A Molecular Look at Environmental Interactions Saturday March 26th 12:30-‐3:30 Kindschi Hall of Science Room 1110 qRT PCR Results Gene Expression Microarray Results RNA h p://salmonellatyphi.org/ h ps:/h p://www.5prime.com/select-country.aspx /sophs-human-body.wikispaces.com/Circulatorty+System The First Annual Cell and Molecular Biology Research Symposium Diagnostics of Typhoid Fever, a bacteremia prevalent in underdeveloped countries, lack reliability. RNA was analyzed via Gene Expression Microarray and qRT– PCR from different infectious stages We found 179 and 175 differentially-expressed genes between acute/convalescent and acute/recovery Genes of the immune system showed expression reflected the host’s response to the infectious stages Sarah Borwn The Cell and Molecular Biology department is holding the First Annual CMB Senior Research Symposium. Come hear talks from the gradua>ng seniors on the research they’ve done during their >me at Grand Valley. • ? 43/89+ • • • 0/8+ Register for the event here: w w w . g v s u . e d u / c m b / fi r s t -‐ a n n u a l -‐ c e l l -‐ molecular-‐biology-‐91.htm Regional variances in Tachycineta bicolor that are positive for malarial infection. Gel electrophoresis results reveal samples positive for malaria in blood. Negative samples are possibly still positive, but malaria is in different stage of development. Future experiments involve liver testing to find presence of malaria. Britney Bunker Jennifer Grousd Refreshments and lunch will be provided! Brooke Prieskorn Effective population size (Ne) can mimic genetic drift of a population by using microsatellite data Extended Bayesian Skyline Plot (EBSP) determines historical Ne values and previous size changes Virginia big-eared bats are not affected by White-nose Syndrome and may have increased in size A constant population size cannot be rejected, however a slight increase in Ne occurs APW(ArtificialPondWater)Gravitropism Ricerootsobservedunderblueatalightintensityof2.4µEover21hours. BarreG Kyle Lightsource Ub Ub E2 Cul3 RBX Dark Red E3 Ubiquitin ligase LRB associates with scaffold protein Cul3 in red light but not in the dark LRB was successfully purified in-vivo from Arabidopsis thaliana tissue in varying light conditions Preliminary evidence suggests that the association between LRB and Cul3 decreases in far-red light Activation and association of LRB complex members may be dependent on light conditions Carly Wiersma GravityVector 0minutes 300minutes 600minutes 900minutes 1260minutes 5%OptiprepGravitropism Ricerootsobservedunderblueatalightintensityof2.4µEover21hours. Lightsource GravityVector 0minutes 300minutes 600minutes 900minutes 1260minutes • Thestarch-statolithmodelistheacceptedmodelforgravitropism,butweproposea gravitationalpressuremodel • RicerootsshowanegativephototropismandpositivegravitropisminArtificalPondWater (APW). • Ricerootsshowonlyanegativephototropismin10%Optiprep. • Changingthedensityofthemediumaffectsthegravitropicresponseofthericeroots.
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