他社SPEとの比較データ

d_GLC04_01(p001-044).qxd 04.9.1 4:51 PM ページ 7
R
R
STRATA™
固相抽出
Solid Phase Extraction
n
STRATATM SPE 製品選択ガイド
STRATATM チューブは超高純度の医療用ポリプロピレンを原材料として使用しています。業界標準サイズですので
真空マニホールドや自動化システムにそのままご利用いただけます。リザーバサイズは１，3，6mlです。
STRATA固定相はシリカ，ポリマーの2種類の基材に無極性，強極性，そしてイオン相と幅広いレンジをもっています。
充てん容量は50∼1000mgです。
【STRATA C18-M vs. W社 C18 SPE】
【STRATA C18-E vs. V社 C18 SPE】
mAU
2
mAU
1
4
2
20
3
I.S.
5
Standard
5
6
7
15
4
3
3
1
4
2
5
6
Standard
9
7 8
10
11
-10
10
11
1
13
12
-20
14
0
-30
0
10
20
30
40
50
5
20
3
Phenomenex
Strata
2
2
4
3
1
6 7 8 9
10
15
20
25
30
mAU
5
4
10
35
min
min
I.S.
mAU
1
9
8
0
Phenomenex
Strata
2
1
4
5
15
6
3
8
7
0
9
11
10
-10
11
5
13
12
0
14
-20
-30
10
20
30
40
50
min
0
5
10
15
20
25
30
35
min
I.S.
mAU
mAU
5
1
2
20
4
2
2
1
6
4
1
5
3
9
7
5
15
6
3
11
8
7
0
10
8
V社 C18 SPE
4
W社 C18 SPE
3
11
9 10
-10
12 13 14
0
-20
10
20
30
40
50
min
-30
0
5
10
■Catecholamines
■Sulfa Drugs
SPE CONDITIONS
SPE CONDITIONS
Tube Size:
Sample:
Tube Size:
Sample:
Condition:
Wash:
Dry:
Elution:
Dilution:
C18-M 500mg/ 3mL
1mL urine + 0.1M Ammonium chloride containing 0.2% diphenyl borinic acid
ethanolamine complex
1mL Methanol followed by 1mL 0.1M Ammonium chloride containing 0.2%
diphenyl borinic acid ethanolamine complex,pH 8.5
2mL DI water
2.5mL 30:70 Methanol/0.1N HCl
Condition:
Wash:
Elution:
HPLC CONDITIONS
Column:
Mobile Phase:
Luna 5μm C18(2), 150 x 4.6mm
Methanol/50mM KH2PO4 (containing 80mg/L sodium octane sulfonic acid) pH
2.0 (5:95)
UV@215nm
0.65mL/min from start to 20min, then 1 min gradient to 1.5mL/min; hold for
9min and finally, 1 min. gradient to 2.0mL/min and hold till the end of the run
56 min
1. Norepinephrine
8. Dopac
2. VMA 9. Dopamine
9. Dopamine
3. 4-Hydroxy-3-Methoxy10. L-DOPA
phenylglycol (Mopeg)
11. Deoxyepinephrine
4. Epinephrine
12. HVA
5. 5-OH Dopamine
13. Serotonin
6. DHBA
14. 5-OH Tryptophan
7. Normetanephrine
I.S.=Isoproterenol
Detection:
Flow Rate:
Run Time:
Sample:
【STRATA C18-E vs. S社 C18 SPE】
15
20
25
30
35
min
C18-E 200mg/3mL
2mL porcine serum + 400μL Std mix + 2mL 100mM NaOAc pH 5
1mL Methanol, 1mL 100mM NaOAc pH 5
1mL 100mM NaOAc pH 5
5 minutes under vacuum
1mL 9:1 Methanol/100mM NaOAc, pH 5
14mL XS solution (128μL 1mg/mL sulfanilamide
+ 344μL 0.5 mg/mLsulfacetamide + 200mL water)
HPLC CONDITIONS
Column:
Mobile Phase:
Gradient:
Detection:
Flow rate:
Sample:
Luna 3μm C18(2), 100 x 4.6mm
A = Acetonitrile; B = 25mM KH2PO4
hold at 2% A for 5 minutes, 2% to 30% A over 30 minutes, hold at 30% A for 5
minutes, 30% to 2% A over 5 minutes
UV@254nm
1 mL/min
1. Sulfanilamide (XS1)
7. Sulfabenzamide
2. Sulfacetamide (XS2)
8. Sulfamethoxazole
3. Sulfadiazine 9. Sulfasalazine
4. Sulfathiazole
10. Sulfadimethoxine
5. Sulfamerazine
11. Sulfinpyrazone
6. Sulfamethizole
【STRATA C18-E vs. M社 C18 SPE】
mAU
mAU
1
50
60
40
2
40
6
4
3
5
30
Standard
7
20
Standard
20
8
10
0
0
0
0
5
10
15
20
5
10
15
20
25
30
min
25 min
mAU
1
mAU
60
2
40
4
3
5
6
50
Phenomenex
Strata
7
20
40
30
Phenomenex
Strata
20
8
10
0
0
0
mAU
5
10
15
20
25 min
0
1
60
10
15
20
25
30
min
50
S社 C18 SPE
2
40
5
mAU
4
3
5
40
6
M社 C18 SPE
30
7
20
8
20
0
10
0
5
10
15
20
0
25 min
0
■Analgesics
■TCA
SPE CONDITIONS
SPE CONDITIONS
Tube Size:
Sample:
Condition:
Wash:
Elution:
Tube Size:
Sample:
Condition:
Wash:
Dry:
Elution:
C18-E 500mg/ 3mL
1mL porcine serum acidified with 20μL H3PO4
2 x 2mL Methanol, 2 x 2mL Water
2mL Methanol/Water (3:7)
3 x 500μL Methanol/Acetonitrile/1N HCl (7:2:1)
HPLC CONDITIONS
Column:
Mobile Phase:
Detection:
Flow Rate:
Sample:
Luna 5μm C8(2), 75 x 4.6mm
20mM Na2HPO4 at pH 6/Acetonitrile (75:25)
UV@202nm
0.75mL/min
1. Indoprofen
5. Diflunisal
2. Tolmetin
6. Flurbiprofen
3. Naproxen
7. Ibuprofen
4. Fenoprofen 8. Indomethacin
SHIMADZU GLC LTD.
5
10
15
20
25
30
min
C18-E 100mg/ 1mL
1mL spiked porcine serum
2 col vol Methanol, 2 col vol Water
1 col vol 22.5:77.5 Methanol/ 0.1N Acetic Acid
30sec under vacuum
600μL Methanol/Acetonitrile/1N HCl (4:4:2)
HPLC CONDITIONS
Column:
Mobile Phase:
Detection:
Flow rate:
Sample:
Luna 5μm C18(2), 150 x 4.6mm
Methanol/Acetonitrile/20mM Phosphate Buffer, pH 7 (45.5:19.5:35)
UV@254 nm
0.75 mL/min
1. Propylparaben
7. Doxepin
2. Butylparaben
8. Imipramine
3. Nordoxepin
9. Amitriptyline
4. Protriptyline
10. Trimipramine
5. Desipramine
11. Clomipramine
6. Nortriptyline
URL : https://solutions.shimadzu.co.jp/glc |
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