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Microbial Genomic DNA

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RIKEN BioResource Center Gene Engineering Division
RIKEN DNA Bank catalog #
Microbial Genomic DNA
T
Strain: JCM 7501
Organism: Thermoanaerobacter thermocopriae
JGD
Lot # :
12533
12533_A3K8
Date :
2015/11/19
1. Shipping
Volume: 30 ul
2. Concentration of Genomic DNA
45.7
1.9
Concentration (OD260):
A260/A280:
ng/ul
3. Electrophoresis of Genomic DNA
Electrophoresis : 1% agarose gel, 1x TAE buffer
Loading DNA amount : 4, 2, 1 ul/lane
Size marker : Lambda/HindIII (200 ng/lane)
2
1
Marker
Lambda/HindIII
(NEB#N3012S)
0.5 (ul)
23.1
6.6
9.4
4.4
2.0
2.3
(Kb)
0.6
4. PCR Amplification
2015/11/19
Date :
Conditions of PCR ribosomal RNA (rRNA) gene
Template : 100 fold diluted DNA, 1 ul
Enzyme : KOD Plus (Toyobo)
Volume : 20 ul
Reaction program: 94
94
x30 cycle
60
68
68
degC 2 min
degC 15 sec
degC 30 sec
degC 60 sec
degC 2 min
PCR primers
16S rRNA Eubacteria set
EB-20F
5' AGTTTGATCCTGGCTC 3'
EB-350F 5' TACGGGAGGCAGCAG 3'
EB-1100R 5' AGGGTTGCGCTCGTTG 3'
EB-1400R 5' ACGGGCGGTGTGTAC 3'
EB-1530R 5' AAGGAGGTGATCCAGCC 3'
(1) (2) (3) (4) (5) (6)
Sample Number
EB-1100R
EB-1400R
EB-1530R
EB-20F
(1)
(2)
(3)
EB-350F
(4)
(5)
(6)
Electrophoresis : 1% agarose gel, 1x TAE buffer
Loading DNA amount :
3
ul PCR product/lane
Size marker : Lambda/HindIII (200 ng/lane)
5. Nucleotide sequence of rRNA gene
□Confirmed date :
2015/11/27
http://dna.brc.riken.jp
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