0. 1 M Kcl solution 0.25 N Potassium Dichromate

0. 1 M Kcl solution
7.4 gm of Kcl was dissolved in I litre of dist., water.
0.25 N Potassium Dichromate solution
12.25 gm of K 2Cr2 0 7 dried at 105°C for 2 hrs, was dissolved in 1 litre of dist.,
water.
0.1 M Ferrous Ammonium Sulphate solution
59.2 gm of FAS was dissolved in 500 ml of dist. water. 20 in! of Sulphuric
acid was added to it and it was diluted to 1000 ml using dist., water.
Potassium Hydrogen Phthalate
425 mg of KHP was dissolved in 1000 ml of dist. water
Ferroin indicator
1.485 gin of 1,10 Phenanthroline Monohydrate and 695 mg of Ferrous
Sulphate (FeSO4 .7H70) were dissolved in 100 ml of dist., water.
Phosphate buffer
8.5 gin of KH 2PO4 , 21.75 gm of K 2HPO4 , 33.4 gin of Na 2HPO4 .7H20 and 1.7
gin of NH4 C1 was dissolved in about 500 ml of dist. water and diluted to 1 L.
Magnesium Sulphate
22.5 gm of MgSO4 .7H20 was dissolved in dist. water and was diluted to 1L.
Calcium Chloride
27.5 gin of CaC1 2 was dissolved in dist. water and diluted to 1L
Ferric Chloride
0.25 gin of FeCI 3 .61470 was dissolved in dist. water and diluted to 1L.
Manganese Sulphate
480 gin of tetrahydrate Manganese Sulphate was dissolved in dist. water
and diluted tol000iyìl.
Alkali Iodide Azide Solution
250 gin of NaOH 67.5 gin of Nal were dissolved in 500 ml and 5gm of
NaN 3 was dissolved in 20 ml of dist. water The two solution were mixed together
and diluted to 1000ml.
Starch solution
0.5 gin
starch powder was dissolved in boiling water and was allowed to
boil for few minutes The boiled solution was kept in a cold place.
Standard Sodium Thiosulphate solution (0.025N)
24.82 gin of Na 2 S0 3 .5H70 was dissolved in 1000 ml .250 ml of this solution
was dissolved in 1000inl dist. water: This solution was preserved adding 2 to 3 ml
of Chloroform.
Digestion reagent for estimation of total Nitrogen
134 gm of Potassium Sulphate in 650 ml of dist. water was dissolved in 200
ml of con. Sulphuric acid. 2 gm of Mercuric Oxide in 25 ml of 6N Sulfuric acid was
added to the above solution and it was diluted to 1000 ml using dist. water.
Sodium hydroxide-Sodium thiosuiphate solution
500 gm of NaOH and 25 gin of Na2S 2 0 3 were dissolved in 1000m! of dist.
water.
Mixed Indicator solution (for TKN)
200 mg of Methyl red in 100 ml of 95% Ethanol and 100 gm of Methylene
blue in 50 ml of 95% Ethanol were mixed together.
Boric Acid reagent
20 gm of Boric Acid was dissolved in 1000 ml of dist. water with addition of
10 ml of mixed indicator.
Sulfuric acid 0.02N
0.1N of sulfuric acid was prepared by diluting 3 ml of con., sulfuric acid to
1000 ml. 20 ml of this solution was further diluted to 100 ml for the preparation of
0.02N HSO4.
Sulfuric acid 5N
70 ml of con. sulfuric acid was diluted to 500 ml using dist., water.
Potassium antimonyl tartarate
1.372 gm of Potassium antirnonyl tartarate was dissolved in 400 ml of dist.
water and it was made up to 500 ml in a volumetric flask.
Ascorbic Acid
1.76 ml of Ascorbic acid was dissolved in 100 ml of dist. water
Ammonium Molybdate solution
20 gm of Ammonium molybdate was dissolved in 500 ml of dist. water.
Methyl Orange
0.5 ml of methyl orange was dissolved in 100 ml of dist. water.
0. IN Sodium Hydroxide
4 gm of Sodium Hydroxide was dissolved in 1000 ml of dist. water.
Potassium Chromate indicator solution
50 gm of Potassium chromate was dissolved in 1000ml of dist. water. Silver
nitrate solution 0.0141N was added to it till the red precipitate was formed. This
solution was allowed to stand for 12 hrs. and then it was filtered through, filter
paper. The filtrate solution was used as indicator.
Silver Nitrate solution 0.014N
2.395 gm of silver nitrate solution was dissolved in double dist. water and it
was diluted to 1000ml. This solution was standardized against 0.014N sodium
chloride.
Standard Sodium Chloride solution
824.1 mg of NaC1 dried at 140°C was dissolved in 1000 ml of dist. water.
Buffer solution
30 gin of magnesium chloride, 5 gm of sodium acetate, 1 gin of potassium
nitrate and 20 ml of acetic acid was dissolved in 500 ml of distilled water and
diluted to 1000 ml.
Siebert and Hattingh's medium (1967):
MPN of Methanogenic bacteria
KH7HPO4 .3H 20
KI-1 2PO46.8
17.4 g
g
NI-LOI
1.0 g
NaH CO3
2.0 g
Sodium formate
10.0 g
Cysteine hydrochloride
0.1 g
Sodium thioglycollate
0.1 g
Trace mineral solution (a)
1 ml
Vitamin solution (b)
1 ml
Distilled water to make
1C'OO ml
a) Trace mineral solution.
\lnSO4
500 mg
COO2
500 mg
Ferri:. citrate
500 mg
Cacl.6H7O
500 ing
N aMoO, . 2H2O
500 mg
CuSo1.5H7O
500 mg
Distilled water
100 ml
b) Vitamin solution
Aneurin hydrochloride
5 rng
L-Ascorbic acid
5 mg
Riboflavin
5 mg
Biotin
10 mg
Vitamin B12
5 mg
Pyridoxin hydrochloride
1.2 mg
Folic acid
2 ing
Distilled water
lOOml
Mahe et. cii. (1977)
NI-14C1
Medium:
1.0 g
Yeast extract 2.0 g
Tiypticase
2,0 g
KHPO4.3H70
0.4 g
i\'lgcl2.6H20
0.1 g
NaHCO3
0.5 g
Cysteirie hydrochloride
0.5 g
Na7S .9H20
0.1 g
Sodium acetate
or
\Iethinol
8.2 g
4.0 ml
Mineral solution (a)
50.0 nil
vitamin solution (b)
5.0 ml
Resazurin indicator (0.1 % w/v)
1.0 ml
Distilled water to make
1000 ml
Mineral solution
Mgcl2.6H20
40 mg
CaC17.2H20
53 mg
CoCI7.6H70
2 ing
MnC17.4H20
20 mg
Distilled water to make
1000 ml.
Vitamin solution
Thiamine hydrochloride L-Calcium pantothenate
22.0 mg
20.0 mg
Nicotinamide
Riboflavin•
20.0 mg
20.0 mg
Pyridoxin hydrochloride
Biotin
Folic acid
20.0 mg
0.50 rng
Vitamin B17
0.25 mg
Distilled water to make
0.20 mg
100 ml
1
ANOV.2AS[V.5.1] - ANOVA - TWO-WAY ANALYSIS
2
written by: Thomas Shairn Rajareegam
3
date of last modification: FEB-22-1982
4 ' computer system: IBM PERSONEL COMPUTER SYSTEM
5
language: MICROSOFT BASIC [v.Di.00]
6 ' COPYRIGHT 1982 BY:
7 ' BASIC BUSINESS SOFTWARE, INC.
8
POST OFFICE BOX 26311 / LAS VEGAS , NV. 89126
9
' (702) 876-9493
10 DEFDBL A-Z : DEFSNG I,J,K,Z : ON ERROR GOTO 61000
11 OPEN "I",#i,"PRINTER.DAT"
12 INPUT #i,SLOT$
13 CLOSE 41 : DIM R(500),C(500)
14 SLOT=VAL (SLOTs)
15 DEF FNS$(X)=RIGHT$(STR$(x),LEN(STR$(X))l)
16 ON ERROR GOTO 60000
17
100 KEY OFF : CLS
COLOR 0,7,0
101 PRINT"'STATISTICS' - A Statistical Analysis Package[v 5.11
Date: ;DATE$
105 PRINT"Program: Two-Way Analysis of a Variance
Time:
TIME$
110 PRINT"
(c) 1982
Basic Business Software, Inc.
115 COLOR 7,0,0
175 FOR I = 1 TO 500:R(I) = 04:C(I) = 044: NEXT I
176 Si = 044:52 = 044:59 = 044:R1 = 04:C1 = 044:C6 = 044
177 GOSUB 50000
178 IF LEFT$ ( A $, l ) = "Y" THEN 370
179 LOCATE 4,1 : GOSUB 63060
180 PRINT : PRINT : PRINT : PRINT : PRINT
190 PRINT "HOW MANY ROWS
INPUT R
200 PRINT "HOW MANY COLUMNS ";: INPUT C
205 Ni = R * C
210 CLS
220 COLOR 0,7,0 : PRINT "
'H COLOR 7,0,0
240 PRINT : PRINT : PRINT
250 FOR J = 1 TO R
260 FOR K = 1 TO C
270 PRINT "ENTER A VALUE FOR ROW";J;" COLUMN";K;
280 INPUT X
290 R(J) = R(J) + x
300 C(K) = C(K) + X
310 Si = X + Si
320 S2 = S2 + X ' 244
330 S9 = S9 + X
340 NEXT K
350 PRINT
360 NEXT J
370 CLS
380 COLOR 0,7,0 : PRINT " RESULTS
": COLOR 7,0,0
400 PRINT
405 Ri = 044:C1 = 04:C6 = 044
410 Ti = S2 - Si * 51 / (R * C)
420 FOR Z = 1 TO R:Ri = Ri + R(Z) * R(Z) / C: NEXT Z
"S:
DATA ENTRY
SUMMARY OF
430 FOR Z = 1 TO C:Ci = Cl + C(Z) * C(Z) / R: NEXT Z
440 Ri = Ri - Si * Si / (R * C)
450 FOR CS! = 1 TO C:C6 = C6 + C(C8!) * C(C8!) / R: NEXT C8!
460 Cl = C6 - (Si * Si / (R * C))
470 El = Ti - Ri - Cl
480 Di = R 490 D2 = C 500 E2 = (R
i) * (C - i#)
510 Fl = (Ri / Di) / (El / E2(
520 F2 = (Cl / D2) / (El / E2)
530 PRINT 'THE TOTAL TSS =
540 PRINT "THE ROW RSS = ";Ri
550 PRINT "THE COLUMN CSS = ";Cl
560 PRINT "THE ERROR ESS = ";El
570 PRINT
580 PRINT "THE ROW DEGREES OF FREEDOM =
590 PRINT "THE COLUMN DEGREES OF FREEDOM = ";D2
600 PRINT "THE ERROR DEGREES OF FREEDOM = ";E2
610 PRINT
620 PRINT "THE ROW F-RATIO =
630 PRINT "THE COLUMN F-RATIO = ";F2
700 GOSUB iOOC
800 GOTO 44000
1000 REM
PRINT TO THE PRINTER
1001 IF SLOT = 99 THEN 1205
1002 LOCATE 16,1
1003 COLOR 16,15,0 : PRINT "PRINTING- PLEASE WAIT": COLOR 7,0,0
1005 PAGE = PAGE + 1
1015 SPEED= SPO
1020
1021 LPRINT
1022 LPRINT "STATISTICAL ANALYSIS PACKAGE BUSINESS SOFTWARE, INC."
1025 P$ = "TWO-WAY ANOVA RESULTS"
1026 LPRINT P$; SPC( 74 - LEN (P$));"PAGE "; FNS$ (PAGE)
1030 LPRINT : LPRINT : LPRINT
1032 LPRINT "THE DATA WAS ENTERED.FROM: TI;
1033 IF D$ < > "" THEN LPRINT "FILE--->"D$
1034 IF D$ = " THEN LPRINT "KEYBOARD"
1035
1040 LPRINT "THE TOTAL NUMBER OF ENTRIES: ";Nl
1041 LPRINT : LPRINT : LPRINT
1045 S$ =
1046 FOR 18 = 1 TO 60:S$ = S$ + I*TT: NEXT 18
1047 LPRINT S$
1048 LPRINT "k" SPC( 58);"*"
1049 P$ = * THE TOTAL TSS = IT + STR$ (Ti)
1050 LPRINT P $; SPC( 59 - LEN (P$));"*"
1052 P$ = 1* THE ROW RSS = " + STR$ (Ri)
1053 LPRINT P$; SPC( 59 - LEN (P$)) ; T'*IT
1054 P$ = 1* THE COLUMN CSS = 11 + STR$ (Cl)
1055 LPRINT P$; SPC( 59 - LEN (P$));I*fl
1057 P$ = T* THE ERROR ESS = " + STR$ (El)
1058 LPRINT P$ SPC( 59 - LEN (p$)),II*II
1059 LPRINT T*T; SPC( 58);TT*T'
1060 P$ = 1* THE ROW DEGREES OF FREEDOM = " + STR$ (Dl)
1061 LPRINT P$; SPC( 59 - LEN (P$));IT*II
BASIC
1062 p' = 11* THE COLUMN DEGREES OF FREEDOM = " + STR$ (D2)
1063 LPRINT P $; SPC( 59 - LEN (P$) ) ; "*"
1064 P$ = ?* THE ERROR DEGREES OF FREEFOM " + STR$ (E2)
1065 LPRINT P $; SPC( 59 - LEN ( P $) ) ; "*"
1066 LPRINT "*" ; SPC ( 58);1 1
1067 LPRINT *; SPC( 58);1 1
1068 P$ = "* THE ROW F-RATIO = " ,+ STR$ (Fl)
1069 LPRINT P $; SPC( 59 - LEN (P$));fl*U
1070 P$ = U* THEN COLUMN F-RATIO = " + STR$ (P2)
1071 LPRINT P$; SPC( 59 - LEN (P$));"*"
1074 LPRINT *U; SPC( 58) ;II*I
1075 LPRINT S$
1120 LPRINT : LPRINT : LPRINT : LPRINT
1130 LPRINT "***** END OF OUTPUT
1140 LPRINT CHR$ (12)
1155
1200
1205 LOCATE 16,1 : PRINT SPC(30)
1207 LOCATE 11,1
1210 SPEED= 255
1220 RETURN
44000 PRINT
PRINT
44005 PRINT
PRINT : PRINT : PRINT
44010 PRINT "WOULD YOU LIKE TO MAKE ANOTHER RUN ";: INPUT A$
44020 IF LEFTs ( A $, l ) = "Y" THEN 16
44030 PRINT
PRINT
46000 RUN "STAT"
50000 REM
DATA INPUT FROM FILE TEST
50005 LOCATE 19,1 : GOSUB 63060
50010 A$ = "N":F4 = 0
50015 D$ =
50020 PRINT "IS DATA ENTRY FROM A FILE
50030 INPUT A$
50035 PRINT
50037 IF A$ = "" THEN A$ = "N"
50040 IF LEFT$ (A$,1) = "N" THEN RETURN
50050 IF LEFTs ( A $, 1 ) = "Y" THEN 50100
50060 PRINT CHR$ (7);"ERROR - ENTER A 'Y' OR 'N'"
50070 GOTO 50000
50100 P4 = 1:D$ = "": REM
SET FLAG FOR DATA. INPUT FROM FILE
50105 LOCATE 20,1 : GOSUB 63000
50110 PRINT "ENTER FILENAME.EXT ";: GOSUB 55000
50115 IF D$ = "" THEN RUN "STAT"
50117 ON ERROR GOTO 51000
50200 OPEN "I",#l,D$
50300 INPUT 41 , A9 $, B9 $, A8 $, A7 $, Zi $, Z2 $, Z3 $, Z4 $, Z5$
50310 A = VAL (A9$) :B = VAL ( 39 $) :Al = VAL (P8$) :A2 = VAL (A7$)
50312 REM A=# OF DATA SETS,B=DATA SET TYPE
50315 Ni = A
50320 LOCATE 21,1 : COLOR 0,7,0 : PRINT "FILE: ";D$;" TYPE:
50325 IF B = 1 THEN PRINT "X COUNT:
50330 IF B = 2 THEN CONT "X,Y COUNT:
50340 IF B = 3 THEN PRINT "X,Y,Z COUNT: U;
50345 PRINT A;" ---->"; : COLOR 7,0,0
50350 IF B < > 1 AND B < > 2 AND B < > 3 THEN 51000
50351 REM Z1$=4 ROWS , Z2 $ = # COLS
50353 R = VAL (Z1$) :C = VAL (Z2$)
50355
50357 LOCATE 22,1 : GOSUR 63000 : COLOR 16,15,0 : PRINT "READING IN
DATA SETS": COLOR 7,0,0
50359 J = i:K = 0
50360 FOR I = 1 TO Ni
50390 INPUT i,X$,Y$,Z$:X = VAL ( X $) :Y = VAL ( Y $) : Z = VAL (Z$)
50420 REM PERFORM DATA CALCULATIONS
50430 LOCATE 21,45 : PRINT I;
K=K+1
50431 IF K > C THEN K = 1:J = J + 1
50440 R(J) = R(J) + X:C(K) = C(K) + X
50445 51 = Si + X
50446 S2 = S2 + X * X:S9 = S9 + X
53460
53480 IF K * J = > R * C THEN I = Ni
50500 NEXT I
53505 LOCATE 22,1 : GOSUR 63000
50507 PRINT "ALL DATA POINTS READ IN"
S0510 CLOSE 41
53515 ON ERROR GOTO 60000
53520 RETURN
51000 RESUME 51002 : REM
PRINT ERROR MESSAGE
51002 LOCATE 24,1 : COLOR 16,15,0
51010 PRINT CHR$ (7) ;II***** DATA FILE ERROR *****; : COLOR 7,0,0
51015 POKE 34,0
51050 CLOSE 41
51200 RUN "STAT"
55000 REM
INPUT A LINE
55100 D$ = "":C$ = "":COUNT = 0: LOCATE 20,20 : GOSUB 63000
55200 INPUT D$
55210 RETURN
60000 RESUME 60010
63010 LOCATE 24,1 :COLOR 31,0,0 PRINT CHR $( 7 ) ; :PRINT"***** DATA
ERROR - RETURNING TO MENU *****
60020 COLOR 7,0,0 : RUN "STAT"
61000 RESUME 61010
61010 LOCATE 24,1 : COLOR 31,0,0 : PRINT CHR $( 7 ); : PRINT***** ERROR COULD NOT FIND: PRINTER.DAT *****h;
61020 COLOR 7,0,0 : RUN "STAT"
63000 R99=CSRLIN:C99=POS(0):PRINT SPC(81-C99);:LOCATE R99,C99:RETURN
63060 GOSUB 63000:IF R99=24 THEN RETURN
63070 LOCATE R99+1,1:FOR 199=R99+1 TO 23:PRINT SPACE$(80);:NEXT 199
63080 PRINT SPACE$(79) ; :LOCATE R99,C99:RETURN

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