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Faster uptake and higher photodynamic therapy effect of octagonal
carboxyl phthalocyanine than that of aluminium tetrasulfonated
phthalocyanine
Jin-feng Zhao, Pei-nan Wang and Ji-yao Chen
Introduction
Photosensitizer (PS) of phthalocyanine (Pc) class which has unique properties are widely applicated in
photodynamic therapy (PDT). Especially water-soluble aluminium tetrasulfonated phthalocyanine (AlTSPc),
but its uptake rate is relatively slow. We know that quantum dots (QDs) with carboxylic has good
biocompatibility and has fast uptake character. So in this work, we study whether Octagonal carboxyl
phthalocyanine (Pc-COOH) which is a new synthetic PS has more advantages than AlTSPc or not, such as
faster uptake and higher PDT effect features.
Experiments
2.1 Pc-COOH’s fluorescence and absorption characters
ΦFAl= 0.16
Results
ΦFCOOH= 0.057
3. Uptake
curves and
MTT results
Figure 1. Absorption and fluorescence spectras of the
two samples in aqueous solutions with a concentration of
0.01mg/ml.
Figure 3. Uptake curves in QGY cells
2.2 Fluorescence images
the longer exposure time is
the greater different of cell
viability between the two
samples will be.
Figure 4. Normalized cell viability of Pc-COOH, AlTSPc
and control in QGY cells, and exposure time was 10 min,
30 min and 50 min.
Conclusion
Figure 2. (a) Pc-COOH, (b)AlTSPc, FL images of Pcs in QGY cells
with excition wavelengh of 405 nm. Pcs’s concentration in cells:
0.005mg/ml, drug-culture time: 3 hours, Detection channel: 590nm. Left, FL image; middle, DIC image; right, merged image. Scale
bar is 20 nm.
References
[1] Fischer, A.; Cremer, C.; Stelzer, E. H. K.. Appl. Opt. 1995;
34:1989–2003.
[2] Caroline M Moore*, Doug Pendse and Mark Emberton.
Nature clinical practice urology, Rev. 2009; l 6:18-30.
1.
2.
Uptake rate of Pc-COOH is faster than
AlTSPc’s (fluorescence relatively large
difference between the two Pcs at 3 h).
Phototoxic effect of AlTSPc is lower
than Pc-COOH’s for every different
exposure time, in particular, for 50 min
exposure time.

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