏛⏍ࡱ ࡒࡢ⏍ᑍᨯ㛏ࠉࡡࡻ࠹࡞ࡌࡾࡡ࠾ࢅ᳠ゞࡌࡾࠊ 10 㸫㸣ᐞㅦᗑᩅဤ 㸫㸣㸦 ᐞㅦᗑᩅဤ ᐞㅦᗑ௴ᩅဤ ᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁⏍∸Ꮥᑍᨯᩅ࣬㯦⏛┷ஒࠉྜྷຐᩅ࣬⛤⨶ Ꮔຸࡌࡾࡆ࡛ࠉᏕ⾙㐘ႜጟဤఌࡼࡦ࡞⌦Ꮥ⣌◂✪⛁ᩅఌ࡚ᢆヾࡈࡿ ࡒ㸝ᖲᠺ㸦㸭ᖳ㸪᭮㸦㸞ࠊ ᐞㅦᗑᩅဤ㸝ຐᡥ┞ᙔ㸞 ➠㸦ᅂපຽ㸝㸫㸣㸧㸞ࡡ⤎ᯕࠉಕ⏛ᾀ⾔ᐞㅦᗑᩅဤ㸝ຐᡥ┞ᙔ㸞࡛ࡊ࡙ ᖲᠺ㸦㸭ᖳ㸩᭮㸦࡞╌௴ࡊࡒࠊ ➠㸧ᅂපຽ㸝㸫㸣㸨㸞ࡡ⤎ᯕࠉහ⸠㞕ᶖᐞㅦᗑᩅဤ㸝ຐᡥ┞ᙔ㸞࡛ࡊ࡙ᖲ ᠺ㸦㸮ᖳ㸩᭮㸦࡞╌௴ࡊࡒࠊ 㸫㸣㸧 ᐞㅦᗑᩅဤ㸝ຐᡥ┞ᙔ㸞ࡡ➠ ᅂපຽ පຽさ㡧㸯 පຽெဤ㸯ᐞ㜻ㅦᗑᩅဤ㸝ຐᡥ┞ᙔ㸞㸦ྞ ᑍ㛓ฦ㔕㸯ࢨࢪࢷ࣑⏍∸Ꮥ ᕵ᭻ࡌࡾெᮞ㸯᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁⏍∸Ꮥᑍᨯ࡞ᖲᠺ ᖳ ᭮ ࡻࡽ ᖳ㛣ࡡᏽ࡚ス⨠ࡈࡿࡾ⏍∸ሒ⛁Ꮥ◂✪ᩅ⫩ᨥᥴᐞ㜻ㅦᗑ㸝73&L ᐞ㜻 ㅦᗑ㸞࡞ᡜᒌࡊࠉ⏍∸ሒ⛁Ꮥ⛁㸝௫⛘㸞ᩅဤࡡᣞᑙࡡࡵ࡛࡚◂✪ࢅ⾔࠷ࠉᖲ ᠺ㸦㸭ᖳ㸩᭮ࡻࡽ᩺ࡊࡂጙࡱࡾᏽࡡ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩ࣈࣞࢡ࣑ࣚ࠽ࡻࡦࠉ ⏍∸ሒ⛁Ꮥ⛁㸝௫⛘㸞࡚ࡡࢨࢪࢷ࣑⏍∸Ꮥࡡᩅ⫩࣬◂✪࡞✒ᴗⓏ࡞ཤຊࡌࡾ ࡆ࡛࡚ࡀࡾெࠊࢨࢡࢻࣜఎ㐡ࡡฦ㔕࡚࣏ࢠ࣭ࣞࣝࢅ⏕࠷ࡒࢹࣚࣤࢪࢠࣛ ࣈࢹ࣭࣑⥑⨮Ⓩよᯊࢅྱࡳࢨࢪࢷ࣑⏍∸Ꮥࡡ㡷ᇡ࡚ࡡ◂✪ᐁ⦴࠵ࡾࡆ࡛ࠊ ᮇ⏕ᏽᩅဤࡢ⏍∸ሒ⛁Ꮥ⛁㸝௫⛘㸞⌟≟ࡡᩅဤᩐࡡ㛭౿࡚ࠉ࠾ࡽࡡ㛏 㛣ࡡᏕ⏍ᐁ⩞ᐁ㥺ᣞᑙࡵさịࡈࡿࡾࠊ ╌௴㸯ᖲᠺ ᖳ ᭮ 11 ຽ㞗ெဤ㸯ᐞ㜻ㅦᗑᩅဤ㸝ຐᡥ┞ᙔ㸞㸦ྞࠊ ௴㸯༟ᖳᗐዉ⣑ࠉ᭩ኬᘇ㛏ᐞ㜻ㅦᗑ⤽⤾㛣㸝 ᖳᏽ㸞 ᥞฝ᭡㢦㸯 L ᒓṌ᭡㸝ᏕṌཀྵࡦ⫃Ṍ㸞 LL ᴏ⦴┘㘋㸝ᰕㄖㄵᩝ࡛ࡐࡿ௧አࡡ⥪ㄕ➴࡞ฦࡄ࡙ୖࡈ࠷ࠊ≁࡞さㄵᩝ 㸝㸨⥽௧හ㸞࡞ࡢ㔔୷ࢅࡗࡄࡾࠊ㸞 LLL ㄵᩝืใ㸝㞹Ꮔࣆࣜ࠵ࡿࡣࣀ࣭ࢺࢤࣅ࣭ࡢᚪさࠊ㞹Ꮔࣆࣜ ࠷ሔྙࡢࢤࣅ࣭ࢅ 㒂ྜྷᑌ㸞 L[ ◂✪ᴏ⦴ࡡᴣさ㸝& ⏕⣤㸦ᯓ࡞さ⣑㸞 [ ᚃࡡ◂✪ゝ⏤ཀྵࡦᢢㇿ㸝& ⏕⣤㸦ᯓ࡞さ⣑㸞 [L ᡜぜࢅ࠹࠾࠻ࡾ᪁㸝㸦ྞ㸞ࡡẮྞཀྵࡦ㏻⤙ [LL ௧୕ L[L ࡡ㞹Ꮔࣆࣜ පຽ⥶ࡴวࡽ㸯ᖲᠺ ᖳ ᭮ 㸝᭮㸞ᚪ╌ ᭡㢦ᥞฝ㸯㒉౼␊ྒ ᮶ா㒌ᩝா༇ᮇ㒋㸬࣭㸨࣭㸦 ᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁ ⏍∸Ꮥᑍᨯຸᐄ 㸟ᑌ᭡࡞ࠔᐞ㜻ㅦᗑᩅဤᚺຽ᭡㢦ᅹ୯ࠕ࡛ᮊ᭡ࡊࠉ㒉౼ࡡሔྙࡢ᭡⏻࡚࠽㢢࠷ ࡊࡱࡌࠊ࠽ࠉᥞฝࡈࡿࡒ᭡㢦ࡢࠉཋ์࡛ࡊ࡙㏁༴࠷ࡒࡊࡱࡎࢆࠊ ၡ࠷ྙࢂࡎ㸯 ᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁ ⏍∸Ꮥᑍᨯ ᩅ ᶋᒜⱩ 9HO +F] *PFLO^RNR^FPF%GLRHKHPVXWRN^RFHMS පຽさ㡧ࡡ᥎㍍㸯 ᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁⏍∸Ꮥᑍᨯ࣭࣑࣭࣌࣋ࢩ /FSFQ7HVHFUHK(FUHHU.QIRUPFWLRQ3HW\FUN/7*(.3◂✪⩽ெᮞࢸ࣭ࢰ ࣭࣊ࢪ 㸫㸣㸨 ᐞㅦᗑᩅဤ㸝ຐᡥ┞ᙔ㸞ࡡ➠㸧ᅂපຽ පຽさ㡧㸯 12 පຽெဤ㸯ᐞ㜻ㅦᗑᩅဤ㸝ຐᡥ┞ᙔ㸞㸦ྞ ᑍ㛓ฦ㔕㸯⏍∸ሒ⛁Ꮥ ᕵ᭻ࡌࡾெᮞ㸯᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁⏍∸Ꮥᑍᨯ࡞ᖲᠺ㸦㸭ᖳ ᭮ ࡻࡽ ᖳ㛣ࡡᏽ࡚ス⨠ࡈࡿࡒ⏍∸ሒ⛁Ꮥ◂✪ᩅ⫩ᨥᥴᐞ㜻ㅦᗑ㸝73&L ᐞ㜻 ㅦᗑ㸞࡞ᡜᒌࡊࠉ⏍∸ሒ⛁Ꮥ⛁㸝௫⛘㸞ᩅဤࡡᣞᑙࡡࡵ࡛࡚◂✪ࢅ⾔࠷ࠉᖲ ᠺ㸦㸭ᖳ㸩᭮ࡻࡽጙࡱࡖ࡙࠷ࡾ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩ࣈࣞࢡ࣑ࣚ࠽ࡻࡦࠉᖲᠺ 㸦㸮ᖳ㸩᭮ス❟ᏽࡡ⏍∸ሒ⛁Ꮥ⛁㸝௫⛘㸞࡚ࡡ⏍∸ሒ⛁Ꮥࡡᩅ⫩࣬◂✪ ࡞✒ᴗⓏ࡞ཤຊࡌࡾࡆ࡛࡚ࡀࡾெࠊࢣࢿ࣑ࢦ࢙ࣤࢪࡡฦ㔕࡞࠽ࡄࡾ⏍∸ ሒ⛁ᏕⓏよᯊࠉ≁࡞ 73& ᖱῦἪ㡷ᇡ࡚ࡡ◂✪ᐁ⦴࠵ࡾࡆ࡛᭻ࡱࡊ࠷ࠊᮇ ⏕ᏽᩅဤࡢ⏍∸ሒ⛁Ꮥ⛁㸝௫⛘㸞⌟≟ࡡᩅဤᩐࡡ㛭౿࡚ࠉ࠾ࡽࡡ㛏㛣 ࡡᏕ⏍ᐁ⩞ᐁ㥺ᣞᑙࡵさịࡈࡿࡾࠊ ╌௴㸯ᖲᠺ㸦㸮ᖳ㸩᭮ ຽ㞗ெဤ㸯ᐞ㜻ㅦᗑᩅဤ㸝ຐᡥ┞ᙔ㸞㸦ྞࠊ ௴㸯༟ᖳᗐዉ⣑ࠉ᭩ኬᘇ㛏ᐞ㜻ㅦᗑ⤽⤾㛣㸝 ᖳᏽ㸞 ᥞฝ᭡㢦㸯 L ᒓṌ᭡㸝ᏕṌཀྵࡦ⫃Ṍ㸞 LL ᴏ⦴┘㘋㸝ᰕㄖㄵᩝ࡛ࡐࡿ௧አࡡ⥪ㄕ➴࡞ฦࡄ࡙ୖࡈ࠷ࠊ≁࡞さㄵᩝ 㸝㸨⥽௧හ㸞࡞ࡢ㔔୷ࢅࡗࡄࡾࠊ㸞 LLL ㄵᩝืใ㸝㞹Ꮔࣆࣜ࠵ࡿࡣࣀ࣭ࢺࢤࣅ࣭ࡢᚪさࠊ㞹Ꮔࣆࣜ ࠷ሔྙࡢࢤࣅ࣭ࢅ 㒂ྜྷᑌ㸞 L[ ◂✪ᴏ⦴ࡡᴣさ㸝& ⏕⣤㸦ᯓ࡞さ⣑㸞 [ ᚃࡡ◂✪ゝ⏤ཀྵࡦᢢㇿ㸝& ⏕⣤㸦ᯓ࡞さ⣑㸞 [L ᡜぜࢅ࠹࠾࠻ࡾ᪁㸝㸦ྞ㸞ࡡẮྞཀྵࡦ㏻⤙ [LL ௧୕ L[L ࡡ㞹Ꮔࣆࣜ පຽ⥶ࡴวࡽ㸯ᖲᠺ ᖳ ᭮ 㸝ᮄ㸞ᚪ╌ ᭡㢦ᥞฝ㸯㒉౼␊ྒ ᮶ா㒌ᩝா༇ᮇ㒋㸬࣭㸨࣭㸦 ᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁ ⏍∸Ꮥᑍᨯ࣬⏍∸ሒ⛁Ꮥຸᐄ 㸟ᑌ᭡࡞ࠔᐞ㜻ㅦᗑᩅဤᚺຽ᭡㢦ᅹ୯ࠕ࡛ᮊ᭡ࡊࠉ㒉౼ࡡሔྙࡢ᭡⏻࡚࠽㢢࠷ ࡊࡱࡌࠊ࠽ࠉᥞฝࡈࡿࡒ᭡㢦ࡢࠉཋ์࡛ࡊ࡙㏁༴࠷ࡒࡊࡱࡎࢆࠊ ၡ࠷ྙࢂࡎ㸯 ᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁ ⏍∸Ꮥᑍᨯ ᩅ 㯦⏛┷ஒ 13 9HO +F] *PFLOVNXURIF%GLVXWRN^RFHMS පຽさ㡧ࡡ᥎㍍㸯 ᮶ாኬᏕኬᏕ㝌⌦Ꮥ⣌◂✪⛁⏍∸Ꮥᑍᨯ࣭࣑࣭࣌࣋ࢩ /FSFQ7HVHFUHK(FUHHU.QIRUPFWLRQ3HW\FUN/7*(.3◂✪⩽ெᮞࢸ࣭ࢰ ࣭࣊ࢪ 14 㸬㸣⏍∸ሒ⛁Ꮥ⛁ࡡ⌟≟ 㸬㸣㸦 ◂✪࣬ᩅ⫩โᗐ ⏍⛁Ꮥ࡛ሒ⛁ᏕࡡᏕ㝷⼝ྙ㡷ᇡ࡞న⨠ࡌࡾ⏍∸ሒ⛁Ꮥࡢࠉ࣎ࢪࢹࢣࢿ࣑ ࡞ᑊᚺࡊࡒ᩺ࡊ࠷◂✪㡷ᇡ࡚࠵ࡽࠉᚃࡡ⏍⛁Ꮥࡡ◂✪࡞࠽࠷࡙ࠉ୯ᰶ Ⓩᙲࢅᯕࡒࡌ࡛ᙽࡈࡿ࡙࠷ࡾࠊࡱࡒࠉ⏐ᏻᏕ⏲࠾ࡼࡡ⏍∸ሒ⛁Ꮥᩅ⫩ ࢅུࡄࡒெᮞࡡ㞺さࡵ᛬㏷࡞ቌኬࡊ࡙࠷ࡾࠊ⏍∸ሒ⛁Ꮥ⛁ࡢᖲᠺ㸦㸮ᖳ㸩᭮ ࡞ス❟ࡈࡿࡾࠉ⏍⛁Ꮥ࡛ࢤࣤࣅ࣭ࣖࢰ⛁Ꮥࢅ⼝ྙࡈࡎࡒᏕ㒂ᩅ⫩࡛ࡊ࡙ࡢ ᠻᅗ࡚ิࡴ࡙࡛ࡾࠊᏕ⛁ス❟࡞❟ࡔࠉᖲᠺ ᖳᗐࡻࡽᩝ㒂⛁Ꮥ┤ࠉᣲ⮾ ㄢᩒ㈕ࠉ᩺⮾ฦ㔕ெᮞ㣬ᠺࠔ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩≁ืࣈࣞࢡ࣑ࣚࠕ࡞ࡻࡽࠉ ⌦Ꮥ㒂࡞࠽࠷࡙ࠉ⾔Ⓩ࡞⏍∸ሒ⛁ᏕࡡᏕ㒂ᩅ⫩ࢅ㛜ጙࡊࠉኣᩐࡡ༡ኃ◂✪ ဤࢅ㣬ᠺࡊࠉྙࢂࡎ࡙⏍∸ሒ⛁Ꮥࡡ◂✪ࡵ᥆㐅ࡊ࡙ࡀࡒࠊᏕ㒂ᩅ⫩࡞࠵ࡒࡖ ࡙ࡢࠉᣲ⮾ㄢᩒ㈕࡞ࡻࡾ≁௴ᩅဤࢅ୯ᚨ࡛ࡊ࡙ࠉ⌦Ꮥ⣌◂✪⛁࣬⏍∸Ꮥᑍᨯࠉ ሒ⌦ᕝᏕ⣌◂✪⛁࣬ࢤࣤࣅ࣭ࣖࢰ⛁Ꮥᑍᨯࠉ᩺㡷ᇡᠺ⛁Ꮥ◂✪⛁࣬ሒ⏍ ⛁Ꮥᑍᨯࠉ༈⛁Ꮥ◂✪ᡜࡡᩅဤࡡ༝ງࢅ௭ࡁࠉኬᏕ㝌⏍ࡵྱࡳ᮶ாኬᏕ ࡡධᏕ⏍࡛ୌ㒂♣ఌெࢅᑊ㇗࡛ࡊࡒᩅ⫩ࢅ⾔࠷ࠉ㸨ᖳ㛣࡚ྙゝ ྞ⛤ᗐࡡ࢜ࣛ ࣑࢞ࣖࣚಞ⩽ࢅ㍦ฝࡊࡒࠊࡱࡒࡆࡡࣈࣞࢡ࣑࡚ࣚࡢࠉ༡ኃ◂✪ဤࠉ≁௴ᩅဤࠉ ࠽ࡻࡦᏋᑍᨯࡡ༝ງᩅဤࠉᚉᮮࡡᯗࢅ㉰࠻࡙✒ᴗⓏ࡞භྜྷ◂✪ࡡ㍧ࢅᗀࡅࠉ ࢤࣤࣅ࣭ࣖࢰ⛁Ꮥ࡛ࡊ࡙ࡡࣁ࢛ࣤࣆ࢚࣏ࢷࢴࢠࢪ࡛ᐁ㥺⛁Ꮥ࡛ࡊ࡙ࡡ⏍ ⛁Ꮥ࡛ࡡࡻࡽᖕᗀ࠷⼝ྙ࡞ࡵຑງࡊࡒ⤎ᯕࠉᚉᮮࡡ◂✪㡷ᇡࡡᯗࢅ㉰࠻ࡒ◂ ✪ᐁ⦴ࢅࡓࡌࡆ࡛࡚ࡀࡒࠊࣈࣞࢡ࣑ࣚࡢᖲᠺ ᖳᗐ࡞⤂ࡊࡒࠉᖲᠺ ᖳᗐ࠾ࡼࡢᏕහ᥈⨠࡛ࡊ࡙ࠉ᩺ࣈࣞࢡ࣑ࣚࢅ❟ࡔ୕ࡅࠉ73&L ᐞㅦᗑࡡᨥᥴࡵ ུࡄࠉ⤽⤾Ⓩ࡞⏍∸ሒ⛁Ꮥᩅ⫩ࢅ⾔࠷ࠉᖲᠺ㸦㸭ᖳᗐ࡞ࡢ㸧㸦ྞࡡ࢜ࣛ࢞ࣖ ࣑ࣚಞ⩽ࢅ㍦ฝࡊࡒࠊࡆࡿࡱ࡚ࡡᐁ⦴ࢅᇱ┑࡞ࠉࡻࡽమ⣌Ⓩᩅ⫩ࣈࣞࢡࣚ ࣑ࢅ⥽ᠺࡊࠉ⌦Ꮥ⣌ධమ࠵ࡾ࠷ࡢ㒂ᑻ࡛ࡡ㏻ᦘࡵㅆࡽࡼࠉࡐࡿࡼࢅ㐲ว ࡞ཬ᫆ࡊࡒᩅ⫩మโࡡᵋ⠇࡞ཱིࡽ⤄ࡲࠉᏕ㒂Ꮥ⏍ࡡᩅ⫩ࣝ࣊ࣜࡡྡྷ୕ࡷࠉࡐࡿ ࡼࡡ⎌ሾࡷᏕ⏍ࡡ⤊ῥⓏᇱ┑ࡡᩒങࢅ⾔࠹ࠊ⏍∸ሒ⛁Ꮥ◂✪࣬ᩅ⫩࡞࠽࠷࡙ ୠ⏲Ⓩ࣭ࣛࢱ࣭ࢨࢴࣈࢅ࡛ࡿࡾெᮞࠉ࠽ࡻࡦ⏐ᴏ⏲ࢅᑙࡌࡾ⮤ᛮࡡ㧏࠷ ⱕᡥெᮞࢅ㣬ᠺ࣬㍦ฝࡌࡾࡆ࡛ࢅ┘ᣞࡊ࡙࠷ࡾࠊ 㸬㸣㸧 ெⓏ㈠⏐☔ಕ 15 ⌦Ꮥ㒂හ࡞࠽ࡄࡾ࣒ࢩ࣭࣏ࣔ࣬ࢻ࣭ᩅ⫩ࢅっ㔕࡞ථࡿࡒࠉୠ⏲᭩㧏Ề‵ࡡ◂ ✪࣬ᩅ⫩ࢅ᥆㐅ࡌࡾࡒࡴࡡᩅ⫃ဤࡡெဤ☔ಕ࡛ᩅဤᩅ⫩ࢅ⾔࠹ࠊࡱࡒࠉඁࡿࡒ ㈠㈻ࢅࡵࡗᏕ㒂Ꮥ⏍ࢅ☔ಕࡌࡾࡒࡴ࡞ࠉᗀሒὩິ㸝࣭࣑࣭࣌࣋ࢩࠉ࢝ࢱࣤࢪࠉ ࣏ࢪ࣒ࢸࢅࡊࡒሒⓆಘࠉ➴㸞ࠉ⤊ῥⓏᇱ┑ࡡ☔ಕࢅ✒ᴗⓏ࡞⾔࠷ࠉᏕ⏍ ࡞࡛ࡖ࡙㨡ງ࠵ࡾ⏍∸ሒ⛁Ꮥ⛁ࡡᵋ⠇࡞ຑࡴࡾࠊ 㸬㸣㸨 ◂✪࣬ᩅ⫩⎌ሾᩒങ ◂✪࣬ᩅ⫩ࣤࣆࣚࡡᩒങ࡞ࡗ࠷࡙ࡢࠉࡱࡍࠉᖲᠺ㸦㸮ᖳᗐථᏕࡡᏕ⏍ᖲᠺ 㸧㸦ᖳᗐ࡞㸨ᖳ⏍࡚㐅Ꮥࡊ࡙ࡂࡾࡱ࡚࡞ࠉᏕ㒂ᩅ⫩࡞ྊḖᩅ⫩⎌ሾᩒങࢅ ⾔࠹ࡆ࡛ᚪ⮫࡛ࡖ࡙࠷ࡾࠊ⏍∸ሒ⛁Ꮥ⛁࡚ࡢࠉ㏳ᖏࡡㅦ⩇࡞ຊ࠻࡙ࠉ⏍ ⛁Ꮥᐁ⩞࡛ሒ⛁Ꮥᐁ⩞ࢅ⾔࠹ࡒࡴࡡᩒങᚪさ࡚࠵ࡾࠊࡐࡡࡒࡴࡡࢪ࣭࣋ ࢪ࡛スങങဗࡡᑙථࠉࡐࡿࡼࢅ⥌ᣚ⟮⌦ࡌࡾ∸ⓏࠉெⓏࢦ࣭࣎ࢹྊḖ࡚࠵ ࡾࠊࡈࡼ࡞ࠉᏕ⛁ス❟ᚃࡵࠉሒⓆಘࡷ❿ணⓏ㈠㔘ࡡ⋋ᚋࢅྱࡴࡒ㗞ណ⤽⤾Ⓩ ຑງࢅ⾔࠷ࠉࡻࡽⰃ࠷◂✪࣬ᩅ⫩⎌ሾࡡᵋ⠇ࢅ┘ᣞࡌࠊ 16 㸭㸣⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩ࣈࣞࢡ࣑ࣚ㸝:5'8'㸞ࡡ⌟≟ 㸭㸣㸦 ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩ࣈࣞࢡ࣑ࣚࡡᴣさ ㎾ᖳࡡࣁ࢛ࣤࣆ࢚࣏ࢷࢠࢪࡡெᮞ㣬ᠺࢅịࡴࡾኇ࡞ᚺ࠻ࡾࡵࡡ࡛ࡊ࡙ࠉ ᮶ாኬᏕ⌦Ꮥ㒂࡞࠽࠷࡙ࠉᖲᠺ㸦㸨ᖳ㸦㸥᭮࠾ࡼᖲᠺ㸦㸭ᖳ㸨᭮ࡱ࡚ࠉᩝ㒂⛁ Ꮥ┤⛁Ꮥᢇ⾙ᣲ⮾ㄢᩒ㈕㸝᩺⮾ฦ㔕ெᮞ㣬ᠺ㸞࡞ࡻࡽ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩≁ ืࣈࣞࢡ࣑ࣚ㸝:5'8'":QIHUJUFIXFWH5URJUFPIRU'LRLQIRUPFWLHVFQI8^VWHPV 'LRORJ^㸞ᐁ᪃ࡈࡿࡒࠊࡆࡡࣈࣞࢡ࣑࡚ࣚࡢࠉ㸨ᖳ⏍┞ᙔ⛁┘ࡢኚఆࡲࢅฺ⏕ ࡊ࡙⾔ࢂࡿࠉ㸩ᖳ⏍┞ᙔ⛁┘ࡢࠉᖲ㸫㝀࡛ᅰ᭑ࢅฺ⏕ࡊ࡙⾔ࢂࡿࡒࠊ ࡐࡿ࡞ࡵ㛭ࢂࡼࡍࠉᐁ㈻㸩ᖳ㛣࡚㏳ᖏࡡᏕ⛁ࡲࡡ⣑㸪㸥ྞࡡಞ⏍༛ᴏࡊࠉ ⏍∸ሒ⛁Ꮥᴗࡴ࡙㨡ງⓏ࡚㞺さᗐ㧏࠷Ꮥၡ㡷ᇡ࡚࠵ࡾࡆ࡛ࢂ࠾ࡖࡒࠊ ࡆࡡࡻ࠹ᠺᯕࢅཬ᫆ࡊࠉᖲᠺ㸦㸮ᖳᗐ࠾ࡼࡢ⏍∸ሒ⛁Ꮥ⛁⌦Ꮥ㒂ࡡḿᘟ Ꮥ⛁࡛ࡊ࡙⏍ࡌࡾࠊ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩ࣈࣞࢡ࣑ࣚࡢࠉ⏍∸ሒ⛁ᏕᏕ 㒂ᩅ⫩≁ืࣈࣞࢡ࣑ࣚࡡྞ⛘ࢅⱕᖱን᭞ࡊ࡙㸝≁ืࢅ๎㝎ࡊࡒ㸞ࠉ⏍∸ሒ⛁Ꮥ Ꮥ㒂ᩅ⫩ࣈࣞࢡ࣑ࣚ:'58'࡛ࡊ࡙ࠉᖲᠺ㸦㸭ᖳᗐ࠾ࡼ᩺ࡒ࡞ス⨠ࡈࡿࡒࣈࣞ ࢡ࣑࡚ࣚ࠵ࡽࠉ᩺Ꮥ⛁࡞㸨ᖳ⏍㐅Ꮥࡌࡾࡱ࡚ࡡ㸨ᖳ㛣ᐁ᪃ࡈࡿࡾࠊᖲᠺ㸦㸭 ᖳᗐ࡞ࡢ㸧㸦ྞࡡಞ⩽ࢅ㍦ฝࡊࡒࠊ⏍∸ሒ⛁Ꮥ⛁ࡡᑍ௴ᩅဤ㸝⌦Ꮥ⣌◂✪ ⛁⏍∸Ꮥᑍᨯ㸧ྞࠉ᩺㡷ᇡᠺ⛁Ꮥ◂✪⛁ሒ⏍⛁Ꮥᑍᨯ㸦ྞࠉሒ⌦ᕝ Ꮥ⣌◂✪⛁ࢤࣤࣅ࣭ࣖࢰ⛁Ꮥᑍᨯຐᡥ㸦ྞ㸝ᖲᠺ㸦㸮ᖳᗐ࠾ࡼ㸞㸞࡛ࠉ᩺㡷ᇡ ᠺ⛁Ꮥ◂✪⛁ሒ⏍⛁Ꮥᑍᨯࡡ⌟ᩅဤ㸝 ྞ㸞ࢅ୯ᚨ࡛ࡊࠉࡈࡼ࡞⌦Ꮥ⣌◂✪ ⛁⏍∸Ꮥᑍᨯ࠽ࡻࡦሒ⌦ᕝᏕ⣌◂✪⛁ࡡᩅဤ࠾ࡼᠺࡾ⤄⧂࡚࠵ࡾࠊ 㸭㸣㸧 ᑍ㛓ᩅ⫩ࡡහᐖ ㅦ⩇⛁┘ࡢࠉ⏍⛁Ꮥࠉሒ⛁Ꮥࠉࡐࡊ࡙ࣁ࢛ࣤࣆ࢚࣏ࢷࢠࢪ࣬ࢨࢪࢷ ࣑⏍∸Ꮥࡡ㸨ᮇ❟࡙࡚ᵋᠺࡈࡿ࡙࠷ࡾࠊ:5'8'㸧ᩅဤ࡞ຊ࠻ࠉᏕහአࡡ➠ୌ⥲࡚ Ὡ㌅ࡈࡿ࡙࠷ࡾㅦᖅࡡ༝ງ࡞ࡻࡽᐁ᪃ࡈࡿ࡙࠷ࡾࠊ㸨ᖳ┞ᙔ⛁┘࡚࠵ࡾㅦ⩇ ⛁┘࡛ሒ⛁Ꮥᐁ㥺 ⛁┘ࠉࡐࡊ࡙㸩ᖳ┞ᙔ⛁┘ࡡㅦ⩇ ⛁┘࠵ࡽࠉධ࡙ḿ ᘟ࡞༟న࡛ࡊ࡙ヾࡴࡼࡿࡾ⌦Ꮥ㒂ධᏕ⛁භ㏳⛁┘࡚࠵ࡽࠉモ㥺࡞ࡻࡽᠺ⦴ࡗ ࡄࡼࡿࡾࠊ⌦Ꮥ㒂ࡡࡲࡼࡍ᮶ாኬᏕࡡධᏕ⏍ࠉࡈࡼ࡞ࡢ♣ఌெ࡞ࡵ㛓ᡖࢅ㛜 ࠷࡙࠽ࡽࠉᒓಞᒀࢅᥞฝࡌࡾࡆ࡛࡞ࡻࡽུㅦ࡚ࡀࡾ⤄ࡲ࡛ࡖ࡙࠷ࡾࠊ 17 㸭㸣㸨 ᩅဤᵋᠺ ⌦Ꮥ⣌◂✪⛁⏍∸Ꮥᑍᨯ⏍∸ሒ⛁ᏕᏕ㒂ᩅ⫩ࣈࣞࢡ࣑ࣚ 㯦⏛ ┷ஒ ᩅ ⛤ ⨶Ꮔ ຐᩅ ሒ⌦ᕝᏕ⣌◂✪⛁ࢤࣤࣅ࣭ࣖࢰ⛁Ꮥᑍᨯ ⏛୯ ᩝ ຐᡥ㸝ᖲᠺ㸦㸮ᖳ㸩᭮ࡻࡽ㸞 ᩺㡷ᇡᠺ⛁Ꮥ◂✪⛁ሒ⏍⛁Ꮥᑍᨯ 㧏ᮄ ฺ ᩅ ୖ ┷ୌ ᩅ ⸠ 㝧ྒྷ ᩅ ὰ ₡ ᩅ ᭱㒂 ḿᖲ ᩅ㸝ᖲᠺ㸦㸭ᖳ㸬᭮ࡻࡽ㸞 ᭯⏛ ḿぞ ຐᩅ ୯ㆺ ᪺ᘧ ຐᩅ ⌦Ꮥ⣌◂✪⛁⏍∸Ꮥᑍᨯ ᶋᒜ Ⱪ ᩅ ᴦ ᗛᩝ ຐᩅ 㸭㸩ᒓಞ࡛ಞッ࡞ࡗ࠷࡙ ᮇࣈࣞࢡ࣑ࣚࡢࠉᏕ㒂㸨ᖳ┞ᙔ⛁┘࡛Ꮥ㒂㸩ᖳ┞ᙔ⛁┘࠾ࡼࡽࠉᏕ㒂㸨ᖳ┞ ᙔ⛁┘ࡡ࠹ࡔ㸦 ༟న࡛ᐁ㥺㸦⛁┘㸧༟నࢅᏕಞࡊࠉᏕ㒂㸩ᖳ┞ᙔ⛁┘ࡡ࠹ࡔ⏍ ࢨࢪࢷ࣑Ϩ࡛ϩࢅ㝎ࡂ㸦㸥༟నࢅᏕಞࡊࡄࡿࡣࡼ࠷ࠊ ࡱࡒࠉᮇࣈࣞࢡ࣑ࣚぞ์࡞ᏽࡴࡾ༟నᩐࢅᏕಞࡊࡒ⩽࡞ࡢࠉ⌦Ꮥ㒂㛏ࡻࡽಞ ッࡈࡿࡾࠊ࠽ࠉಞッࡡ࡞㝷ࡊ࡙ࡢࠉᮇࣈࣞࢡ࣑ࣚࡡ⛁┘ࡡ༟న ࢅ⌦Ꮥ㒂ࡡࡡ㛜ㅦ⛁┘ࡡ༟న࡞ࡻࡖ࡙᭨ࡌࡾࡆ࡛࡚ࡀࡾࠊ 㸭㸣㸪 ᖲᠺ㸦㸭ᖳᗐධᏕ⛁භ㏳⛁┘ ᖳ┞ᙔ⛁┘ ࣬㒼าሒよᯊᏕ㸯 )3&࣬73&ࡡሲᇱ㒼าࠉ࣐ࢿ㓗㒼าࡡሒよᯊἪࢅよㄕ ࡌࡾࠊ㒼า࣒ࣚࣤࣤࢹࠉࢸ࣭ࢰ࣭࣊ࢪࡡ┞ྜྷᛮ᳠⣬ࠉ㐿ఎᏄⓆぜࠉ࣐ࢿ 㓗㒼า୕ࡡᶭ⬗࣓ࢲ࣭ࣆࡷࢨࢡࢻࣜࡡ᥀⣬ࢅཱིࡽ࠹ࠊ 18 㞗୯ㅦ⩇ࠉ㹳ࠉ 㝀㹳 㝀㹳ࠉ⌦ ࣬ᩐ⌦⤣ゝᏕ㸯 ࢣࢿ࣑࣬㐿ఎᏄ㛭㏻◂✪࠽ࡻࡦ⮣ᗃ࣬Ꮥ◂✪࡚⏕࠷ࡼࡿࡾ ⤣ゝᡥἪࢅᴣふࡊࠉㄵᩝࢅ⌦よࡌࡾࡒࡴ࡞ᚪさᩐ⌦Ⓩᇱ♇࡛よᯊᢇἪࢅㅦ⩇ ࡌࡾࠊࡱࡒࣃࢹࢣࢿ࣑よᯊࡡ᭩❻ࡡࢹࣅࢴࢠࡵㅦ⩇ࡌࡾࠊ㡧┘☔⋙ንᩐ ࡛☔⋙ฦᕱࠉᵾᮇฦᕱࡡᴣᛍࠉ᥆ᏽ࡛᳠ᏽࠉ࣓ࢸࣜࡡᙔ࡙ࡢࡴ࡛ᑦᗐࠉ࣊ࢫ ⤣ゝࠉኣ⩄ࡡẒ㍉ࠉᅂᖉ࡛┞㛭ࠉᅂᖉ࣓ࢸࣜࡡᣉᘿࠉኣን㔖よᯊࠉࣃࢹࢣࢿ࣑ よᯊ 㞗୯ㅦ⩇ࠉ㹳ࠉ 㝀㹳 㝀㹳ࠉࠉ 㝀㹳ࠉ ⌦ ࣬⏍మฦᏄ㐿ఎᏄሒᏕ㸯 ᮇㅦ⩇ࡢࠉ⏍⛁Ꮥࡡᇱ♇Ⓩㅦ⩇࡛ࡊ࡙ࠉ࡞ ⏍⣌Ꮥ⏍௧አࢅᑊ㇗࡛ࡊ࡙⾔࠹ࠊ㐿ఎᏄࡡᵋ㏸࡛々⿿ࠉ㐿ఎᏄⓆ⌟࡛ࡐࡡโ ᚒࠉࢨࢡࢻࣜఎ㐡ࠉ⣵⬂࿔࡛⣵⬂ฦࠉ⣵⬂ᵋ㏸࡞ࡗ࠷࡙ࠉࠔࣛࣤ㓗࣬ ⬲ࣛࣤ㓗ࠕࠉࠔࢰࣤࣂࢠ㈻㛣┞பష⏕ࠕ࡛࠷ࡖࡒ⣵⬂හሒࡡᇱᮇⓏさ⣪࠾ࡼ よㄕࡌࡾࠊࡱࡒࠉࡻࡽ࣏ࢠࣞ⏍⌟㇗ࠉࡒ࡛࠻ࡣࠉ⣵⬂Ṓࠉ⣵⬂ቌṢ࡛ᙟ㈻ ㌹ᥦ⒬ࠉአ㒂វて⣌វࡡ⌟㇗࡛୕オࡡᇱᮇⓏさ⣪࡛ࡡ㛭㏻ࡘࡄࢅ ⾔࠷ࠉฦᏄິឺ࠾ࡼ⏍⌟㇗࡞⮫ࡾ㐠➵ࢅᏕࡩࡆ࡛ࢅࡴࡉࡊ࡙࠷ࡾࠊ 㞗୯ㅦ⩇ࠉ㹳ࠉ 㝀㹳 㝀㹳ࠉ⌦ ࢛࣭࣐࣬ࢴࢠࢪㄵ㸯 ࢣࢿ࣑ࢦ࢙ࣤࢪࡡ᭩ኬࡡ≁ᚡࡢ⥑⨮Ⓩ࣬⤄⧂Ⓩよᯊ࡞ ࠵ࡽࠉࢅ࣑࣭࢛ېېよᯊࡌࡾࡆ࡛࠾ࡼࠔ࢛࣭࣐ࢴࢠࢪࠕ࡛ࡵࡣࡿࡾࠊᮇㅦ⩇ ࡚ࡢࠉࢣࢿ࣑ࡡ㒼าỬᏽ࡛ࢿࢷ࣭ࢨ࡛ࣘࣤ࠷࠹ࢣࢿ࣐ࢠࢪࡡᇱ♇࠾ࡼࠉẒ㍉ ࢣࢿ࣑よᯊࡷ࣒ࢰࢣࢿ࣑よᯊࠉ᭞࡞ࢹࣚࣤࢪࢠࣛࣈࢹ࣭࣑ࠉࣈࣞࢷ࢛࣭࣑ࠉࣆ ࢘ࢿ࣭࣑ࢅ࠹ᶭ⬗ࢣࢿ࣐ࢠࢪࡱ࡚ࠉᵕࠍࠔ࢛࣭࣐ࢴࢠࢪࠕࢅ⣺ࡌࡾࠊ 㞗୯ㅦ⩇ࠉ㹳ࠉ 㝀㹳 㝀㹳ࠉ⌦ ࣬⏍∸ሒࢮࣆࢹ࢙ㄵ㸯 ᇱᮇⓏࢸ࣭ࢰᵋ㏸࡛ࣜࢥࣛࢫ࣑ࢅよㄕᚃ ࡞ࠉኬぞᶅ )3& 㒼าࢸ࣭ࢰࡡ㧏㏷ฌ⌦Ἢࠉࢦࣆࢴࢠࢪ࣭ࣝࠉࢨ࣭ࢺࣚ ࣒ࣤࣤࢹࠉࢣࢿ࣑ࢬࣤࣇࣛࠉࣈ࣏ࣚ࣬)3& ࢲࢴࣈ࣬VL73& 㒼าスゝࠉ࢛ࣆࢰ ࣭ࢣࢴࢹ᳠⣬ࢅཱིࡽ࠹ࠊᮇㅦ⩇ࡡහᐖࡢ⏍∸ሒ⛁Ꮥᐁ㥺 . ࡡⁿ⩞ㄚ㢗 ࡛ࡾࡒࡴࠉྜྷᐁ㥺ࡵ࠵ࢂࡎ࡙ᒓಞࡊ⏍∸ሒࢮࣆࢹ࢙ࢅషᠺࡌࡾࡆ࡛ 19 ᭻ࡱࡊ࠷ࠊ 㞗୯ㅦ⩇ࠉ㹳ࠉ 㝀㹳 㝀㹳ࠉ⌦ ࣬⏍∸ᵋ㏸⛁Ꮥ㸯 ᵋ㏸ࢣࢿ࣑⛁Ꮥࡡᇱ♇࡛ࡾ⏍మ㧏ฦᏄࡡᵋ㏸よᯊἪࠉ࠽ ࡻࡦᵋ㏸⏍∸Ꮥ࡞ࡗ࠷࡙よㄕࡌࡾࠊරమⓏ࡞ࡢࠉࢰࣤࣂࢠ㈻࣬ᰶ㓗ࡡ㧏ḗᵋ㏸ࠉ = ⥲⤎ᬏཀྵࡦ 327 ࡞ࡻࡾ⏍మ㧏ฦᏄᵋ㏸よᯊࡡᇱ♇ࠉฦකῼᏽࠉ⇍ງᏕࠉ㧏ฦᏄ ⁈ᾦㄵཀྵࡦཬᚺ㏷ᗐㄵࢅᏕࡦࠉ⏍మ㧏ฦᏄࡡᵋ㏸࡛ᶭ⬗㛭ࡌࡾ⏍∸ᏕⓏᶭ ⬗࡞ࡗ࠷࡙㏑ࡾࠊ⌦ㄵゝ⟤Ἢ࡞࠽࠷࡙ࡢࠉ࣐ࢿ㓗㒼า࣒ࣚࣤࣤࢹࠉᵋ ㏸࣒ࣚࣤࣤࢹࠉḗᵋ㏸ῼཀྵࡦ❟మᵋ㏸ῼࠉᵋ㏸ฦ㢦ࠉᵋ㏸ࢸ࣭ࢰ ࣭ࢪࢅཱིࡽ࠹ࠊ᭞࡞ࠉ࣎ࢪࢹࢣࢿ࣑⛁Ꮥࡡࡡࢼ࣭ࢫ࡞ྙࢂࡎࡒࣀ ࢪ࣭ࣜࣈࢴࢹᵋ㏸よᯊ࡞ࡗ࠷࡙ࡵゕཀྵࡊࠉᵋ㏸よᯊࡡ⤎ᯕࡡࡻ࠹⏍∸⌟ ㇗ࢅ᪺ࡼ࠾࡞ࡊ࡙ࡀࡒ࠾ࠉ࡛࠷ࡖࡒᵋ㏸⏍∸Ꮥ࡞ࡗ࠷࡙ࡵよㄕࡌࡾࠊ 㞗୯ㅦ⩇ࠉ㹳ࠉ 㝀㹳 㝀㹳ࠉ⌦ ࣬ࢨࢪࢷ࣑⏍∸Ꮥ㸯 ⏍∸ሒ⛁Ꮥࡡ❟ሔ࠾ࡼࠉ⏍⌟㇗ࢅ࣓ࢩ࣭ࣖࣜࡊࡒ ᙟ࡚⾪⌟ࡊࠉ⏍ࢅࢨࢪࢷ࣑࡛ࡲࡊ࡙よᯊࡌࡾᡥἪࢅ⣺ࡌࡾࠊࢨࢪࢷ࣑⏍ ∸Ꮥࡢ㟸ᖏ࡞ᗀ࠷ㄊ⩇ࢅᣚࡗゕⴝࡓࠉ⾪ౚ࡛ࡊ࡙ᐁ㥺࡚ᚋࡼࡿࡒ㐿ఎᏄࡡ Ⓠ⌟ࣂࢰ࣭ࣤࢅ⏕࠷ࡒ㌹โᚒࢾࢴࢹ࣭࣠ࢠࡡᶭᏕ⩞ࠉㅨ⏍మහࡡࢾ ࢴࢹ࣭࣠ࢠࢅࢸ࣭ࢰ࣭ࢪࡌࡾᢇἪࠉࢨࢡࢻࣜఎ㐡⤊㊪ࡡゝ⟤ᶭࢨ࣐࣭ࣖࣝ ࢨࣘࣤࢅ⏕࠷ࡒ㟸⥲ᙟᚺ➽ࡡよᯊ᪁Ἢ࡞ࡗ࠷࡙ㅦ⩇ࢅ⾔࠹ࠊ 㞗୯ㅦ⩇ࠉ㹳ࠉ㹳 㝀㹳ࠉ⌦ ࣬ሒ⛁Ꮥᐁ㥺 .㸯 ᮇᐁ㥺࡚ࡢ௧ୖࡡ ࡗࡡㄚ㢗࡞ཱིࡽ⤄ࡳࠊ ኬぞᶅ )3& 㒼าỬᏽࡡ⤄ࡲࢅ⌦よࡌࡾࡒࡴࡡࣈࣞࢡ࣑ࣚ⿿ష ࣈࢲࢺ᩷∞࠾ࡼᮇᮮࡡࢰࣤࣂࢠ㈻ࢅྜྷᏽࡌࡾࢸ࣭ࢰ࣭ࢪ᳠⣬ࣈࣞࢡࣚ ࣑ࡡషᠺ 㐿ఎᏄⓆ⌟㔖ࢅよᯊࡌࡾᶭᏕ⩞ࣈࣞࢡ࣑ࣚࢠࣚࢪࢰࣛࣤࢡࢠࣚࢪฦ 㢦ࡡషᠺ ⏍Ꮥཬᚺ࡞ᇱࡘ࠷ࡒࢨ࣐࣭ࣖࣝࢨ࣓ࣘࣤࢸࣜࡡషᠺ 㹳レ⣵ࡢ -5 ࡞᥎㍍ࠉ 㝀㹳㹳ࠉ⌦ ᏽ 㸩ᖳ┞ᙔ⛁┘ 20 ࣬⏍ࢨࢪࢷ࣑ .㸯 ࢣࢿ࣑ࢅぞᏽࡊ࡙࠷ࡾゕㄊࠉ⏍ࢨࢪࢷ࣑࡞හᅹࡊ࡙࠷ࡾ ゕㄊⓏᵋ㏸ࠉ⏍⛁Ꮥ◂✪⩽▩ㆉࢅオ㏑ࡌࡾ⮤↓ゕㄊࠉ⏍ࢨࢪࢷ࣑ࢅࢨ࣐ ࣭ࣖࣝࢹࡌࡾࡒࡴࡡゝᶭゕㄊࡡ㸩ࡗࡡゕㄊ࡞╌┘ࡊࠉ⏍ࢨࢪࢷ࣑ࢅࠔㄖࡲよ ࡂࠕࡆ࡛ࢅ┘ᣞࡌ◂✪࡞ࡗ࠷࡙ㅦ⩇ࢅ⾔࠹ࠊ(4* ≁ืㅦ⩇ࠊ 㛜ㅦࠉẎ㐄ᮄ᭑ 㝀㹳 㝀㹳ࠉ⌦ ࣬⏍ࢨࢪࢷ࣑ ..㸯 ࢣࢿ࣑࡞㛭㏻ࡊࡒゕㄊ࡞࡛ࡱࡼࡍࠉ⏍ࢨࢪࢷ࣑࠽ࡻ ࡦゕㄊ࡞㛭㏻ࡌࡾᏕၡ࣬ᢇ⾙࡞ࡗ࠷࡙ࠉࡐࡡ⫴ᬊࡵྱࡴ࡙ᖕᗀ࠷❟ሔ࠾ࡼㅦ⩇ ࢅ⾔࠹ࠊ(4* ≁ืㅦ⩇ࠊ 㛜ㅦࠉẎ㐄ᮄ᭑ 㝀㹳 㝀㹳ࠉ⌦ ࣬ࢣࢿ࣑㐅Ꮥ㸯 ࡆࡡㅦ⩇࡚ࡢࠉ㎾ᖳࡡࢣࢿ࣑◂✪ࡡ㐅ᒈࢅ᳔∸ࢅ୯ᚨ࡞よ ㄕࡌࡾࠊභ⏍࡛࠷࠹ᙟ࡚⣵⬂හ࡞ථࡽ㎲ࢆࡓⴝ⥫మࡢࠉᐙ⣵⬂࡛༝ㄢࡊ࡙㐅 ࡊ࡙ࡀࡒ࡛⩻࠻ࡼࡿࡾࠊⴝ⥫మࡢ⮤ᕤࡡࢣࢿ࣑ሒࡡኣࡂࢅᐙ⣵⬂ࡡࢣࢿ ࣑࡞ᢰࢂࡎ࡙࠽ࡽࠉ୦⩽ࡡ㐿ఎᏄྜྷࡊ࡙Ⓠ⌟ࡌࡾโᚒᶭᵋ࠵ࡾ࡛⩻࠻ࡼ ࡿ࡙࠷ࡾࠊࡆࡡࡻ࠹㐿ఎᏄⓆ⌟ࡷᙟឺᙟᠺࡡโᚒ࡞ࡗ࠷࡙㐅ࡡふⅤ࠾ࡼよ ㄕࡌࡾࠊ ᅰࠉ 㝀㹳 㝀㹳ࠉᅰࠉ 㝀㹳 㝀㹳ࠉ⌦ ࣬Ẓ㍉ࢣࢿ࣑Ꮥ㸯 Ẓ㍉ࢣࢿ࣑Ꮥࡢࠉྜྷୌ⛸ࠉ㎾⦍⛸ࠉ㐪⦍⛸ࡡమࡷ㞗ᅆࢅ ᑊ㇗࡞ࢣࢿ࣑ࡡᵋ㏸ࡷᶭ⬗ሒࡡẒ㍉ࢅ⾔࠷ࠉࡈࡱࡉࡱっⅤ࠾ࡼ⏍ࢨࢪࢷ ࣑ࡡໜᣋⓏ⌦よࢅ┘ᣞࡌࠊࡐࡡࡒࡴ࡞ࡢࠉࢣࢿ࣑ሒ࡞㛭ࡌࡾᐁ㥺ࢸ࣭ࢰࢅ ཱིᚋࡌࡾࡒࡴࡡࣤࢲ࣭࣠ࢠᡥἪ࡞ຊ࠻ࠉࢸ࣭ࢰࡡよᯊ࡛よ㔐ࡡ⌦ㄵⓏ⫴ᬊ ࡛ࡾ⤣ゝ⌦ㄵ࡛ฦᏄ㐅⌦ㄵࢅ༎ฦ࡞⌦よࡌࡾᚪさ࠵ࡾࠊࡱࡍࠉ⏍∸⛸࡞ ࡢኣ㔖ࡡ㐿ఎⓏን␏⥌ᣚࡈࡿ࡙࠷ࡾࡆ࡛ࢅヾㆉࡌࡾᚪさ࠵ࡾࠊ⥌ᣚࡈࡿ࡙ ࠷ࡾ㐿ఎⓏን␏㔖ࢅ )3& 㒼าሒ࠾ࡼ᥆ᏽࡌࡾ⤣ゝἪࠉ᥆ᏽࡈࡿࡒ⤣ゝ㔖࠾ࡼ ర࠷࠻ࡾࡡ࠾࡞ࡗ࠷࡙よㄕࡌࡾࠊࡱࡒࠉẒ㍉ࢣࢿ࣑Ꮥ࡚⏕࠷ࡼࡿࡾᵕࠍよ ᯊἪ┞ྜྷᛮ᳠⣬ࠉኣ㔔ᩒาࠉ⣌⤣ᶖషᠺࠉྜྷ⩇㟸ྜྷ⩇⨠ᥦ㏷ᗐࡡ㐅㏷ᗐ ࡡ᥆ᏽࢅࠉ㐅ࡡㅎ┞࡞ࡗ࠷࡙ㄵࡋࡗࡗㄕ᪺ࡌࡾࠊ௧୕ࡡ⌦ㄵⓏ⫴ᬊࢅࡵ ࡛࡞ࠉ᭩᩺ࡡẒ㍉ࢣࢿ࣑◂✪ࡡᠺᯕࢅㄵࡋ࡙ㅦ⩇ࡡ⥶ࡴࡂࡂࡽ࡛ࡌࡾࠊ ᅰࠉ 㝀㹳 㝀㹳ࠉᅰࠉ 㝀㹳 㝀㹳ࠉ 21 ⌦ ࣬㧏㏷ฦᏄࢨ࣐࣭ࣖࣝࢨࣘࣤ㸯 ⏍⛁ᏕࡡⓆᒈ࡞ࡻࡽࠉ⏍ࢨࢪࢷ࣑ࡡධమ Ⓩ⌦よっ㔕࡞ථࡽࡗࡗ࠵ࡾࠊ々㞟ኣమ㟸⥲ᙟ⣌࡚࠵ࡾ⏍ࡡ⌦よ࡞ࡢࠉゝ ⟤ᶭ࡞ࡻࡾࢨ࣐࣭ࣖࣝࢨࣘࣤ㔔さࢵ࣭࡛ࣜࡾ࡚࠵ࢀ࠹ࠊᮇㅦ⩇࡚ࡢฦᏄ ࢨ࣐࣭ࣖࣝࢨࣘࣤ࣬ࢾࢴࢹ࣭࣠ࢠࢨ࣐࣭ࣖࣝࢨࣘࣤ࡞ࡻࡾ⏍మฦᏄ㛣ࡡ┞பష ⏕ࢅよ᪺ࡡᡥἪ࡞ࡗ࠷࡙よㄕࡊࠉ㧏ᛮ⬗ゝ⟤࡞ࡻࡾᑑᮮࡡྊ⬗ᛮ࡞ࡗ࠷࡙ㄵࡍ ࡾࠊ 㹳ࠉẎ㐄Ề᭑ 㝀㹳ࠉ⌦ ࣬⺦Ⓣ㈻ ḗඔᵋ㏸ῼ㸯 ⺦Ⓣ㈻ ḗᵋ㏸ῼ࡞㛭ࡌࡾ⤣ゝよᯊࡷ∸⌦Ꮥ ⓏᡥἪࡡᇱ♇Ⓩ⌦よ࡛ᐁ㊰ࣝ࣊ࣜࡡῼࣈࣞࢹࢤࣜࡡ⩞ᚋࢅ┘Ⓩ࡛ࡌࡾࠊㅦ⩇ ࡢࠉ⺦Ⓣ㈻ ḗᵋ㏸ῼࡡ⫴ᬊࠉẒ㍉࣓ࢸࣛࣤࢡࠉᵋ㏸ヾㆉἪࠉ㗢ᆵࢅฺ⏕ࡊ ࠷࣓ࢸࣛࣤࢡἪ⾪Ⓩ᪁Ἢㄵࡡᴣㄕ࡛㛭㏻ࡌࡾᇱ♇よᯊࡡ⣺ࢅ୯ᚨ ࡛ࡊࡒහᐖ࡛ࡌࡾࠊࡱࡒࠉㅦ⩇ࡡᚃ༖࡚ࡢࠉῼᡥ㡨ࡡᐁ㝷࡛ၡ㢗Ⅴࠉᚺ⏕ ౚࡵ⣺ࡌࡾࠊ ᅰࠉ 㝀㹳 㝀㹳ࠉᅰࠉ 㝀㹳 㝀㹳ࠉ ⌦ ࣬ࣁ࢛ࢸ࣭ࢰ࣏ࢼࣤࢡ㸯 Ⓠ⌟㔖ࢠࣚࢪࢰࣛࣤࢡࠉ691 よᯊࠉ㐿ఎᏄᶭ⬗ ῼࡡࡒࡴࡡᶭᏕ⩞ᡥἪࡡཋ⌦ࢦ࣭࣎ࢹ࣊ࢠࢰ࣭࣏ࢨࣤࠉ'RRVWLQJࠉỬᏽᮄࠉ ㎾ⅤἪࠉࣁ࢛࣒࣭ࢩࣤࢡ࡛⥑⨮Ⓩ⾪⌟ᆵよᯊࣆ࢘ࢿ࣭࣑よᯊ➴ࡡ᭩ ❻ࡡリ㢗ࢅㅦ⩇ࡌࡾࠊࣈࣞࢡ࣑ࣚᥞ౩࡞ࡻࡾⁿ⩞ࡵୌ㒂࠽ࡆ࠹ࠊ 㛜ㅦࠉẎ㐄ᮄ᭑ 㝀㹳ࠉ⌦ ࣬⏍ሒ⾪⌟㸯 ⏍◂✪ࢅຝ⋙Ⓩ࡞㐅ࡴࡾࡒࡴ࡞ࡢࠉࢣࢿ࣑㒼าࡡᐁ 㥺ࢸ࣭ࢰࡓࡄ࡚ࡂࠉࣂࢪ࢘ࠉ⾪⌟ᆵࡡ▩ㆉࢅࢸ࣭ࢰ࣭࣊ࢪࡌࡾࡆ ࡛Ḗ࠾ࡎ࠷ࠊᮇㅦ⩇࡚ࡢࠉࢸ࣭ࢰ࣭࣊ࢪࡡ㛜Ⓠິྡྷ࡛ࠉࡐࡡᇱ┑ᢇ⾙࡚࠵ ࡾࠉࢸ࣭ࢰ࣭࣊ࢪᇱ♇ㄵࠉ࢛ࣤࢹࣞࢩ࣭⏍∸ᶭ⬗ࡡ⾪⌟Ἢࠉࣂࢪ࢙ࡡ⾪ ⌟Ἢ࡛ࢨ࣐࣭ࣖࣝࢨࣘࣤࠉᩝ⊡࠾ࡼࡡ▩ㆉฝ࡞ࡗ࠷࡙Ꮥ⩞ࡌࡾࠊ ࠉࠉᅰࠉ 㝀㹳 㝀㹳ࠉᅰࠉ 㝀㹳 㝀㹳 ࠉ⌦ 22 ࣬ᩐ⌦⏍∸Ꮥ㸯 ♼⤊⣌ࡡᩐ⌦࣓ࢸࣜࢅ⛸ࠍࡡ❟ሔ࠾ࡼㄵࡍࡾࠊ࣌ࢩ࢞ࣤ࣬ࣀ ࢠࢪࣝ᪁⛤ᘟࠉࢼ࣭ࣖࣚࣜࢾࢴࢹ࣭࣠ࢠ࣓ࢸࣜ࠾ࡼฝⓆࡊ࡙ࠉࢾࢴࢹ࣭࣠ࢠ ࡡࢱࢻ࣐ࢠࢪࠉ࢛࢜ࢪࠉࣆࣚࢠࢰࣜࠉ々㞟⣌ࠉࡈࡼ࡞ࡢ⏍⌦ᏕⓏጂᙔᛮ ࡡリ㢗࡞ࡵࡨࡿࡾࠊ ࠉࠉᅰࠉ 㝀㹳 㝀㹳ࠉᅰࠉ 㝀㹳 㝀㹳ࠉ ⌦ 23 㸮㸣◂✪Ὡິ 㸮㸣㸦 ◂✪හᐖ 㸮㸣㸦㸣㸦ࢨࢡࢻࣜఎ㐡ᶭᵋࡡሒࢤ࣭ࢸࣤࢡ ቌṢࡷฦ࡛࠷ࡖࡒ⏍⌟㇗ࡢ⣵⬂හฦᏄࢾࢴࢹ࣭࣠ࢠ࡚࠵ࡾࢨࢡࢻࣜఎ㐡ᶭ ᵋ࡞ࡻࡽโᚒࡈࡿ࡙࠷ࡾࠉࡐࡡ⛸㢦ࡢථງๆ⃥ࡷ⣵⬂ࡡᚺ➽࡞Ẓࡾ࡛ᑛ ࡂ㝀ࡼࡿ࡙࠷ࡾ㹶ࢨࢡࢻࣜఎ㐡ᶭᵋࡡᮇ㈻ࡢࠉኣᙤථฝງࢅ㝀ࡼࡿࡒ⛸㢦ࡡ⣵ ⬂හฦᏄ࡞ࢤ࣭ࢺࡌࡾࡆ࡛࡞࠵ࡾࠊࡆࡡࢤ࣭ࢸࣤࢡ࡞ࡢࠉථງࡡ㐢࠷ࢅὩᛮ ࡌࡾฦᏄࡡ⤄ࡲྙࢂࡎ࡞ࢤ࣭ࢺࡌࡾ௧አ࡞ࡵฦᏄὩᛮࡡ㛣ἴᙟ࡞ሒࢅࢤ ࣭ࢺࡌࡾ㛣ሒࢤ࣭ࢸࣤࢡ࠵ࡾࠊᮇ◂✪࡚ࡢࠉሒࢤ࣭ࢸࣤࢡࡡふⅤ ࠾ࡼࠉྜྷࡋๆ⃥࡚࠵ࡖ࡙ࡵ㛣ἴᙟ࡞౪Ꮛࡊ࡙␏ࡾᚺ➽ࢅ♟ࡌ⏍⌟㇗࡚࠵ ࡾ⣵⬂㐘Ửᏽ࡛ࣤࢪࣛࣤష⏕࡞ࣆ࢚࣭࢜ࢪࡊ࡙ࠉᐁ㥺࡛᚜ฦ᪁⛤ᘟ࣓ࢸࣜ ࢅ⤄ࡲྙࢂࡎ࡙㛣ሒࢤ࣭ࢸࣤࢡࡡ࣒࢜ࢼࢫ࣑ࢅよ᪺ࡌࡾࠊࡱࡒࠉ⣵⬂⛸ ࡞౪Ꮛࡊ࡙ฦᏄࡡⓆ⌟㔖␏ࡾࡒࡴࠉࡆࡿࡼࡡๆ⃥࡞ᑊࡌࡾᚺ➽ࡢ⣵⬂⛸ ࡞ࡻࡖ࡙ࡵ␏ࡾࠊࡐࡡ⤄ࡲࢅ⣵⬂⛸࡚ࡡⓆ⌟㔖ࡷᚺ➽ࡡふῼ࡛⤣ゝⓏ࣓ ࢸࣜࢅ⏕࠷࡙᪺ࡼ࠾࡞ࡌࡾࠊࡆࡿࡼࡡ࣓ࢸࣜࢅ⦨⣑ࡊ࡙ࢨࢡࢻࣜఎ㐡ࡡሒࢤ ࣭ࢸࣤࢡࡡᇱᮇཋ⌦ࢅฝࡌࡾࠊ ERK ⤊㊪ࡡࢸࢤ࣭ࢺ 5( ⣵⬂࡞࠽࠷࡙ 3,+*,+ ๆ⃥ࡡࢪࢷࢴࣈࠉࣚࣤࣈࠉࣤࣂࣜࢪๆ⃥ࢅຊ࠻࡙ *70 ࡡୌ㐛ᛮ࡛ᣚ⤾ᛮࡡἴᙟࢅᏭᏽ࡞షᠺ࡚ࡀࡾ᮪௲ࢅ᳠ゞࡌࡾࠊࡈࡼ࡞ࠉࡐࡿ ࡼࡡๆ⃥ࢅ⏕࠷࡙ࠉୌ㐛ᛮ࡛ᣚ⤾ᛮࡡ *70 ࡡὩᛮ࡞≁␏Ⓩ࡞ᚺ➽ࡌࡾ㐿ఎᏄ ⩄ࢅ࣏ࢠࣞࣝࢅ⏕࠷࡙ྜྷᏽࡌࡾࠊࡐࡡ๑ṹ㝭࡛ࡊ࡙ 3,+ ࡡࢪࢷࢴࣈࠉࣚ ࣤࣈࠉࣤࣂࣜࢪๆ⃥࡞ࡻࡾฦㄇᑙࢅ♼⤊✲㉫ࡡ㛏ࡈࢅᣞᵾ࡛ࡊ࡙᳠ゞࡊࡒࠊ ࡐࡡ⤎ᯕࠉ*70 ࡡୌ㐛ᛮ࡛ᣚ⤾ᛮࡡὩᛮࢅㄇᑙࡌࡾ 3,+ ࡡࢪࢷࢴࣈࡡ᪁ࠉ*70 ࡡᣚ⤾ᛮࡡὩᛮࡡࡲࢅㄇᑙࡌࡾࣚࣤࣈๆ⃥ࡻࡽ♼⤊✲㉫ࡡ㛏ࡈࢅຝ⋙ࡻࡂㄇ ᑙ࡚ࡀࡾࡆ࡛ࢅぜฝࡊࡒࠊࡱࡒ *70 ࡡୌ㐛ᛮࡡὩᛮࡡࡲࢅㄇᑙࡌࡾ 3,+ ࡡ ࣤࣂࣜࢪๆ⃥࡚ࡢ♼⤊✲㉫ࡡఘࡦࡢふᐳࡈࡿ࠾ࡖࡒࠊࡊࡒࡖ࡙ࠉ♼⤊✲㉫ ࡡຝ⋙ࡻ࠷㐅ᒈ࡞ࡢ *70 ࡡୌ㐛ᛮ࡛ᣚ⤾ᛮࡡὩᛮࡡ୦᪁┞Ⓩຝᯕࢅࡵ ࡒࡼࡊ࡙࠷ࡾࡆ࡛♟ြࡈࡿࡒࠊࡱࡒࠉ௧ୖࡡ ERK ⤊㊪ࡡ⤣ゝ࣓ࢸࣜᵋ⠇࡚⏕ 24 ࠷ࡾୌ⣵⬂ࣝ࣊ࣜࡡኣ㔔ඞⰅ࡞ࡻࡾ⣌าࢸ࣭ࢰࢅ㧏㏷࠾ࡗኬ㔖࡞ཱིᚋࡌ ࡾᡥἪࡡ㛜ⓆࢅモࡲࡒࠊරమⓏ࡞ࡢࡱࡍࡢࠉ*70 ࡡࣛࣤ㓗ࢅᣞᵾ࡞ඞ Ⰵࡡ㛣ἴᙟᖲᆍ \HVWHUQGORW ࡡ⤎ᯕ࡛ᩒྙᛮࡡཱིࡿࡾ᮪௲ࡡスᏽ࡞ᠺຉࡊ ࡒࠊࡆࡿ࡞ࡻࡽ ฦ㛣㝰࡚ *70 ࡡࣛࣤ㓗ࢅ⮤ິⓏ࡞ゝῼࡌࡾࡆ࡛࡞ᠺຉࡊࡒࠊ ERK ⤊㊪ࡡ⤣ゝ࣓ࢸࣜᵋ⠇ AR ࣓ࢸࣜࡡ⣌าࢸ࣭ࢰࡡ⤣ゝⓏ࣓ࢸࣜ࡞ࡗ࠷࡙࣓ࢸࣜࡡ᪁Ἢࢅ᳠ゞ ࡊࠉࣜࢥࣛࢫ࣑ࡡ㛜Ⓠࢅ㛜ጙࡊࡒࠊࡱࡍࡢ ERK ࡡ࢙ࣤࢤ࣭ࢺ᚜ฦ᪁⛤ᘟ࣓ࢸ ࣜࡡ⣌าࢸ࣭ࢰ࠾ࡼ AR ࣓ࢸࣜࢅషᠺࡌࡾᡥἪࢅ㛜Ⓠ୯࡚࠵ࡾࠊ ࣤࢪࣛࣤష⏕ࡡ⣌าࢸ࣭ࢰཱིᚋ ᇰ㣬⫚⣵⬂ࢅ⏕࠷࡙ࠉ࣓ࢸࣜᵋ⠇ࡡࡒࡴࡡࢨࢡࢻࣜఎ㐡⤊㊪ࡷ㐿ఎᏄⓆ⌟ࡡ ⣌าࢸ࣭ࢰࡷⓆ⌟㔖ࢸ࣭ࢰཱིᚋࢅ㛜ጙࡌࡾࠊࡱࡍࡢࣤࢪࣛࣤࡡࢪࢷࢴࣈๆ ⃥࡞ᑊࡊ࡙ࠉ5)05.NLQFVH ⤊㊪ࡡࢨࢡࢻࣜఎ㐡⤊㊪ࡷࠉࡐࡡୖὮࡡㅨ 㛭㏻㐿ఎᏄⓆ⌟ࡡ⣌าࢸ࣭ࢰࢅཱིᚋࡊࡒࠊࡐࡡ⤎ᯕࠉ⢶ㅨ㛭㏻㐿ఎᏄ⩄࡛ ⬙㈻ㅨ㐿ఎᏄ⩄ࡡ࠹ࡔୌ㒂ࡢࠉྜྷࡋࣤࢪࣛࣤๆ⃥࡚ࡵ␏ࡾ㛣ἴᙟࢅ♟ ࡌࡆ࡛ふῼࡈࡿࡒࠊࡱࡒࠉᑑᮮⓏ࡞ࡢ⫚⮒࡚ࡡᚺ➽ࢅ᳠ッࡌࡾࠉࡐࡡ๑ṹ 㝭࡛ࡊ࡙ୌḗᇰ㣬⣌⫚⣵⬂ࡷ +FR ⣵⬂ࡡ々ᩐࡡ⣵⬂ࢅ⏕࠷࡙ࢨࢡࢻࣜฦᏄ ࡷ㐿ఎᏄⓆ⌟භ㏳ࡡ㛣ᚺ➽ࢅ♟ࡌ࠾࠹࠾᳠ゞ୯࡚࠵ࡾࠊ 㸮㸣㸦㸣㸧 73&LQWHUIHUHQHHࢅ⏕࠷ࡒ⥑⨮Ⓩ㐿ఎᏄᶭ⬗よᯊ ࣃࢹࢅࡢࡋࡴ࡛ࡌࡾᵕࠍဳ㢦ࢣࢿ࣑ࣈࣞࢩ࢘ࢠࢹࡡ㐅⾔࡞ࡻࡽဳ㢦ࡡ㐿 ఎሒࢅᢰ࠹࠽࠽ࡻࡐࡡ㐿ఎᏄ≁ᏽࡈࡿࠉࢣࢿ࣑◂✪ࡢࠉࡐࡡᵋ㏸よᯊ࠾ࡼ ᶭ⬗よᯊ࡞Ⅴ⛛ࡖ࡙ࡀࡒ࡛ゕ࠻ࡾࠊ RNA interference (siRNA) ࡢࠉ࠵ࡾ㐿ఎᏄࡡ≁ᏽ㡷ᇡ࡛┞ྜྷ㸧ᮇ㙈 RNA (double-stranded RNA, dsRNA) ࠉ㌹ࡈࡿࡒ mRNA ࡡ┞ྜྷ㒂ฦࢅว᩷ࡌࡾ࡛࠷ ࠹⌟㇗࡚࠵ࡽࠉ1998 ᖳ࡞⥲࡚ጙࡴ࡙ሒ࿈ࡈࡿࡒࠊဳ㢦⣵⬂࡚ࡢࠉ⣑ 30 ሲᇱ ᑊ௧୕ࡡ㛏࠷ dsRNA ⣵⬂හථࡾ࡛ࠉࣜࢪ࡞ᑊࡌࡾ⏍మ㜭ᚒᶭᵋ࡚࠵ࡾ ࣤࢰ࣭ࣆ࢘ࣞࣤᚺ➽ㄇᑙࡈࡿࠉ⣵⬂Ṓࢆ࡚ࡊࡱ࠹ࡒࡴࠉ㛏࠷ dsRNA ࢅฺ ⏕ࡌࡾࡆ࡛ࡢࡳࡍ࠾ࡊ࠷ࠊࡊ࠾ࡊࠉ2001 ᖳ࡞ࠉ㛏࠷ dsRNA ࡢ Dicer ࡛࠷࠹ RNase 25 Ϫ㓕⣪࡞ࡻࡖ࡙ࠉ3’ 2 ሲᇱ✲ฝࡊࡒ▯࠷㸧ᮇ㙈 RNA(short interfering RNA, siRNA)࡞ว᩷ࡈࡿࡾࡆ࡛♟ࡈࡿࡒࠊࡐࡊ࡙ࠉᏕⓏ࡞ெᕝྙᠺࡊࡒ 21ࠤ23 ሲ ᇱᑊࡡ▯࠷ siRNA ࡞ࡻࡖ࡙ࡵࠉࣤࢰ࣭ࣆ࢘ࣞࣤᚺ➽ࢅ㉫ࡆࡈࡍ࡞ࠉRNAi ㄇ ᑙ࡚ࡀࡾࡆ࡛♟ࡈࡿࡒࠊဳ㢦⣵⬂࡚ RNAi ᐁ㥺ࢅ⾔࠹ሔྙࠉࡆࡡࡻ࠹Ꮥ ྙᠺࡊࡒ siRNA ࡱࡒࡢ siRNA Ⓠ⌟࣊ࢠࢰ࣭ࢅ⣵⬂ࢹࣚࣤࢪࣆ࢘ࢠࢨࣘࣤࡌࡾ ࡆ࡛࡞ࡻࡖ࡙ࠉRNAi ࢅㄇᑙࡌࡾ᪁Ἢ⏕࠷ࡼࡿࡾࠊRNAi Ἢࡢ⠾౼࡚ࠉ▯㛣 ࡚┘Ⓩ㐿ఎᏄࡡᶭ⬗ࢅᢒโ࡚ࡀࡾࠊࡊ࠾ࡵࠉሲᇱ㒼าࡢ᭩㸦㸮ሲᇱᑊࢂ࠾ࡖ ࡙࠷ࡿࡣࡻ࠷ࡒࡴࠉ࣎ࢪࢹࢣࢿ࣑ࢨ࣭ࢠ࢙ࣤࢪ࡞㐲ࡊࡒ᭯⏕㐿ఎᏄᶭ⬗ よᯊἪ࡛ࡊ࡙Ἰ┘ࡈࡿ࡙࠷ࡾࠊ ᮇ◂✪࡚ࡢࠉRNAi ࡞ࡻࡾ㐿ఎᏄᢒโᶭᵋࡡよ᪺࡛ࠉRNAi ࡞ࡻࡾ㐿ఎᏄᶭ⬗ よ᪺ࡡࡒࡴࡡᇱ┑Ⓩᢇ⾙ࡡ☔❟࡛ࠉࡐࡿࢅ⏕࠷ࡒ⥑⨮Ⓩࢪࢠ࣭ࣛࢼࣤࢡࢅ⾔ࡖ ࡙࠷ࡾࠊ RNAi ຝᯕࡡ㧏࠷ siRNA 㒼าࡡ㐽ᢝἪ ᠻࠍࡢࠉ࣌ࢰࣜࣜࢨࣆ࣭࢘ࣚࢭ㐿ఎᏄࠉဳ㢦හᅹᛮ㐿ఎᏄ࡞ᑊࡌࡾ RNAi ᐁ㥺ࠉ ࠽ࡻࡦమ࡞࠽ࡄࡾ RNAi ࡷ DNA ࣊ࢠࢰ࣭ᆵ RNAi ࡡ⛸ࠍࡡ RNAi ᐁ㥺ࢅ ⾔࠷ࠉຝࡂ siRNA ࡡ㒼าࡢࠉ௧ୖࡡ㸩ࡗࡡ᮪௲ࢅࡌ࡙⤎࡛ࡾ࠵࡚ࡡࡵࡌࡒ ㄵࡊࡒࠊ (1)࢝ࢺ㙈ࡡ 5’ᮆ❻ A ࡱࡒࡢ U ࡚࠵ࡾࠊ (2)ࣂࢴࢬࣤࢩ࣭ࣔ㙈ࡡ 5’ᮆ❻ G ࡱࡒࡢ C ࡚࠵ࡾࠊ (3)ࣤࢲࢬࣤࢪ㙈ࡡ 5’㡷ᇡ࡞ A ࡱࡒࡢ U ኣ࠷ࠊ (4)㛏࠷ GC ࡡ㏻⤾㒼า࠷ࠊ ࡆࡡࡻ࠹ siRNA ࡢࠉࡏ RNAi ຝᯕ㧏࠷ࡡ࡚࠵ࢀ࠹࠾ࠊRNAi ࡡᐁ⾔㐛⛤࡞ ࠽࠷࡙ࠉsiRNA ࡢ RNA-induced complex (RISC)࡞ཱིࡽ㎲ࡱࡿࡒᚃ࡞ࠉ㸧ᮇ㙈࠾ࡼ 㸦ᮇ㙈ࡡ unwinding ㉫ࡆࡾ࡛⩻࠻ࡼࡿ࡙࠷ࡾࠊ㸧ᮇ㙈 DNA ࡱࡒࡢ RNA ࡡ A ࡱࡒࡢ U ኣ࠷㡷ᇡࡢࠉG ࡱࡒࡢ C ኣ࠷㡷ᇡ࡞ẒᏭᏽ࡚࠵ࡽࠉ㸦ᮇ㙈 ࡄࡷࡌ࠷ࠊຝࡂ siRNA ࡡ ࢝ࢺ㙈ࡡ 5’㡷ᇡ࡞ A ࡱࡒࡢ U ኣ࠷࡛࠷࠹ࡆ ࡛ࡢࠉRNAi ࡡᐁ⾔㐛⛤࡞࠽࠷࡙ࠉ࢝ࢺ㙈ࡡ 5’ഁ࠾ࡼ㸦ᮇ㙈ࡄࡾࡆ࡛࡞ ࡻࡖ࡙ࠉRNAi ㉫ࡆࡽࡷࡌ࠷࡛࠷࠹ࡆ࡛ࢅ♟ြࡊ࡙࠷ࡾ࠾ࡵࡊࡿ࠷ࠊࡈࡼ࡞ࠉ ≁࡞࢝ࢺ㙈࠽ࡻࡦࣂࢴࢬࣤࢩ࣭ࣔ㙈ࡡ 5’ᮆ❻ࡡ㸦ሲᇱ㔔さ࡚࠵ࡾ࡛࠷࠹ࡆ ࡛࠾ࡼࠉ㸦ᮇ㙈ࡡ unwinding ௧አࡡืࡡᶭᵋࡵ㛭ࡊ࡙࠷ࡾࡡ࠾ࡵࡊࡿ࠷ࠊ ࡈࡼ࡞ࠉRNAi ᐁ㥺ࢅ⾔࠹࡞ࡢࠉoff-target ຝᯕ࡛࠷࠹ࠉᵾⓏ࡛ࡊ࠷㐿ఎᏄ 26 ࢅࡵᢒโࡊ࡙ࡊࡱ࠹࡛࠷࠹ࠉ࠷ࢂࡣష⏕࡛ࡵゕ࠻ࡾຝᯕࢅ⩻៎ࡌࡾᚪさ࠵ ࡾࠊOff-Target ຝᯕࡢࠉsiRNA ᵾⓏ࡛ࡌࡾ㐿ఎᏄ࡛㒼า୕ࠉᏰධ࡞ୌ⮬ࡊࡒሔ ྙࠉࡱࡒࡢᏰධ࡞ୌ⮬ࡊࡂ࡙ࡵ㟸ᖏ࡞జ࡙࠷ࡾሔྙ࡞ㄇᑙࡈࡿࡾࠊᠻࠍࡢࠉ ୕㏑ࡡຝࡂ㒼าࡡ᮪௲ࢅࠉࡊࡒᑛࡂ࡛ࡵ㟸ᵾⓏ㐿ఎᏄ࡛ࡢ᭩㸧ሲᇱ௧୕ ࡡ࣐ࢪ࣏ࢴࢲࡡ࠵ࡾ siRNA 㒼าࢅ㧏㏷࡚ῼ࡚ࡀࡾ࢙ࣇࢦࢹ㸝siDirect㸞ࡵ ᵋ⠇ࡊࠉප㛜ࡊ࡙࠷ࡾࠊ᭞࡞ࠉῼࡈࡿࡒ siRNA 㒼าࠉᵾⓏ㒼าࢅᐁ㝷࡞ࡢ ◒ቪࡌࡾࡆ࡛ࢅᐁ㥺Ⓩ࡞᳠ッࡌࡾࢨࢪࢷ࣑ࡵᵋ⠇࡚ࡀࡒࠊ 㛏࠷㸧ᮇ㙈 RNA ࡢ Dicer ࡞ࡻࡖ࡙ࠉ21bp ࡱࡒࡢ 22bp ࡞ว᩷ࡈࡿࡾ RNaseϪ 㓕⣪ࡡ㸦⛸࡚࠵ࡾ Dicer ࡢࠉRNA ᐁ⾔㐛⛤࡞࠽࠷࡙ࠉᑛࡂ࡛ࡵ㛏࠷ 㸧ᮇ㙈 RNA ࢅว᩷ࡊ࡙ siRNA ࢅషࡾࡆ࡛ࢂ࠾ࡖ࡙࠷ࡾࠊᠻࠍࡢࠉDicer ࡞ࡻ ࡾ㸧ᮇ㙈 RNA ࡡว᩷ࣂࢰ࣭ࣤࢅ in vitro ࡚よᯊࡌࡾᐁ㥺ࢅ⾔ࡖࡒࠊࡐࡡ⤎ᯕࠉ 㛏࠷㸧ᮇ㙈 RNA ࡢࠉDicer ࡞ࡻࡖ࡙ࠉ➠ୌṹ㝭࡚ࡢࠉ21, 22, ࡱࡒࡢ 23bp ࡡ siRNA ࡞ว᩷ࡈࡿࠉ᭩⤂Ⓩ࡞ࡢ 21 ࡱࡒࡢ 22bp ࡡ siRNA ࡞ว᩷ࡈࡿࡾࡆ࡛ࢂ ࠾ࡖࡒࠊᠻࠍࡢࠉ21bp ࡡ siRNA ࡞㛭ࡊ࡙ࡢࠉRNAi ຝᯕࡡ㧏࠷ siRNA ࡡ㒼าぞ ์ᛮࢅᥞ၌ࡊ࡙࠷ࡒࠉࡆࡡ⤎ᯕࢅ㊻ࡱ࠻࡙ࠉ22bp ࡡ siRNA ࡞࠽ࡄࡾຝࡂ㒼า ぞ์ᛮࡡ᳠ゞࢅ⾔࠷ࠉ௧ୖࡡ᮪௲ࢅ ࡌࡒ22bp siRNA ࡢࠉ㟸ᖏ࡞㧏࠷☔⋙࡚᭯ ຝ࡚࠵ࡾࡆ࡛᪺ࡼ࠾࡛ࡖࡒࠊ (1)࢝ࢺ㙈ࡡ 5’ᮆ❻ A ࡱࡒࡢ U ࡚࠵ࡾࠊ (2)ࣂࢴࢬࣤࢩ࣭ࣔ㙈ࡡ 5’ᮆ❻ G ࡱࡒࡢ C ࡚࠵ࡽࠉࡐࡡ㝼ࡡሲᇱࡵ G ࡱࡒࡢ C ࡚࠵ࡾࠊ (3)ࣤࢲࢬࣤࢪ㙈ࡡ 5’㡷ᇡ࠾ࡼ㸬ሲᇱࡡ࠹ࡔ㸩ሲᇱ௧୕ࡢ A ࡱࡒࡢ U ࡚࠵ࡾࠊ (4)㛏࠷ GC ࡡ㏻⤾㒼า࠷ࠊ DNA ࣊ࢠࢰ࣭࡚ siRNA ࢅⓆ⌟ࡈࡎࡾ࡞ࡢࠉstem-loop ᆵࡡୌḗ㌹⏐∸ࡢࠉ Dicer ࡞ࡻࡖ࡙ siRNA ࡛ࡾࠊࡆࡡ࡞ࡵ 21bp ࡡࡲ࡚ࡢࡂ 22bp siRNA ࡵฝᮮ ࡾ࡛⩻࠻ࡼࡿࡾࡒࡴࠉᮇ◂✪࡞ࡻࡾ⤎ᯕࢅཱིࡽ㎲ࡳࡆ࡛࡞ࡻࡖ࡙ࠉࡻࡽᏰᠺᗐ ࡡ㧏࠷ siRNA Ⓠ⌟ࣚࣇࣚࣛࡡᵋ⠇ྊ⬗࡛ࡖࡒࠊ 㸧⛸ࡡ siRNA ࢅ├า࡞⦽࠷ࡓ㸧ᮇ㙈 RNA ࡞ࡻࡾ RNAi Off-target ຝᯕࡢࠉ々ᩐࡡ siRNA ࢅྜྷ࡞⏕ࡌࡾࡆ࡛࡞ࡻࡖ࡙ࠉ᭩ᑚ㝀࡛ࡌࡾ ࡆ࡛ྊ⬗࡛⩻࠻ࡼࡿࡾࠊࡌࢂࡔࠉᵾⓏ㐿ఎᏄࡡ␏ࡾ㒂న࡞ᑊࡌࡾ㸧⛸௧ ୕ࡡ siRNA ࢅྜྷ࡞⏕ࡊࡒሔྙࠉࡐࡿࡑࡿ siRNA ࡡ off-target ࡛ࡾ㟸ᵾⓏ㐿 27 ఎᏄ␏ࡖ࡙࠷ࡿࡣࠉoff-target ຝᯕࡢ᭩ᑛ࡛ࡽࠉᵾⓏ㐿ఎᏄࢅຝ⋙ࡻࡂᢒ โࡌࡾࡆ࡛ྊ⬗࡚࠵ࡾࠊࡐࡆ࡚ࠉ㸧⛸ࡡ siRNA ࢅ├า࡞⦽࠷ࡓ 43bp ⛤ᗐࡡ㛏 ࠷㸧ᮇ㙈 RNA ࡞ࡻࡾ RNAi ຝᯕࢅ᳠ゞࡊࡒࠊDicer ࡞ࡻࡾ in vitro ࡚ࡡ㸧ᮇ㙈 RNA ࡡฦよࡢࠉࡱࡍᮆ❻㒂ฦࢅヾㆉࡊ࡙ࠉᮆ❻㒂ฦ࠾ࡼ 21 ࡱࡒࡢ 22bp ࡡ㒂నࢅว ᩷ࡌࡾࠊࢨࣘࢩࣘࣁ࢙⣵⬂࠽ࡻࡦࢲࣔࢼ࣭ࢫࣀ࣑ࢪࢰ࣭⣵⬂࡚ࡢࠉ 㸧ᮇ㙈 RNA ࡡᮆ❻࠾ࡼ in frame ࡚᭯ຝ siRNA 㒼าᏋᅹࡊ࡙࠷ࡾ࡛ࠉ㛏࠷㸧 ᮇ㙈 RNA ࡚ࡵຝ⋙ࡻࡂ RNAi ㄇᑙ࡚ࡀࡾࡆ࡛ࢂ࠾ࡖࡒࠊout of frame ࡡሔྙ ࡞ࡢ RNAi ࡢㄇᑙ࡚ࡀ࠾ࡖࡒࡆ࡛࠾ࡼࠉ⣵⬂හ࡚ࡵࠉDicer ࡞ࡻࡾว᩷ࡢᮆ❻ 㒂ฦࢅヾㆉࡊ࡙㉫ࡆࡾࡆ࡛ࢂ࠾ࡽࠉin frame ࡡ㛏࠷㸧ᮇ㙈 RNA ࢅ⏕࠷ࡾࡆ࡛ ࡞ࡻࡖ࡙ࠉࢱࣇࣜࢿࢴࢠࢱ࡚ࣤࡀࡾࡆ࡛ࢂ࠾ࡖࡒࠊ siRNA ࣚࣇࣚࣛࡡᵋ⠇࡛ࢪࢠ࣭ࣛࢼࣤࢡ ࣃࢹධ㐿ఎᏄᩐࡢࠉ࠽ࡻࡐ ⛤ᗐ࡚࠵ࡾࡆ࡛ࠉࣃࢹࢣࢿ࣑ࣈࣞࢩ࢘ࢠࢹ ࡡ⤎ᯕࠉ᪺ࡼ࠾࡞ࡈࡿ࡙ࡀࡒࠊᩝ㒂⛁Ꮥ┤ࢣࢿ࣑ࢾࢴࢹ࣭࣠ࢠࣈࣞࢩ࢘ࢠࢹࡡ ୌ⎌࡛ࡊ࡙ࠉᖲᠺ ᖳᗐࡱ࡚࡞ࠉࡆࡿࡼࡡ࠽ࡻࡐ 㸚࡞┞ᙔࡌࡾ⣑ 㐿 ఎᏄ࡞ᑊࡌࡾ VL73& ࣚࣇ ࣚࣛࢅᵋ⠇ࡊࡒࠊࡆࡿ ࡼࡡᵾ Ⓩ㐿ఎ Ꮔࡢ ,HQH 4QWRORJ^ ࡞ࡻࡖ࡙ฦ㢦ࡊࠉ㌹ᅄᏄࠉࢰࣤࣂࢠ㈻ࣛࣤ㓗㓕⣪ඁᗐࡡ㧏 ࠷ࡵࡡ࠾ࡼ㡨ḗ VL73& Ⓠ⌟ࢠ࣭ࣞࣤࢅష⿿ࡊ࡙࠷ࡖࡒࠊᵋ⠇ࡊࡒධ࡙ࡡࢤࣤࢪ ࢹࣚࢠࢹࡡሲᇱ㒼าࡢࢨ࣭ࢠ࢙ࣤࢪ࡞ࡻࡖ࡙㛣㐢࠷࠷ࡆ࡛ࢅ☔ヾࡊࡒࠊష ⿿ࡊࡒࢤࣤࢪࢹࣚࢠࢹࡢࠉ㸦㸞ࡐࡡࡱࡱࣈࣚࢪ࣐ࢺ࡛ࡊ࡙ࢹࣚࣤࢪࣆ࢘ࢠࢨࣘ ࣤࡊ࡙⏕ࡌࡾࡆ࡛ࡵ࡚ࡀࡾࡊࠉ㸧㸞ࣝࢹࣞࣜࢪ࡛ࡊ࡙⏕ࡌࡾࡆ࡛ࡵ࡚ ࡀࡾࠊࣝࢹࣞࣜࢪ࡛ࡊ࡙⏕ࡌࡾሔྙ࡞ࡢࠉష⿿ࢤࣤࢪࢹࣚࢠࢹࢅࣂࢴࢢ ࣭ࢩࣤࢡ⣵⬂㸝ࣝࢹࣞࣜࢪ⏐⏍⣵⬂㸞࡞ࢹࣚࣤࢪࣆ࢘ࢠࢨࣘࣤࡊ࡙ᇰ㣬୕ ΰ࡞⏐⏍ࡈࡿࡾࣝࢹࣞࣜࢪࢅ┘Ⓩࡡ⣵⬂࡞វࡈࡎࡿࡣࡻ࠷ࠊ ࡈࡼ࡞㎾ᖳࠉ*8 ⣵⬂ࡷ♼⤊⣵⬂࡞ࡵ㐲⏕ྊ⬗ࣝࣤࢲࣜࢪᆵ࣊ࢠࢰ࣭ࡡ ᨭⰃ㐅ࡲࠉⰃይ 73&L ຝᯕᚋࡼࡿࡾࡆ࡛᪺ࡼ࠾࡞ࡖ࡙ࡀࡒࠊࡐࡆ࡚ࣝ ࢹࣞࣜࢪᆵ࡞ຊ࠻࡙ࣝࣤࢲࣜࢪᆵࢤࣤࢪࢹࣚࢠࢹࡡష⿿ࢅ⾔࠷⣑ 㐿ఎᏄ㸝࡞㌹㛭㏻㐿ఎᏄ㸞࡞ᑊࡌࡾࢤࣤࢪࢹࣚࢠࢹࢅᏰᠺࡊࡒࠊࡱࡒࠉ ⣑ 㐿ఎᏄ㸝࡞㌹㛭㏻㐿ఎᏄࠉ࣐ࢹࢤࣤࢺࣛ㛭㏻㐿ఎᏄ㸞࡞ᑊࡌࡾ 73&SRO^PHUFVH..SURPRWHU ᆵࡡࢤࣤࢪࢹࣚࢠࢹࡵᏰᠺࡊࡒࠊ⣑ 㐿ఎᏄ ࡞ࡗ࠷࡙ࡢࣃࢹ㸤࣏ࢪභ㏳㒼าࢅ⏕࠷࡙࠷ࡾࠊ ࣃࢹࠉ࣏ࢪࡡ㌹ᅄᏄࠉ࣎ࢹ࣭ࢨࢪ㛭㏻㐿ఎᏄࠉ࣐ࢹࢤࣤࢺࣛ㐿ఎᏄࠉ 28 ᰶ㓗⤎ྙ㐿ఎᏄࡡࣚࣇࣚࣛࢅฺ⏕ࡊ࡙ࠉ࣏ࢪ Embryonic stem (ES)⣵⬂ࠉ ࣃࢹ HeLa ⣵⬂࡚ࡡ㐿ఎᏄᶭ⬗ࢅㄢࡒࠊࡐࡡ⤎ᯕࠉㄢࡒ㌹โᚒᅄᏄࡡ⣑༖ ฦ ES ⣵⬂ࡡฦ࠵ࡾ࠷ࡢᙟឺࡡỬᏽ࡞㛭ࢂࡖ࡙࠷ࡾࡆ࡛ࠉኣᩐࡡ࣐ࢹࢤࣤࢺ ࣛ㐿ఎᏄ࣎ࢹ࣭ࢨࢪࡡㄇⓆ࡞㛭ࢂࡖ࡙࠷ࡾࡆ࡛ࠉࡈࡼ࡞ኣᩐࡡ㐿ఎᏄ RNAi ຝᯕ࡞ᙫ㡢ࢅ࠻ࡾࡆ࡛ࢂ࠾ࡖ࡙ࡀࡒࠊ⌟ᅹࠉࡆࡿࡼࡡよᯊࢅレ⣵࡞⾔ ࡖ࡙࠷ࡾࠊ 㸮㸣㸧 ◂✪ᠺᯕ 㸱Ꮥఌヽ➴㸳 (1) Maeda, A., Ozaki, Y., Sivakumaran, S., Akiyama, T., Urakubo, H., Usami, A., Sato, M., Kaibuchi, K. and Kuroda, S. (2006). Ca2+-independent phospholipase A2-dependent sustained Rho-kinase activation exhibits all-or-none response. Genes Cells, 11, 1071-1083 (2) Fujioka, A., Terai, K., Itoh, R.E., Aoki, K., Nakamura, T., Kuroda, S., Nishida, E. and Matsuda, M. (2006). Dynamics of the Ras/ERK MAP kinase cascade as monitored by fluorescence probes. J. Biol. Chem., 281, 8917-8926 (3) Ui-Tei, K., Naito, Y. and Saigo, K. (2006). Essential notes regarding the design of functional siRNAs for efficient mammalian RNAi. J. Biomed. Biotechnol. 2006(4), 14417-14422. (4) Kamiyama, S., Sasaki, N., Goda, E., Ui-Tei, K., Saigo, K., Narimatsu, H., Jigami, Y., Kannagi, R., Irimura, T. and Nishihara, S. (2006). Molecular cloning and characterization of a novel 3’-phosphoadenosin 5’-phosphosulfate transporter, PAPST2. J. Biol. Chem. 281, 10945-10953. (5) Naito, Y., Ui-Tei, K., Nishikawa, T., Takebe, Y. and Saigo, K. (2006). siVirus: web-based antiviral siRNA design software for highly divergent viral sequences. Nucleic Acids Res. 34, W448-450. (6) Ui-Tei, K., Naito, Y. and Saigo, K. (2007). Guidelines for the selection of functional siRNA sequences for mammalian RNAi. Methods Mol. Biol. 361, 201-216. (7) Kojima, S., Matsumoto, K., Hirose, M., Shimada, M., Nagano, M., Shigeyoshi, Y., Hoshino, S., Ui-Tei, K., Saigo, K., Green, C.B., Sakaki, Y. and Tei, H. (2007). LARK activates posttranscriptional expression of an essential mammalian clock protein, PERIOD1. Proc. Natl. Acad. Sci., USA. 104, 1859-1864. 29 (8) Ui-Tei, K., Zenno, S. and Saigo, K. Sequence requirements for effective mammalian RNAi induced by short double-stranded RNAs equal to or more than 22bp in length. In; New Research on Antisense Elements (Genetics). Nova Science Publishers, Inc. In press. 㸱ᩝ⥪ㄕ㸳 (1) ⛤⨶Ꮔ㸣࣎ࢪࢹࢣࢿ࣑ࢨ࣭ࢠ࢙ࣤࢪࡡ㐿ఎᏄᶭ⬗よᯊἪ-RNAi㸣ࣁ ࢛ࢷࢠࢿࣞࢩ࣭ࢩ࣭ࣔࢻࣜ㸣6, 20-22, 2006. (2) හ⸠㞕ᶖࠉᒜ⏛ᬓࠉ⛤⨶Ꮔࠉୖ┷ୌࠉこ㒋ⷽ㸣siRNA 㐽ᢝἪ -㒼า ࢅ㐽ᢝࡌࡾ࠾ࠉຝࡄࡣⰃ࠷࠾㸣ࣁ࢛ࢷࢠࢿࣞࢩ࣭ࢩ࣭ࣔࢻࣜ㸣6, 23-28, 2006. (3) ⛤⨶Ꮔࠉこ㒋ⷽ㸣ᇰ㣬⣵⬂ࡡࢹࣚࣤࢪࣆ࢘ࢠࢨࣘࣤ࡞ࡻࡾ RNAi㸣ࣁ ࢛ࢷࢠࢿࣞࢩ࣭ࢩ࣭ࣔࢻࣜ㸣6, 29-33, 2006. (4) ⛤⨶Ꮔࠉこ㒋ⷽ㸣㐿ఎᏄⓆ⌟᧧ష I -⣵⬂-㸣㐿ఎᏄᕝᏕ㞗୯࣏ࢪࢰ࣭㸣 2006. (5) ⛤⨶Ꮔࠉᱼᾀᙢ㸣RNAi ᐁ㥺ࡾ Q&A. ௺⏤࣬⥽㞗㸣⨲ᅰ♣㸣2006. (6) ᮙᮇல▹Ꮔࠉ⛤⨶Ꮔ㸣RNA ᖱῦ㸯㸧ᮇ㙈 RNA ࡞ࡻࡾ᩺ࡊ࠷㐿ఎᏄᶭ⬗ โᚒᶭᵋࡡⓆぜ㸣⛁Ꮥ㸣77, 13-15, 2007. 30
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